Mutagenetic and electron microscopy analysis of actin filament severing by Cordon-Bleu, a WH2 domain protein.
Gif-sur-Yvette, France. In Cytoskeleton (hoboken), 2014
To understand which primary sequence elements determine the filament severing activity of the WH2 repeats, here we combine a mutagenetic/domain swapping approach of the minimal fully active Cobl-KAB construct, which comprises the lysine rich region K preceding the two first WH2 domains A and B. The mutated Cobl constructs display variable loss of the original filament nucleating activities of native Cobl-KAB, without any strict correlation with a loss in actin binding, which emphasizes the functional importance of the electrostatic environment of WH2 domains.
p21(Waf1/Cip1) deficiency stimulates centriole overduplication.
Pittsburgh, United States. In Cell Cycle, 2006
Using a novel marker for maternal centrioles, Cep170, we show here that knock-down of p21 protein expression in murine myeloblasts can stimulate excessive centriole numbers in the presence of only one mature centriole.
[Molecular and biophysical aspects of potassium channels].
Ōsaka, Japan. In Nihon Rinsho, 1996
Several types of inwardly rectifying K+ channels, containing two putative transmembrane domains in each subunit, have recently been cloned and can be classified into four groups: 1) IRK family; classical inward rectifying, 2) GIRK family; G-protein-activated, 3) KATP family; ATP-inhibited, and 4) KAB family; ATP-dependent.