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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Aug 2016.

ISA1 Isa1p

ISA1, Isa1p, ISCA1, AtISA1
ISCA1 is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions (Cozar-Castellano et al., 2004 [PubMed 15262227]).[supplied by OMIM, Mar 2008] (from NCBI)
Top mentioned proteins: HAD, Isa, CAN, ACID, DSS1
Papers on ISA1
Amylopectin biosynthetic enzymes from developing rice seed form enzymatically active protein complexes.
Fujita et al., Akita, Japan. In J Exp Bot, Aug 2015
SSIIa, SSIIIa, SSIVb, BEI, BEIIb, and PUL co-eluted at mass sizes >700kDa, and SSI, SSIIa, BEIIb, ISA1, PUL, and Pho1 co-eluted at 200-400kDa.
TILLING mutants of durum wheat result in a high amylose phenotype and provide information on alternative splicing mechanisms.
Lafiandra et al., Viterbo, Italy. In Plant Sci, Apr 2015
The complete silencing of these genes had a number of pleiotropic effects on starch synthesis: it affected the transcriptional activity of SBEIIb, ISA1 (starch debranching enzyme) and all of the genes encoding starch synthases (SSI, SSIIa, SSIII and GBSSI).
ISCA2 mutation causes infantile neurodegenerative mitochondrial disorder.
Kaya et al., Riyadh, Saudi Arabia. In J Med Genet, Mar 2015
RESULTS: We identified a homoallelic missense founder mutation in ISCA2 leading to mitochondrial depletion and reduced complex I activity as well as decreased ISCA2, ISCA1 and IBA57 expression in fibroblasts.
Molecular characterization of an isoamylase 1-type starch debranching enzyme (DBEI) in grain amaranth (Amaranthus cruentus L.).
Nemoto et al., Nagano, Japan. In Mol Biol Rep, 2014
This amaranth DBE appears to be an ISA1-type DBE (DBEI), based on its substrate specificity and the sequence similarity between the 2,391-bp cDNA clone and ISA1 s from potato and Arabidopsis.
Identification of genomic regions and the isoamylase gene for reduced grain chalkiness in rice.
Yu et al., Wuhan, China. In Plos One, 2014
Residing on the qPGC8-2 region, the isoamylase gene (ISA1) was preferentially expressed in the endosperm and revealed some nucleotide polymorphisms between two varieties, Nipponbare and ZS97.
Formation of [4Fe-4S] clusters in the mitochondrial iron-sulfur cluster assembly machinery.
Banci et al., Napoli, Italy. In J Am Chem Soc, 2014
The generation of [4Fe-4S] clusters in mitochondria critically depends, in both yeast and human cells, on two A-type ISC proteins (in mammals named ISCA1 and ISCA2), which perform a nonredundant functional role forming in vivo a heterocomplex.
Crystal structure of the Chlamydomonas starch debranching enzyme isoamylase ISA1 reveals insights into the mechanism of branch trimming and complex assembly.
Palcic et al., Villeneuve-d'Ascq, France. In J Biol Chem, 2014
The starch debranching enzymes isoamylase 1 and 2 (ISA1 and ISA2) are known to exist in a large complex and are involved in the biosynthesis and crystallization of starch.
[2Fe-2S] cluster transfer in iron-sulfur protein biogenesis.
Winkelmann et al., Sesto Fiorentino, Italy. In Proc Natl Acad Sci U S A, 2014
Here we show that the structural plasticity of the dimeric state of the [2Fe-2S] bound form of human GRX5 (holo hGRX5) is the crucial factor that allows an efficient cluster transfer to the partner proteins human ISCA1 and ISCA2 by a specific protein-protein recognition mechanism.
Use of advanced recombinant lines to study the impact and potential of mutations affecting starch synthesis in barley.
Smith et al., Norwich, United Kingdom. In J Cereal Sci, 2014
The lys5f (ADPglucose transporter), wax (granule-bound starch synthase), isa1 (debranching enzyme isoamylase 1) and sex6 (starch synthase IIa) mutations were introgressed into NFC Tipple to give mutant and wild-type BC2F4 families with different genomic contributions of the donor parent.
FLOURY ENDOSPERM6 encodes a CBM48 domain-containing protein involved in compound granule formation and starch synthesis in rice endosperm.
Wan et al., Nanjing, China. In Plant J, 2014
Furthermore, FLO6 can interact with isoamylase1 (ISA1) both in vitro and in vivo, whereas ISA1 does not bind to starch directly.
Malfunctioning of the iron-sulfur cluster assembly machinery in Saccharomyces cerevisiae produces oxidative stress via an iron-dependent mechanism, causing dysfunction in respiratory complexes.
Campos-García et al., Nicolás Romero, Mexico. In Plos One, 2013
Raman spectroscopy analysis and immunoblotting indicated that in mitochondria from SSQ1 and ISA1 mutants, the content of [Fe-S] centers was decreased, as was formation of Rieske protein-dependent supercomplex III2IV2, but this was not observed in the iron-deficient ATX1 and MRS4 mutants.
Replacement of the endogenous starch debranching enzymes ISA1 and ISA2 of Arabidopsis with the rice orthologs reveals a degree of functional conservation during starch synthesis.
Zeeman et al., Zürich, Switzerland. In Plos One, 2013
Here, we tested whether the specificities of the debranching enzymes (ISA1 and ISA2) are major determinants of species-dependent differences in amylopectin structure by replacing the dicotyledonous Arabidopsis isoamylases (AtISA1 and AtISA2) with the monocotyledonous rice (Oryza sativa) isoforms.
Distinct functional properties of isoamylase-type starch debranching enzymes in monocot and dicot leaves.
Wattebled et al., Villeneuve-d'Ascq, France. In Plant Physiol, 2013
Isoamylase-type starch debranching enzymes (ISA) play important roles in starch biosynthesis in chloroplast-containing organisms, as shown by the strict conservation of both catalytically active ISA1 and the noncatalytic homolog ISA2.
The heteromultimeric debranching enzyme involved in starch synthesis in Arabidopsis requires both isoamylase1 and isoamylase2 subunits for complex stability and activity.
Zeeman et al., Zürich, Switzerland. In Plos One, 2012
Mutants of several species lacking the ISA1-subclass of isoamylase are impaired in amylopectin synthesis.
The human mitochondrial ISCA1, ISCA2, and IBA57 proteins are required for [4Fe-4S] protein maturation.
Lill et al., Marburg an der Lahn, Germany. In Mol Biol Cell, 2012
ISCA1, ISCA2, and IBA57 were depleted by RNA interference. Depleted cells contained massively swollen and enlarged mitochondria that were virtually devoid of cristae membranes, demonstrating the importance of these proteins for mitochondrial biogenesis.
Specialized function of yeast Isa1 and Isa2 proteins in the maturation of mitochondrial [4Fe-4S] proteins.
Lill et al., Marburg an der Lahn, Germany. In J Biol Chem, 2012
define Isa1, Isa2, and Iba57 as a specialized, late-acting ISC assembly subsystem that is specifically dedicated to the maturation of mitochondrial [4Fe-4S] proteins.
Iron-binding activity of human iron-sulfur cluster assembly protein hIscA1.
Ding et al., Wenzhou, China. In Biochem J, 2010
Iron-binding activity of human iron-sulfur cluster assembly protein hIscA1
Monothiol glutaredoxin Grx5 interacts with Fe-S scaffold proteins Isa1 and Isa2 and supports Fe-S assembly and DNA integrity in mitochondria of fission yeast.
Roe et al., Seoul, South Korea. In Biochem Biophys Res Commun, 2010
These results indicate that Grx5 plays a central role in Fe-S assembly process through interaction with A-type Fe-S scaffold proteins Isa1 and Isa2.
Human ISCA1 interacts with IOP1/NARFL and functions in both cytosolic and mitochondrial iron-sulfur protein biogenesis.
Lee et al., Philadelphia, United States. In J Biol Chem, 2010
human IscA1 plays an important role in both mitochondrial and cytosolic iron-sulfur cluster biogenesis, and a notable component of the latter is the interaction between IscA1 and IOP1.
Discovery of genes essential for heme biosynthesis through large-scale gene expression analysis.
Mootha et al., Boston, United States. In Cell Metab, 2009
Required for functional heme biosynthesis in erythroid cells.
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