gopubmed logo
find other proteinsAll proteins
GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Aug 2016.

DNA fragmentation factor, 45kDa, alpha polypeptide

ICAD, DFF45, inhibitor of CAD, DFFA
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: PrP, caspase-3, POLYMERASE, CAN, Caldesmon
Papers using ICAD antibodies
JNK suppresses apoptosis via phosphorylation of the proapoptotic Bcl-2 family protein BAD
Ellis L M et al., In British Journal of Cancer, 2003
... (C-17) antibody (Santa Cruz Biotechnology), monoclonal mouse anti-vinculin (Sigma, St Louis, MO, USA), polyclonal rabbit anti-DFF45 (BD Biosciences, San Diego, CA, USA), ...
Resistance to Granzyme B-mediated Cytochrome c Release in Bak-deficient Cells
Rabinowich Hannah et al., In The Journal of Experimental Medicine, 1997
... Cbz-Val-Ala-Asp-fluoromethyl ketone (Z-VAD-FMK) were from Enzyme System; rabbit anti-DNA fragmentation factor (DFF)45/inhibitor of caspase-activate DNase (ICAD) Ab was from ProSci.
Papers on ICAD
ERβ up-regulation was involved in silibinin-induced growth inhibition of human breast cancer MCF-7 cells.
Ikejima et al., Shenyang, China. In Arch Biochem Biophys, Feb 2016
Apoptotic cascades, including the sequential activation of caspase-9 and -6, and finally the cleavage of caspase substrates, PARP and ICAD, caused by treatment with silibinin, were all repressed by PHTPP pre-treatment but exacerbated by DPN.
Heart Failure With Preserved Ejection Fraction Induces Beiging in Adipose Tissue.
Sam et al., Boston, United States. In Circ Heart Fail, Jan 2016
In HFpEF, despite a larger brown AT mass (5.96 versus 4.50 mg/g), brown AT showed reduced activity with decreased uncoupling protein 1 (ucp-1), cell death-inducing DFFA-like effector a (cidea), and epithelial V-like antigen (eva) expression and decreased expression of lipolytic enzymes (hormone-sensitive lipase, lipoprotein lipase, and fatty acid binding protein 4) versus sham.
High potential of adhesion to biotic and abiotic surfaces by opportunistic Staphylococcus aureus strains isolated from orthodontic appliances.
Mastouri et al., Monastir, Tunisia. In Microb Pathog, Dec 2015
In addition, the presence of icaA and icaD genes was achieved by polymerase chain reaction (PCR).
The role of peptidylarginine deiminase 4 in ovarian cancer cell tumorigenesis and invasion.
Chang et al., Jinan, China. In Tumour Biol, Dec 2015
PCR arrays of A2780 cells treated with PADI4-siRNA revealed the up-regulated expression of six genes, including cell death-inducing DFFA-like effector a (CIDEA) and tumor necrosis factor receptor superfamily member 9 (TNFRSF9), and the down-regulation of seven genes, including integrin beta 3 (ITGB3) and BCL2-antagonist/killer 1 (BAK1).
Structural insight into CIDE domains: the Janus face of CIDEs.
Park, Kyŏngsan, South Korea. In Apoptosis, Feb 2015
Cell-death inducing DFF45-like effect domain (CIDE domain) is a protein interaction module that was initially found in DNA fragmentation factor (DFF) proteins DFF40 and DFF45.
Polysaccharide intercellular adhesin in biofilm: structural and regulatory aspects.
Montanaro et al., Bologna, Italy. In Front Cell Infect Microbiol, 2014
The product of icaA is an N-acetylglucosaminyltransferase that synthetizes PIA oligomers from UDP-N-acetylglucosamine. The product of icaD gives optimal efficiency to IcaA.
Antibiotic Susceptibility of Biofilm Cells and Molecular Characterisation of Staphylococcus hominis Isolates from Blood.
Garza-González et al., Monterrey, Mexico. In Plos One, 2014
OBJECTIVES: We aimed to characterise the staphylococcal cassette chromosome mec (SCCmec) type, genetic relatedness, biofilm formation and composition, icaADBC genes detection, icaD expression, and antibiotic susceptibility of planktonic and biofilm cells of Staphylococcus hominis isolates from blood.
[Research advances on CIDEC in insulin resistance].
Xiao et al., Xi'an, China. In Zhongguo Dang Dai Er Ke Za Zhi, 2013
Recent studies have indicated that cell death-inducing DFF45-like effector C (CIDEC) is responsible for the development of insulin resistance.
Lipid droplet metabolism.
Kraemer et al., Stanford, United States. In Curr Opin Clin Nutr Metab Care, 2013
Cell death-inducing DFF45-like effector proteins are not only involved in lipid droplet expansion, but also in the cellular response to stress and lipid secretion.
DFF45 expression in human endometrium is associated with menstrual cycle phases and decreases after menopause.
Grabowska et al., Kraków, Poland. In Gynecol Obstet Invest, 2011
The DFF45 level in human endometrium corresponds to the respective phase of the menstrual cycle and decreases significantly after menopause.
[Novel mechanism for hepatic lipid accumulation: a physiological role for hepatic PPARγ-fsp27 signal].
Matsusue, Fukuoka, Japan. In Yakugaku Zasshi, 2011
Fsp27 and cell death-inducing DFF45-like effector (CIDE) C, the human homologue of fsp27, belong to the CIDE family.
Histone H1 subtype preferences of DFF40 and possible nuclear localization of DFF40/45 in normal and trichostatin A-treated NB4 leukemic cells.
Sourlingas et al., Ayía Paraskeví, Greece. In Apoptosis, 2010
The heterodimer, DFF40-DFF45, is localized to the chromatin fraction under apoptotic as well as non-apoptotic conditions.
MiR-145, a new regulator of the DNA fragmentation factor-45 (DFF45)-mediated apoptotic network.
Chen et al., Shanghai, China. In Mol Cancer, 2009
study reveals a previously unrecognized function of miR-145 in DFF45 processing, which may underlie crucial aspects of cancer biology
DFF45 expression in ovarian endometriomas.
Basta et al., Kraków, Poland. In Eur J Obstet Gynecol Reprod Biol, 2009
A decreased level of DFF45 observed in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression.
1-Methyl-4-phenyl-pyridinium ion-induced oxidative stress, c-Jun phosphorylation and DNA fragmentation factor-45 cleavage in SK-N-SH cells are averted by selegiline.
Ebadi et al., Thailand. In Neurochem Int, 2008
MPP(+) did not change the total levels of c-Jun but enhanced phosphorylation of c-Jun at Ser73 and cleavage of DNA fragmentation factor 45, which were diminished by selegiline.
Cidea-deficient mice have lean phenotype and are resistant to obesity.
Li et al., Singapore, Singapore. In Nat Genet, 2003
We show here that Cidea, a protein of unknown function sharing sequence similarity with the N-terminal region of DNA fragmentation factors Dffb and Dffa, is expressed at high levels in BAT.
Endonuclease G is an apoptotic DNase when released from mitochondria.
Wang et al., Dallas, United States. In Nature, 2001
One such nuclease, DNA fragmentation factor (DFF, a caspase-activated deoxyribonuclease (CAD) and its inhibitor (ICAD)), is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 (refs 2,3,4).
DFF45/ICAD can be directly processed by granzyme B during the induction of apoptosis.
Ley et al., Saint Louis, United States. In Immunity, 2000
In this report, we demonstrate that GzmB can initiate apoptosis in the absence of caspase-3 activity by directly cleaving DFF45/ICAD to liberate activated DFF40/CAD.
Solution structure of the CIDE-N domain of CIDE-B and a model for CIDE-N/CIDE-N interactions in the DNA fragmentation pathway of apoptosis.
Wagner et al., Cambridge, United States. In Cell, 2000
Apoptotic DNA fragmentation and chromatin condensation are mediated by the caspase-activated DFF40/ CAD nuclease, which is chaperoned and inhibited by DFF45/ICAD.
Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis.
Nagata et al., Suita, Japan. In Nature, 1998
In the accompanying Article, we have identified and molecularly cloned a caspase-activated deoxyribonuclease (CAD) and its inhibitor (ICAD).
share on facebooktweetadd +1mail to friends