Reconstitution of mitotic chromatids with a minimum set of purified factors.
Wako, Japan. In Nat Cell Biol, Aug 2015
We find that octameric nucleosomes containing the embryonic variant H2A.X-F are highly susceptible to FACT and function as the most productive substrate for subsequent actions of topo II and condensin I. Cdk1 phosphorylation of condensin I is the sole mitosis-specific modification required for chromatid reconstitution.
Chromatin Dynamics in Vivo: A Game of Musical Chairs.
Bethesda, United States. In Genes (basel), Dec 2014
In this review, we will focus on the localization of the major histone variants H3.3, CENP-A, H2A.Z, and macroH2A, as well as how these variants have evolved, their structural differences, and their functional significance in vivo.
The Structural Determinants behind the Epigenetic Role of Histone Variants.
Victoria, Canada. In Genes (basel), Dec 2014
A picture starts to emerge in which histone H2A variants, in addition to their individual specific contributions to the nucleosome structure and dynamics, globally impair the accessibility of linker histones to defined chromatin locations and may have important consequences for determining different states of chromatin metabolism.
RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling.
More papers using
Amsterdam, Netherlands. In Cell, 2012
Ubiquitin-dependent signaling during the DNA damage response (DDR) to double-strand breaks is initiated by two E3 ligases, RNF8 and RNF168, targeting histone H2A and H2AX. Study shows that ubiquitin chains per se are insufficient for signaling, but RNF168 target ubiquitination is required for DDR damage.
Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster.
In PLoS ONE, 2005
... Cruz Biotechnology), Ki67 (Lab Vision, Fremont, CA, USA); mouse monoclonal antibodies for phosphor-H2AX, Ser 139, (γH2AX) (BioLegend, San Diego, CA, USA), ... Histochemical localization of alkaline phosphatase activity in decalcified bone and cartilage
In Scientific Reports, 2001
... Primary antibodies CD34, CD44, CD105, CD29, SCA-1, CD45, osteocalcin, 53BP1, γ-H2AX, were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA) ...