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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 31 Oct 2014.

H2A histone family, member X

H2A, H2AX
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: Histone, CAN, p53, Atm, V1a
Papers using H2A antibodies
Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by silica nanomaterials in human neuronal cell line
Supplier
Celotti Lucia et al., In Analytical and Bioanalytical Chemistry, 2009
... Anti-γ-H2AX mouse monoclonal antibody was purchased from Upstate, Millipore (Billerica, MA, USA) and from Molecular Probes (Alexa Fluor, Life Technologies, Carlsbad, CA, USA) ...
Prediction and functional analysis of native disorder in proteins from the three kingdoms of life.
Supplier
Lustig Arthur J., In PLoS ONE, 2009
... Human H2A peptides 1–100 (ab27894) and 100-end (ab15660) were from Abcam.
Parameters of oligonucleotide-mediated gene modification in mouse ES cells
Supplier
Kmiec Eric B et al., In Molecular therapy. Nucleic acids, 2009
... antibodies were added at a dilution of 1:100 in 10% milk and kept at 4 °C overnight (γH2AX: Cell Signaling, Boston, MA; PCNA: Santa ...
Aggresome-forming TTRAP mediates pro-apoptotic properties of Parkinson's disease-associated DJ-1 missense mutations
Supplier
Li R et al., In Oncogene, 2008
... (Danvers, MA, USA); phospho-JNK1/2/3(sc-6254), MYC(sc-764) and γ-H2AX(sc-101696) from Santa Cruz Biotechnology, Inc ...
Noxa mediates bortezomib induced apoptosis in both sensitive and intrinsically resistant mantle cell lymphoma cells and this effect is independent of constitutive activity of the AKT and NF-kappaB pathways.
Supplier
Hofmann Andreas, In PLoS ONE, 2007
... out using the following antibodies: FLAG antibody (Sigma), NOXA antibody (EMD), ATF3 (Santa Cruz), histone H2A (Abcam).
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Papers on H2A
Inhibition of EGFR or IGF-1R signaling enhances radiation response in head and neck cancer models but concurrent inhibition has no added benefit.
New
Skinner et al., Houston, United States. In Cancer Med, 30 Nov 2014
Formation of nuclear γ-H2AX and 53BP1 foci was monitored by immunofluorescence.
γ-H2AX foci as in vivo effect biomarker in children emphasize the importance to minimize x-ray doses in paediatric CT imaging.
New
Thierens et al., Gent, Belgium. In Eur Radiol, 30 Nov 2014
DNA damage was estimated by scoring γ-H2AX foci in peripheral blood T lymphocytes.
Crystal structure of the PRC1 ubiquitylation module bound to the nucleosome.
New
Impact
Tan et al., United States. In Nature, 29 Nov 2014
This group includes the Polycomb repressive complex 1 (PRC1), which ubiquitylates nucleosomal histone H2A Lys 119 using its E3 ubiquitin ligase subunits, Ring1B and Bmi1, together with an E2 ubiquitin-conjugating enzyme, UbcH5c.
A Phase I study of veliparib (ABT-888) in combination with low-dose fractionated whole abdominal radiation therapy in patients with advanced solid malignancies and peritoneal carcinomatosis.
New
Azad et al., Hopkins, United States. In Clin Cancer Res, 29 Nov 2014
Circulating tumor cells (CTCs) were collected and evaluated for ɣ-H2AX.
Synchrotron-based Imaging of Chromium and γ-H2AX Immunostaining in the Duodenum Following Repeated Exposure to Cr(VI) in Drinking Water.
New
Harris et al., Mission Viejo, United States. In Toxicol Sci, 28 Nov 2014
DNA damage was also assessed by γ-H2AX immunostaining.
Tyrosine phosphorylation of histone H2A by CK2 regulates transcriptional elongation.
New
Impact
Rosenfeld et al., San Diego, United States. In Nature, 24 Oct 2014
This regulation is based on the phosphorylation of a highly conserved tyrosine residue, Tyr 57, in histone H2A and is mediated by the unsuspected tyrosine kinase activity of casein kinase 2 (CK2).
Histone H2A.Z subunit exchange controls consolidation of recent and remote memory.
New
Impact
Sweatt et al., Birmingham, United States. In Nature, 14 Oct 2014
Here we show that histone H2A.Z, a variant of histone H2A, is actively exchanged in response to fear conditioning in the hippocampus and the cortex, where it mediates gene expression and restrains the formation of recent and remote memory.
Histone variant H2A.X deposition pattern serves as a functional epigenetic mark for distinguishing the developmental potentials of iPSCs.
New
Impact
Xiao et al., New Haven, United States. In Cell Stem Cell, 04 Oct 2014
Here we show that the histone variant H2A.X is a functional marker that can distinguish the developmental potentials of mouse iPSC lines.
The developmental potential of iPSCs is greatly influenced by reprogramming factor selection.
New
Impact
Jaenisch et al., Jerusalem, Israel. In Cell Stem Cell, 04 Oct 2014
Although differentially methylated regions, transcript number of master regulators, establishment of specific superenhancers, and global aneuploidy were comparable between high- and low-quality lines, aberrant gene expression, trisomy of chromosome 8, and abnormal H2A.X deposition were distinguishing features that could potentially also be applicable to human.
Environmental responses mediated by histone variants.
Review
New
Henikoff et al., Seattle, United States. In Trends Cell Biol, 20 Sep 2014
Phosphorylation of histone H2A.X rapidly marks double-strand DNA breaks for chromatin repair, which is mediated by both H2A and H3 histone variants.
γ-H2AX as a biomarker for DNA double-strand breaks in ecotoxicology.
Review
New
Garaj-Vrhovac et al., Zagreb, Croatia. In Ecotoxicol Environ Saf, Jul 2014
Soon after the occurrence of a DNA double-strand break (DSB), the formation of γ-H2AX histone variants is to be expected.
H2A-DUBbing the mammalian epigenome: expanding frontiers for histone H2A deubiquitinating enzymes in cell biology and physiology.
Review
New
Nijnik et al., Canada. In Int J Biochem Cell Biol, May 2014
Posttranslational modifications of histone H2A through the attachment of ubiquitin or poly-ubiquitin conjugates are common in mammalian genomes and play an important role in the regulation of chromatin structure, gene expression, and DNA repair.
MiR-101 Induces Senescence and Prevents Apoptosis in the Background of DNA Damage in MCF7 Cells.
New
Bamezai et al., Jammu, India. In Plos One, Dec 2013
UNLABELLED: Moderately increased DNA damage due to the exogenous miR-101 (4 fold) over-expression in MCF7 cells was substantiated by an increase in the number of γ-H2AX foci, correlating with a simple-to-do Halo-assay.
Dynamic regulation and function of histone monoubiquitination in plants.
Review
New
Shen et al., Strasbourg, France. In Front Plant Sci, Dec 2013
While H2A monoubiquitination (H2Aub1) is generally associated with transcription repression mediated by the Polycomb pathway, H2Bub1 is involved in transcription activation.
Histone exchange and histone modifications during transcription and aging.
Review
New
Tyler et al., Houston, United States. In Biochim Biophys Acta, Mar 2013
The basic repeating unit of chromatin is the nucleosome, which includes 147 bp of DNA wrapped 1.65 times around an octamer of core histone proteins comprising two molecules each of H2A, H2B, H3 and H4.
RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling.
Impact
GeneRIF
Sixma et al., Amsterdam, Netherlands. In Cell, 2012
Ubiquitin-dependent signaling during the DNA damage response (DDR) to double-strand breaks is initiated by two E3 ligases, RNF8 and RNF168, targeting histone H2A and H2AX. Study shows that ubiquitin chains per se are insufficient for signaling, but RNF168 target ubiquitination is required for DDR damage.
Redundant and nonredundant functions of ATM and H2AX in αβ T-lineage lymphocytes.
GeneRIF
Bassing et al., Philadelphia, United States. In J Immunol, 2012
Both redundant and nonredundant functions of H2AX and ATM transgenes are required for normal recombination of T cell receptor (TCR) loci, proliferative expansion of developing thymocytes, and maintenance of genomic stability in cycling alphabeta T-lineage cells.
High basal γH2AX levels sustain self-renewal of mouse embryonic and induced pluripotent stem cells.
GeneRIF
Giachino et al., Torino, Italy. In Stem Cells, 2012
Findings suggest a novel function of H2AX that expands the knowledge of this histone variant beyond its role in DNA damage and into a new specialized biological function in mouse pluripotent stem cells.
Deregulation of BRCA1 leads to impaired spatiotemporal dynamics of γ-H2AX and DNA damage responses in Huntington's disease.
GeneRIF
Ryu et al., Seoul, South Korea. In Mol Neurobiol, 2012
BRCA1 directly modulates the spatiotemporal dynamics of gamma-H2AX upon genotoxic stress and serves as a molecular maker for neuronal DNA damage response in Huntington's disease.
Loss of CCDC6, the first identified RET partner gene, affects pH2AX S139 levels and accelerates mitotic entry upon DNA damage.
GeneRIF
Celetti et al., Napoli, Italy. In Plos One, 2011
Following genotoxic stress, loss or inactivation of CCDC6 in cancers that carry the CCDC6 fusion, accelerates the dephosphorylation of pH2AX S139, resulting in defective G2 arrest and premature mitotic entry.
More papers using H2A antibodies
Complementary functions of ATM and H2AX in development and suppression of genomic instability
Supplier
Malle Ernst et al., In DNA Repair, 2007
... , termed γ-H2AX, Cell Signaling Technology, Beverly, MA, USA); ...
Enhanced inhibition of adipogenesis and induction of apoptosis in 3T3-L1 adipocytes with combinations of resveratrol and quercetin
Supplier
Cook John A. et al., In Frontiers in Oncology, 2007
... CA, USA); mouse monoclonal anti-phospho histone H2AX (Ser139), clone JBW301, and rabbit antiserum to histone H2A (acidic patch) were from Upstate Cell Signaling Solutions (Temecula, CA, USA) ...
Integrin-mediated transforming growth factor-beta activation regulates homeostasis of the pulmonary epithelial-mesenchymal trophic unit
Supplier
Kuwano Kazuyoshi et al., In Oncoimmunology, 2005
... used were rabbit anti-LC3 (Novus), rabbit anti-ATG5 (Novus), mouse anti-p21 (Cell signaling Technology), rabbit anti-phospho-histone H2A.X (Ser139) (Cell signaling Technology), mouse anti-poly and ...
Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster.
Supplier
Oshima Robert, In PLoS ONE, 2005
... Cruz Biotechnology), Ki67 (Lab Vision, Fremont, CA, USA); mouse monoclonal antibodies for phosphor-H2AX, Ser 139, (γH2AX) (BioLegend, San Diego, CA, USA), ...
The comet assay: a method to measure DNA damage in individual cells
Supplier
Fritz G et al., In Cell Death & Disease, 2005
... H2AX anti-rabbit antibody (pS139) (Epitomics, Burlingame, CA, USA), ERK2 ...
Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair.
Supplier
Kirkpatrick David T., In PLoS ONE, 2004
... anti-CHK2 (Santa Cruz), anti-phospho-CHK1 (Ser 345) (Cell signaling), anti-phospho-CHK2 (Thr 68) (Cell signaling), anti-γH2AX (Upstate), anti-H2AX (Abcam), anti-HA (HA-7, Sigma) ...
Histochemical localization of alkaline phosphatase activity in decalcified bone and cartilage
Supplier
Tang Liping et al., In Scientific Reports, 2001
... Primary antibodies CD34, CD44, CD105, CD29, SCA-1, CD45, osteocalcin, 53BP1, γ-H2AX, were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA) ...
Functional interaction between BLM helicase and 53BP1 in a Chk1-mediated pathway during S-phase arrest
Supplier
Harris Curtis C. et al., In The Journal of Cell Biology, 2001
... Anti-γ-H2AX: rabbit polyclonal PC-100 (Trevigen).
Crystal structure of a nucleosome core particle containing the variant histone H2A.Z
Supplier
Chrivia John C. et al., In International Journal of Cell Biology, 1999
... Other commercial antibodies were histone H2A.Z polyclonal (Abcam, ab4174), histone H3 polyclonal ...
A peptidomimetic inhibitor of farnesyl:protein transferase blocks the anchorage-dependent and -independent growth of human tumor cell lines
Supplier
Nie Daotai et al., In Cancer Cell International, 1994
... from Clontech; Lamin A/C (sc-20681) and emerin antibodies were purchased from Santa Cruz biotechnology; anti-phosphorylated histone-H2AX polyclonal antibody was from Trevigen.
Methods for clonal growth and serial cultivation of normal human epidermal keratinocytes and mesothelial cells.
Supplier
Karl Mike O., In PLoS ONE, 1988
... (Abcam), anti Keratin 1,10,11 (Ks 8.60; Abcam), anti Keratin 10 (RKSE60; Abcam), anti-keratin 1 (AF87; Covance), anti-γH2AX (Ser139; Millipore), anti-BrdU (B44; BD Biosciences), anti-phospho-histone H3 (P-H3; Upstate ...
Changes in the cell kinetics of pig epidermis after single doses of X rays.
Supplier
Borgmann Kerstin, In PLoS ONE, 1987
... Mouse anti-γ-H2AX, JBW3011/500Milipore ...
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