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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 29 Jul 2015.

H2A histone family, member X

H2A, H2AX
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: Histone, CAN, Atm, p53, V1a
Papers using H2A antibodies
Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by silica nanomaterials in human neuronal cell line
Supplier
Celotti Lucia et al., In Analytical and Bioanalytical Chemistry, 2009
... Anti-γ-H2AX mouse monoclonal antibody was purchased from Upstate, Millipore (Billerica, MA, USA) and from Molecular Probes (Alexa Fluor, Life Technologies, Carlsbad, CA, USA) ...
Prediction and functional analysis of native disorder in proteins from the three kingdoms of life.
Supplier
Lustig Arthur J., In PLoS ONE, 2009
... Human H2A peptides 1–100 (ab27894) and 100-end (ab15660) were from Abcam.
Parameters of oligonucleotide-mediated gene modification in mouse ES cells
Supplier
Kmiec Eric B et al., In Molecular therapy. Nucleic acids, 2009
... antibodies were added at a dilution of 1:100 in 10% milk and kept at 4 °C overnight (γH2AX: Cell Signaling, Boston, MA; PCNA: Santa ...
Aggresome-forming TTRAP mediates pro-apoptotic properties of Parkinson's disease-associated DJ-1 missense mutations
Supplier
Li R et al., In Oncogene, 2008
... (Danvers, MA, USA); phospho-JNK1/2/3(sc-6254), MYC(sc-764) and γ-H2AX(sc-101696) from Santa Cruz Biotechnology, Inc ...
Noxa mediates bortezomib induced apoptosis in both sensitive and intrinsically resistant mantle cell lymphoma cells and this effect is independent of constitutive activity of the AKT and NF-kappaB pathways.
Supplier
Hofmann Andreas, In PLoS ONE, 2007
... out using the following antibodies: FLAG antibody (Sigma), NOXA antibody (EMD), ATF3 (Santa Cruz), histone H2A (Abcam).
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Papers on H2A
Salinomycin radiosensitizes human nasopharyngeal carcinoma cell line CNE-2 to radiation.
New
Chen et al., Zhangjiagang, China. In Tumour Biol, 25 Aug 2015
DNA damage was detected via γ-H2AX foci counting.
A Mouse Ear Model for Bystander Studies Induced by Microbeam Irradiation.
New
Brenner et al., New York City, United States. In Radiat Res, 24 Aug 2015
Immunohistochemical analysis of γ H2AX foci formation in tissue sections revealed, compared to control tissue, proton-induced γ-H2AX foci formation in one of the two epidermal layers of the mouse ear.
Structural basis for retroviral integration into nucleosomes.
New
Impact
Cherepanov et al., Schwäbisch Hall, Germany. In Nature, 16 Aug 2015
Single-particle cryo-electron microscopy reveals a multivalent intasome-nucleosome interface involving both gyres of nucleosomal DNA and one H2A-H2B heterodimer.
Diversification of histone H2A variants during plant evolution.
Review
New
Berger et al., Vienna, Austria. In Trends Plant Sci, 31 Jul 2015
Nevertheless, in multicellular eukaryotes the two families, histone H2A and histone H3, have diversified significantly in key residues.
Checkpoint Activation of an Unconventional DNA Replication Program in Tetrahymena.
New
Kapler et al., College Station, United States. In Plos Genet, 31 Jul 2015
Chromosomes undergo global changes during HU-arrest, including phosphorylation of histone H2A.X, deacetylation of histone H3, and an apparent diminution in DNA content that can be blocked by the deacetylase inhibitor sodium butyrate.
Reconstitution of mitotic chromatids with a minimum set of purified factors.
New
Impact
Hirano et al., Wako, Japan. In Nat Cell Biol, 15 Jul 2015
We find that octameric nucleosomes containing the embryonic variant H2A.X-F are highly susceptible to FACT and function as the most productive substrate for subsequent actions of topo II and condensin I. Cdk1 phosphorylation of condensin I is the sole mitosis-specific modification required for chromatid reconstitution.
REV7 counteracts DNA double-strand break resection and affects PARP inhibition.
New
Impact
Rottenberg et al., Amsterdam, Netherlands. In Nature, 28 Jun 2015
REV7 is recruited to DSBs in a manner dependent on the H2AX-MDC1-RNF8-RNF168-53BP1 chromatin pathway, and seems to block HR and promote end joining in addition to its regulatory role in DNA damage tolerance.
Phase I Study of Single-Agent AZD1775 (MK-1775), a Wee1 Kinase Inhibitor, in Patients With Refractory Solid Tumors.
New
Impact
Kummar et al., Bethesda, United States. In J Clin Oncol, 11 Jun 2015
We conducted a phase I study of single-agent AZD1775 in adult patients with refractory solid tumors to determine its maximum-tolerated dose (MTD), pharmacokinetics, and modulation of phosphorylated Tyr15-Cdk (pY15-Cdk) and phosphorylated histone H2AX (γH2AX) levels in paired tumor biopsies.
Development of cancer-initiating cells and immortalized cells with genomic instability.
Review
New
Teraoka et al., Tokyo, Japan. In World J Stem Cells, Apr 2015
We summarize recently established knowledge of CIC development and cellular immortality, explore the role of the ARF/p53 module in protecting cells from transformation, and describe a risk factor for genomic destabilization that increases during the process of normal cell growth and differentiation and is associated with the downregulation of histone H2AX to levels representative of growth arrest in normal cells.
Phase I dose-escalation trial of checkpoint kinase 1 inhibitor MK-8776 as monotherapy and in combination with gemcitabine in patients with advanced solid tumors.
New
Impact
Munster et al., Santa Monica, United States. In J Clin Oncol, Apr 2015
Bioactivity was assessed by γ-H2AX ex vivo assay.
RNF20-SNF2H Pathway of Chromatin Relaxation in DNA Double-Strand Break Repair.
Review
New
Komatsu et al., Kyoto, Japan. In Genes (basel), Dec 2014
In DNA double-strand break (DSB) repair, the well-known mark of histones is the phosphorylation of the H2A variant, H2AX, which has been used as a surrogate marker of DSBs.
Interconnection between flowering time control and activation of systemic acquired resistance.
Review
New
Nandi et al., New Delhi, India. In Front Plant Sci, Dec 2014
Similarly, incorporation of histone variant H2A.Z in nucleosomes mediated by PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1, an ortholog of yeast chromatin remodeling complex SWR1, concomitantly influences SAR and flowering time.
The Structural Determinants behind the Epigenetic Role of Histone Variants.
Review
New
Ausió et al., Victoria, Canada. In Genes (basel), Dec 2014
A picture starts to emerge in which histone H2A variants, in addition to their individual specific contributions to the nucleosome structure and dynamics, globally impair the accessibility of linker histones to defined chromatin locations and may have important consequences for determining different states of chromatin metabolism.
L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation.
New
Kania et al., Łódź, Poland. In Cell Commun Signal, Dec 2014
The lactate-mediated stimulation of DNA repair and cellular resistance to neocarzinostatin, doxorubicin and cisplatin were studied using γ-H2AX, comet and clonogenic assays.
Clinical significance of increased expression of Nijmegen breakage syndrome gene (NBS1) in human primary liver cancer.
New
Huang et al., Beijing, China. In Hepatol Int, Apr 2014
The percentage of Ki-67 antigen-positive cells and the level of phosphorylated histone H2AX (γ-H2AX) were detected to evaluate the relationship of Nbs1 expression with proliferation and the degree of DNA damage in HCC cells.
RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling.
Impact
GeneRIF
Sixma et al., Amsterdam, Netherlands. In Cell, 2012
Ubiquitin-dependent signaling during the DNA damage response (DDR) to double-strand breaks is initiated by two E3 ligases, RNF8 and RNF168, targeting histone H2A and H2AX. Study shows that ubiquitin chains per se are insufficient for signaling, but RNF168 target ubiquitination is required for DDR damage.
Redundant and nonredundant functions of ATM and H2AX in αβ T-lineage lymphocytes.
GeneRIF
Bassing et al., Philadelphia, United States. In J Immunol, 2012
Both redundant and nonredundant functions of H2AX and ATM transgenes are required for normal recombination of T cell receptor (TCR) loci, proliferative expansion of developing thymocytes, and maintenance of genomic stability in cycling alphabeta T-lineage cells.
High basal γH2AX levels sustain self-renewal of mouse embryonic and induced pluripotent stem cells.
GeneRIF
Giachino et al., Torino, Italy. In Stem Cells, 2012
Findings suggest a novel function of H2AX that expands the knowledge of this histone variant beyond its role in DNA damage and into a new specialized biological function in mouse pluripotent stem cells.
Deregulation of BRCA1 leads to impaired spatiotemporal dynamics of γ-H2AX and DNA damage responses in Huntington's disease.
GeneRIF
Ryu et al., Seoul, South Korea. In Mol Neurobiol, 2012
BRCA1 directly modulates the spatiotemporal dynamics of gamma-H2AX upon genotoxic stress and serves as a molecular maker for neuronal DNA damage response in Huntington's disease.
Loss of CCDC6, the first identified RET partner gene, affects pH2AX S139 levels and accelerates mitotic entry upon DNA damage.
GeneRIF
Celetti et al., Napoli, Italy. In Plos One, 2011
Following genotoxic stress, loss or inactivation of CCDC6 in cancers that carry the CCDC6 fusion, accelerates the dephosphorylation of pH2AX S139, resulting in defective G2 arrest and premature mitotic entry.
More papers using H2A antibodies
Complementary functions of ATM and H2AX in development and suppression of genomic instability
Supplier
Malle Ernst et al., In DNA Repair, 2007
... , termed γ-H2AX, Cell Signaling Technology, Beverly, MA, USA); ...
Enhanced inhibition of adipogenesis and induction of apoptosis in 3T3-L1 adipocytes with combinations of resveratrol and quercetin
Supplier
Cook John A. et al., In Frontiers in Oncology, 2007
... CA, USA); mouse monoclonal anti-phospho histone H2AX (Ser139), clone JBW301, and rabbit antiserum to histone H2A (acidic patch) were from Upstate Cell Signaling Solutions (Temecula, CA, USA) ...
Integrin-mediated transforming growth factor-beta activation regulates homeostasis of the pulmonary epithelial-mesenchymal trophic unit
Supplier
Kuwano Kazuyoshi et al., In Oncoimmunology, 2005
... used were rabbit anti-LC3 (Novus), rabbit anti-ATG5 (Novus), mouse anti-p21 (Cell signaling Technology), rabbit anti-phospho-histone H2A.X (Ser139) (Cell signaling Technology), mouse anti-poly and ...
Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster.
Supplier
Oshima Robert, In PLoS ONE, 2005
... Cruz Biotechnology), Ki67 (Lab Vision, Fremont, CA, USA); mouse monoclonal antibodies for phosphor-H2AX, Ser 139, (γH2AX) (BioLegend, San Diego, CA, USA), ...
The comet assay: a method to measure DNA damage in individual cells
Supplier
Fritz G et al., In Cell Death & Disease, 2005
... H2AX anti-rabbit antibody (pS139) (Epitomics, Burlingame, CA, USA), ERK2 ...
Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair.
Supplier
Kirkpatrick David T., In PLoS ONE, 2004
... anti-CHK2 (Santa Cruz), anti-phospho-CHK1 (Ser 345) (Cell signaling), anti-phospho-CHK2 (Thr 68) (Cell signaling), anti-γH2AX (Upstate), anti-H2AX (Abcam), anti-HA (HA-7, Sigma) ...
Histochemical localization of alkaline phosphatase activity in decalcified bone and cartilage
Supplier
Tang Liping et al., In Scientific Reports, 2001
... Primary antibodies CD34, CD44, CD105, CD29, SCA-1, CD45, osteocalcin, 53BP1, γ-H2AX, were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA) ...
Functional interaction between BLM helicase and 53BP1 in a Chk1-mediated pathway during S-phase arrest
Supplier
Harris Curtis C. et al., In The Journal of Cell Biology, 2001
... Anti-γ-H2AX: rabbit polyclonal PC-100 (Trevigen).
Crystal structure of a nucleosome core particle containing the variant histone H2A.Z
Supplier
Chrivia John C. et al., In International Journal of Cell Biology, 1999
... Other commercial antibodies were histone H2A.Z polyclonal (Abcam, ab4174), histone H3 polyclonal ...
A peptidomimetic inhibitor of farnesyl:protein transferase blocks the anchorage-dependent and -independent growth of human tumor cell lines
Supplier
Nie Daotai et al., In Cancer Cell International, 1994
... from Clontech; Lamin A/C (sc-20681) and emerin antibodies were purchased from Santa Cruz biotechnology; anti-phosphorylated histone-H2AX polyclonal antibody was from Trevigen.
Methods for clonal growth and serial cultivation of normal human epidermal keratinocytes and mesothelial cells.
Supplier
Karl Mike O., In PLoS ONE, 1988
... (Abcam), anti Keratin 1,10,11 (Ks 8.60; Abcam), anti Keratin 10 (RKSE60; Abcam), anti-keratin 1 (AF87; Covance), anti-γH2AX (Ser139; Millipore), anti-BrdU (B44; BD Biosciences), anti-phospho-histone H3 (P-H3; Upstate ...
Changes in the cell kinetics of pig epidermis after single doses of X rays.
Supplier
Borgmann Kerstin, In PLoS ONE, 1987
... Mouse anti-γ-H2AX, JBW3011/500Milipore ...
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