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H2A histone family, member X

H2A, H2AX
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: Histone, CAN, Atm, p53, V1a
Papers using H2A antibodies
Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by silica nanomaterials in human neuronal cell line
Supplier
Celotti Lucia et al., In Analytical and Bioanalytical Chemistry, 2009
... Anti-γ-H2AX mouse monoclonal antibody was purchased from Upstate, Millipore (Billerica, MA, USA) and from Molecular Probes (Alexa Fluor, Life Technologies, Carlsbad, CA, USA) ...
Prediction and functional analysis of native disorder in proteins from the three kingdoms of life.
Supplier
Lustig Arthur J., In PLoS ONE, 2009
... Human H2A peptides 1–100 (ab27894) and 100-end (ab15660) were from Abcam.
Parameters of oligonucleotide-mediated gene modification in mouse ES cells
Supplier
Kmiec Eric B et al., In Molecular therapy. Nucleic acids, 2009
... antibodies were added at a dilution of 1:100 in 10% milk and kept at 4 °C overnight (γH2AX: Cell Signaling, Boston, MA; PCNA: Santa ...
Aggresome-forming TTRAP mediates pro-apoptotic properties of Parkinson's disease-associated DJ-1 missense mutations
Supplier
Li R et al., In Oncogene, 2008
... (Danvers, MA, USA); phospho-JNK1/2/3(sc-6254), MYC(sc-764) and γ-H2AX(sc-101696) from Santa Cruz Biotechnology, Inc ...
Noxa mediates bortezomib induced apoptosis in both sensitive and intrinsically resistant mantle cell lymphoma cells and this effect is independent of constitutive activity of the AKT and NF-kappaB pathways.
Supplier
Hofmann Andreas, In PLoS ONE, 2007
... out using the following antibodies: FLAG antibody (Sigma), NOXA antibody (EMD), ATF3 (Santa Cruz), histone H2A (Abcam).
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Papers on H2A
Mechanism of the inhibition of leukemia cell growth and induction of apoptosis through the activation of ATR and PTEN by the topoisomerase inhibitor 3EZ, 20Ac-ingenol.
Review
New
Sawada et al., Tokyo, Japan. In Leuk Res, 30 Sep 2015
In further experiments, p53 protein expression was increased, and H2AX phosphorylation and p21 protein expression were induced after treatment with 3EZ, 20Ac-ingenol.
Replication-induced DNA damage after PARP inhibition causes G2 delay, and cell line-dependent apoptosis, necrosis and multinucleation.
New
Stokke et al., Oslo, Norway. In Cell Cycle, 27 Sep 2015
PARP inhibition induced a severe growth inhibition in this cell line panel and increased the levels of phosphorylated H2AX-associated DNA damage in S phase.
Mean frequency and relative fluorescence intensity measurement of γ-H2AX foci dose response in PBL exposed to γ-irradiation: An inter- and intra-laboratory comparison and its relevance for radiation triage.
New
Venkatachalam et al., Chennai, India. In Cytometry A, 25 Sep 2015
UNASSIGNED: Measurement of γ-H2AX protein changes in the peripheral blood lymphocytes (PBL) of individuals exposed to ionizing radiation is a simple, sensitive, and rapid assay for radiation triage and early marker of dose estimation.
Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation.
New
Impact
Lu et al., Houston, United States. In Nat Cell Biol, 03 Sep 2015
Here, we show that exposure to ionizing radiation induces DNA-PK-dependent phosphorylation of nuclear fumarase at Thr 236, which leads to an interaction between fumarase and the histone variant H2A.Z at DNA double-strand break (DSB) regions.
Reconstitution of mitotic chromatids with a minimum set of purified factors.
New
Impact
Hirano et al., Wako, Japan. In Nat Cell Biol, 31 Aug 2015
We find that octameric nucleosomes containing the embryonic variant H2A.X-F are highly susceptible to FACT and function as the most productive substrate for subsequent actions of topo II and condensin I. Cdk1 phosphorylation of condensin I is the sole mitosis-specific modification required for chromatid reconstitution.
Structural basis for retroviral integration into nucleosomes.
New
Impact
Cherepanov et al., Schwäbisch Hall, Germany. In Nature, 16 Aug 2015
Single-particle cryo-electron microscopy reveals a multivalent intasome-nucleosome interface involving both gyres of nucleosomal DNA and one H2A-H2B heterodimer.
Diversification of histone H2A variants during plant evolution.
Review
New
Berger et al., Vienna, Austria. In Trends Plant Sci, 31 Jul 2015
Nevertheless, in multicellular eukaryotes the two families, histone H2A and histone H3, have diversified significantly in key residues.
REV7 counteracts DNA double-strand break resection and affects PARP inhibition.
New
Impact
Rottenberg et al., Amsterdam, Netherlands. In Nature, Jun 2015
REV7 is recruited to DSBs in a manner dependent on the H2AX-MDC1-RNF8-RNF168-53BP1 chromatin pathway, and seems to block HR and promote end joining in addition to its regulatory role in DNA damage tolerance.
Phase I Study of Single-Agent AZD1775 (MK-1775), a Wee1 Kinase Inhibitor, in Patients With Refractory Solid Tumors.
New
Impact
Kummar et al., Bethesda, United States. In J Clin Oncol, Jun 2015
We conducted a phase I study of single-agent AZD1775 in adult patients with refractory solid tumors to determine its maximum-tolerated dose (MTD), pharmacokinetics, and modulation of phosphorylated Tyr15-Cdk (pY15-Cdk) and phosphorylated histone H2AX (γH2AX) levels in paired tumor biopsies.
Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation.
New
Davis et al., Oxford, United Kingdom. In Nat Commun, Dec 2014
Here, we generate synthetic, pure GlcNAcylated histones and nucleosomes and reveal that GlcNAcylation can modulate structure through direct destabilization of H2A/H2B dimers in the nucleosome, thus promoting an 'open' chromatin state.
The Structural Determinants behind the Epigenetic Role of Histone Variants.
Review
New
Ausió et al., Victoria, Canada. In Genes (basel), Dec 2014
A picture starts to emerge in which histone H2A variants, in addition to their individual specific contributions to the nucleosome structure and dynamics, globally impair the accessibility of linker histones to defined chromatin locations and may have important consequences for determining different states of chromatin metabolism.
RNF20-SNF2H Pathway of Chromatin Relaxation in DNA Double-Strand Break Repair.
Review
New
Komatsu et al., Kyoto, Japan. In Genes (basel), Dec 2014
In DNA double-strand break (DSB) repair, the well-known mark of histones is the phosphorylation of the H2A variant, H2AX, which has been used as a surrogate marker of DSBs.
Chromatin Dynamics in Vivo: A Game of Musical Chairs.
Review
New
Dalal et al., Bethesda, United States. In Genes (basel), Dec 2014
In this review, we will focus on the localization of the major histone variants H3.3, CENP-A, H2A.Z, and macroH2A, as well as how these variants have evolved, their structural differences, and their functional significance in vivo.
Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines.
New
Chen et al., Wuhan, China. In Int J Clin Exp Med, Dec 2014
DNA damage of glioblastoma cells caused by puerarin exposure was evaluated by γ-H2AX foci detection, and the expressions of p-AKT, caspase-3 and apoptosis-related proteins were detected by Western blotting after puerarin treatment.
Phytometabolite Dehydroleucodine Induces Cell Cycle Arrest, Apoptosis, and DNA Damage in Human Astrocytoma Cells through p73/p53 Regulation.
New
Ostrosky-Wegman et al., Mexico. In Plos One, Dec 2014
A marked induction of the levels of total TP73 and phosphorylated TP53, TP73, and γ-H2AX proteins was observed in D384 cells exposed to DhL, but no increase in total TP53 levels was detected.
RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling.
Impact
GeneRIF
Sixma et al., Amsterdam, Netherlands. In Cell, 2012
Ubiquitin-dependent signaling during the DNA damage response (DDR) to double-strand breaks is initiated by two E3 ligases, RNF8 and RNF168, targeting histone H2A and H2AX. Study shows that ubiquitin chains per se are insufficient for signaling, but RNF168 target ubiquitination is required for DDR damage.
Redundant and nonredundant functions of ATM and H2AX in αβ T-lineage lymphocytes.
GeneRIF
Bassing et al., Philadelphia, United States. In J Immunol, 2012
Both redundant and nonredundant functions of H2AX and ATM transgenes are required for normal recombination of T cell receptor (TCR) loci, proliferative expansion of developing thymocytes, and maintenance of genomic stability in cycling alphabeta T-lineage cells.
High basal γH2AX levels sustain self-renewal of mouse embryonic and induced pluripotent stem cells.
GeneRIF
Giachino et al., Torino, Italy. In Stem Cells, 2012
Findings suggest a novel function of H2AX that expands the knowledge of this histone variant beyond its role in DNA damage and into a new specialized biological function in mouse pluripotent stem cells.
Deregulation of BRCA1 leads to impaired spatiotemporal dynamics of γ-H2AX and DNA damage responses in Huntington's disease.
GeneRIF
Ryu et al., Seoul, South Korea. In Mol Neurobiol, 2012
BRCA1 directly modulates the spatiotemporal dynamics of gamma-H2AX upon genotoxic stress and serves as a molecular maker for neuronal DNA damage response in Huntington's disease.
Loss of CCDC6, the first identified RET partner gene, affects pH2AX S139 levels and accelerates mitotic entry upon DNA damage.
GeneRIF
Celetti et al., Napoli, Italy. In Plos One, 2011
Following genotoxic stress, loss or inactivation of CCDC6 in cancers that carry the CCDC6 fusion, accelerates the dephosphorylation of pH2AX S139, resulting in defective G2 arrest and premature mitotic entry.
More papers using H2A antibodies
Complementary functions of ATM and H2AX in development and suppression of genomic instability
Supplier
Malle Ernst et al., In DNA Repair, 2007
... , termed γ-H2AX, Cell Signaling Technology, Beverly, MA, USA); ...
Enhanced inhibition of adipogenesis and induction of apoptosis in 3T3-L1 adipocytes with combinations of resveratrol and quercetin
Supplier
Cook John A. et al., In Frontiers in Oncology, 2007
... CA, USA); mouse monoclonal anti-phospho histone H2AX (Ser139), clone JBW301, and rabbit antiserum to histone H2A (acidic patch) were from Upstate Cell Signaling Solutions (Temecula, CA, USA) ...
Integrin-mediated transforming growth factor-beta activation regulates homeostasis of the pulmonary epithelial-mesenchymal trophic unit
Supplier
Kuwano Kazuyoshi et al., In Oncoimmunology, 2005
... used were rabbit anti-LC3 (Novus), rabbit anti-ATG5 (Novus), mouse anti-p21 (Cell signaling Technology), rabbit anti-phospho-histone H2A.X (Ser139) (Cell signaling Technology), mouse anti-poly and ...
Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster.
Supplier
Oshima Robert, In PLoS ONE, 2005
... Cruz Biotechnology), Ki67 (Lab Vision, Fremont, CA, USA); mouse monoclonal antibodies for phosphor-H2AX, Ser 139, (γH2AX) (BioLegend, San Diego, CA, USA), ...
The comet assay: a method to measure DNA damage in individual cells
Supplier
Fritz G et al., In Cell Death & Disease, 2005
... H2AX anti-rabbit antibody (pS139) (Epitomics, Burlingame, CA, USA), ERK2 ...
Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair.
Supplier
Kirkpatrick David T., In PLoS ONE, 2004
... anti-CHK2 (Santa Cruz), anti-phospho-CHK1 (Ser 345) (Cell signaling), anti-phospho-CHK2 (Thr 68) (Cell signaling), anti-γH2AX (Upstate), anti-H2AX (Abcam), anti-HA (HA-7, Sigma) ...
Histochemical localization of alkaline phosphatase activity in decalcified bone and cartilage
Supplier
Tang Liping et al., In Scientific Reports, 2001
... Primary antibodies CD34, CD44, CD105, CD29, SCA-1, CD45, osteocalcin, 53BP1, γ-H2AX, were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA) ...
Functional interaction between BLM helicase and 53BP1 in a Chk1-mediated pathway during S-phase arrest
Supplier
Harris Curtis C. et al., In The Journal of Cell Biology, 2001
... Anti-γ-H2AX: rabbit polyclonal PC-100 (Trevigen).
Crystal structure of a nucleosome core particle containing the variant histone H2A.Z
Supplier
Chrivia John C. et al., In International Journal of Cell Biology, 1999
... Other commercial antibodies were histone H2A.Z polyclonal (Abcam, ab4174), histone H3 polyclonal ...
A peptidomimetic inhibitor of farnesyl:protein transferase blocks the anchorage-dependent and -independent growth of human tumor cell lines
Supplier
Nie Daotai et al., In Cancer Cell International, 1994
... from Clontech; Lamin A/C (sc-20681) and emerin antibodies were purchased from Santa Cruz biotechnology; anti-phosphorylated histone-H2AX polyclonal antibody was from Trevigen.
Methods for clonal growth and serial cultivation of normal human epidermal keratinocytes and mesothelial cells.
Supplier
Karl Mike O., In PLoS ONE, 1988
... (Abcam), anti Keratin 1,10,11 (Ks 8.60; Abcam), anti Keratin 10 (RKSE60; Abcam), anti-keratin 1 (AF87; Covance), anti-γH2AX (Ser139; Millipore), anti-BrdU (B44; BD Biosciences), anti-phospho-histone H3 (P-H3; Upstate ...
Changes in the cell kinetics of pig epidermis after single doses of X rays.
Supplier
Borgmann Kerstin, In PLoS ONE, 1987
... Mouse anti-γ-H2AX, JBW3011/500Milipore ...
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