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H2A histone family, member X

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: Histone, CAN, p53, Atm, V1a
Papers using H2A antibodies
Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by silica nanomaterials in human neuronal cell line
Celotti Lucia et al., In Analytical and Bioanalytical Chemistry, 2009
... Anti-γ-H2AX mouse monoclonal antibody was purchased from Upstate, Millipore (Billerica, MA, USA) and from Molecular Probes (Alexa Fluor, Life Technologies, Carlsbad, CA, USA) ...
Prediction and functional analysis of native disorder in proteins from the three kingdoms of life.
Lustig Arthur J., In PLoS ONE, 2009
... Human H2A peptides 1–100 (ab27894) and 100-end (ab15660) were from Abcam.
Parameters of oligonucleotide-mediated gene modification in mouse ES cells
Kmiec Eric B et al., In Molecular therapy. Nucleic acids, 2009
... antibodies were added at a dilution of 1:100 in 10% milk and kept at 4 °C overnight (γH2AX: Cell Signaling, Boston, MA; PCNA: Santa ...
Aggresome-forming TTRAP mediates pro-apoptotic properties of Parkinson's disease-associated DJ-1 missense mutations
Li R et al., In Oncogene, 2008
... (Danvers, MA, USA); phospho-JNK1/2/3(sc-6254), MYC(sc-764) and γ-H2AX(sc-101696) from Santa Cruz Biotechnology, Inc ...
Noxa mediates bortezomib induced apoptosis in both sensitive and intrinsically resistant mantle cell lymphoma cells and this effect is independent of constitutive activity of the AKT and NF-kappaB pathways.
Hofmann Andreas, In PLoS ONE, 2007
... out using the following antibodies: FLAG antibody (Sigma), NOXA antibody (EMD), ATF3 (Santa Cruz), histone H2A (Abcam).
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Papers on H2A
PTH1-34 Blocks Radiation-Induced Osteoblast Apoptosis by Enhancing DNA Repair through Canonical Wnt Pathway.
Qin et al., United States. In J Biol Chem, 21 Nov 2014
γ-H2AX foci staining and comet assay revealed that PTH efficiently promotes the repair of DNA double strand breaks (DSBs) in irradiated osteoblasts via activating the β-catenin pathway.
Unusual Dispersion of Histone Repeats on the Whole Chromosomal Complement and Their Colocalization with Ribosomal Genes in Rachycentron canadum (Rachycentridae, Perciformes).
Molina et al., Natal, Brazil. In Cytogenet Genome Res, 17 Nov 2014
A FISH analysis was performed using 4 multigene families as probes, represented by 5S and 18S ribosomal genes and histones H2B-H2A and H3.
Downregulation of BRCA1-BRCA2-containing complex subunit 3 sensitizes glioma cells to temozolomide.
Tzeng et al., Tainan City, Taiwan. In Oncotarget, 07 Nov 2014
The increase in phosphorylated H2AX foci (γH2AX) formation, an indicator of DNA damage, persisted in TMZ-treated glioma cells with stable knockdown BRCC3 expression, suggesting that BRCC3 gene deficiency is associated with DNA repair impairment.
Tyrosine phosphorylation of histone H2A by CK2 regulates transcriptional elongation.
Rosenfeld et al., San Diego, United States. In Nature, 24 Oct 2014
This regulation is based on the phosphorylation of a highly conserved tyrosine residue, Tyr 57, in histone H2A and is mediated by the unsuspected tyrosine kinase activity of casein kinase 2 (CK2).
Histone H2A.Z subunit exchange controls consolidation of recent and remote memory.
Sweatt et al., Birmingham, United States. In Nature, 14 Oct 2014
Here we show that histone H2A.Z, a variant of histone H2A, is actively exchanged in response to fear conditioning in the hippocampus and the cortex, where it mediates gene expression and restrains the formation of recent and remote memory.
The developmental potential of iPSCs is greatly influenced by reprogramming factor selection.
Jaenisch et al., Jerusalem, Israel. In Cell Stem Cell, 04 Oct 2014
Although differentially methylated regions, transcript number of master regulators, establishment of specific superenhancers, and global aneuploidy were comparable between high- and low-quality lines, aberrant gene expression, trisomy of chromosome 8, and abnormal H2A.X deposition were distinguishing features that could potentially also be applicable to human.
Quality control: H2A.X links to better iPSCs.
Gao et al., Shanghai, China. In Cell Stem Cell, 04 Oct 2014
Two papers in this issue of Cell Stem Cell (Buganim et al., 2014; Wu et al., 2014) now show that H2A.X deposition can serve as a functional epigenetic marker for distinguishing the quality of iPSCs, thus providing insights for better reprogramming methods.
Histone variant H2A.X deposition pattern serves as a functional epigenetic mark for distinguishing the developmental potentials of iPSCs.
Xiao et al., New Haven, United States. In Cell Stem Cell, 04 Oct 2014
Here we show that the histone variant H2A.X is a functional marker that can distinguish the developmental potentials of mouse iPSC lines.
[Influence of Calcineurin on Apoptosis of pre-B Lymphocytes and the Leukemia Cells Derived from pre-B lymphocytes].
Xiang et al., Zhangjiakou, China. In Zhongguo Shi Yan Xue Ye Xue Za Zhi, 30 Sep 2014
The quantitative real-time PCR was used to detect the differential expressions of H2AX-associated phosphakinase ATM, ATR, DNA-PKs, JNK1, P38 and the γ-H2AX-related phosphatase PP1, PP2A, calcineurin, PP4, PP6, PP5 between S9 and S4C2 cell lines.
Environmental responses mediated by histone variants.
Henikoff et al., Seattle, United States. In Trends Cell Biol, 20 Sep 2014
Phosphorylation of histone H2A.X rapidly marks double-strand DNA breaks for chromatin repair, which is mediated by both H2A and H3 histone variants.
γ-H2AX as a biomarker for DNA double-strand breaks in ecotoxicology.
Garaj-Vrhovac et al., Zagreb, Croatia. In Ecotoxicol Environ Saf, Jul 2014
Soon after the occurrence of a DNA double-strand break (DSB), the formation of γ-H2AX histone variants is to be expected.
H2A-DUBbing the mammalian epigenome: expanding frontiers for histone H2A deubiquitinating enzymes in cell biology and physiology.
Nijnik et al., Canada. In Int J Biochem Cell Biol, May 2014
Posttranslational modifications of histone H2A through the attachment of ubiquitin or poly-ubiquitin conjugates are common in mammalian genomes and play an important role in the regulation of chromatin structure, gene expression, and DNA repair.
Dynamic regulation and function of histone monoubiquitination in plants.
Shen et al., Strasbourg, France. In Front Plant Sci, Dec 2013
While H2A monoubiquitination (H2Aub1) is generally associated with transcription repression mediated by the Polycomb pathway, H2Bub1 is involved in transcription activation.
Distinct roles for histone chaperones in the deposition of Htz1 in chromatin.
Wan et al., Nanjing, China. In Biosci Rep, Dec 2013
Histone variant Htz1 substitution for H2A plays important roles in diverse DNA transactions.
Histone exchange and histone modifications during transcription and aging.
Tyler et al., Houston, United States. In Biochim Biophys Acta, Mar 2013
The basic repeating unit of chromatin is the nucleosome, which includes 147 bp of DNA wrapped 1.65 times around an octamer of core histone proteins comprising two molecules each of H2A, H2B, H3 and H4.
RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling.
Sixma et al., Amsterdam, Netherlands. In Cell, 2012
Ubiquitin-dependent signaling during the DNA damage response (DDR) to double-strand breaks is initiated by two E3 ligases, RNF8 and RNF168, targeting histone H2A and H2AX. Study shows that ubiquitin chains per se are insufficient for signaling, but RNF168 target ubiquitination is required for DDR damage.
Redundant and nonredundant functions of ATM and H2AX in αβ T-lineage lymphocytes.
Bassing et al., Philadelphia, United States. In J Immunol, 2012
Both redundant and nonredundant functions of H2AX and ATM transgenes are required for normal recombination of T cell receptor (TCR) loci, proliferative expansion of developing thymocytes, and maintenance of genomic stability in cycling alphabeta T-lineage cells.
High basal γH2AX levels sustain self-renewal of mouse embryonic and induced pluripotent stem cells.
Giachino et al., Torino, Italy. In Stem Cells, 2012
Findings suggest a novel function of H2AX that expands the knowledge of this histone variant beyond its role in DNA damage and into a new specialized biological function in mouse pluripotent stem cells.
Deregulation of BRCA1 leads to impaired spatiotemporal dynamics of γ-H2AX and DNA damage responses in Huntington's disease.
Ryu et al., Seoul, South Korea. In Mol Neurobiol, 2012
BRCA1 directly modulates the spatiotemporal dynamics of gamma-H2AX upon genotoxic stress and serves as a molecular maker for neuronal DNA damage response in Huntington's disease.
Loss of CCDC6, the first identified RET partner gene, affects pH2AX S139 levels and accelerates mitotic entry upon DNA damage.
Celetti et al., Napoli, Italy. In Plos One, 2011
Following genotoxic stress, loss or inactivation of CCDC6 in cancers that carry the CCDC6 fusion, accelerates the dephosphorylation of pH2AX S139, resulting in defective G2 arrest and premature mitotic entry.
More papers using H2A antibodies
Complementary functions of ATM and H2AX in development and suppression of genomic instability
Malle Ernst et al., In DNA Repair, 2007
... , termed γ-H2AX, Cell Signaling Technology, Beverly, MA, USA); ...
Enhanced inhibition of adipogenesis and induction of apoptosis in 3T3-L1 adipocytes with combinations of resveratrol and quercetin
Cook John A. et al., In Frontiers in Oncology, 2007
... CA, USA); mouse monoclonal anti-phospho histone H2AX (Ser139), clone JBW301, and rabbit antiserum to histone H2A (acidic patch) were from Upstate Cell Signaling Solutions (Temecula, CA, USA) ...
Integrin-mediated transforming growth factor-beta activation regulates homeostasis of the pulmonary epithelial-mesenchymal trophic unit
Kuwano Kazuyoshi et al., In Oncoimmunology, 2005
... used were rabbit anti-LC3 (Novus), rabbit anti-ATG5 (Novus), mouse anti-p21 (Cell signaling Technology), rabbit anti-phospho-histone H2A.X (Ser139) (Cell signaling Technology), mouse anti-poly and ...
Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster.
Oshima Robert, In PLoS ONE, 2005
... Cruz Biotechnology), Ki67 (Lab Vision, Fremont, CA, USA); mouse monoclonal antibodies for phosphor-H2AX, Ser 139, (γH2AX) (BioLegend, San Diego, CA, USA), ...
The comet assay: a method to measure DNA damage in individual cells
Fritz G et al., In Cell Death & Disease, 2005
... H2AX anti-rabbit antibody (pS139) (Epitomics, Burlingame, CA, USA), ERK2 ...
Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair.
Kirkpatrick David T., In PLoS ONE, 2004
... anti-CHK2 (Santa Cruz), anti-phospho-CHK1 (Ser 345) (Cell signaling), anti-phospho-CHK2 (Thr 68) (Cell signaling), anti-γH2AX (Upstate), anti-H2AX (Abcam), anti-HA (HA-7, Sigma) ...
Histochemical localization of alkaline phosphatase activity in decalcified bone and cartilage
Tang Liping et al., In Scientific Reports, 2001
... Primary antibodies CD34, CD44, CD105, CD29, SCA-1, CD45, osteocalcin, 53BP1, γ-H2AX, were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA) ...
Functional interaction between BLM helicase and 53BP1 in a Chk1-mediated pathway during S-phase arrest
Harris Curtis C. et al., In The Journal of Cell Biology, 2001
... Anti-γ-H2AX: rabbit polyclonal PC-100 (Trevigen).
Crystal structure of a nucleosome core particle containing the variant histone H2A.Z
Chrivia John C. et al., In International Journal of Cell Biology, 1999
... Other commercial antibodies were histone H2A.Z polyclonal (Abcam, ab4174), histone H3 polyclonal ...
A peptidomimetic inhibitor of farnesyl:protein transferase blocks the anchorage-dependent and -independent growth of human tumor cell lines
Nie Daotai et al., In Cancer Cell International, 1994
... from Clontech; Lamin A/C (sc-20681) and emerin antibodies were purchased from Santa Cruz biotechnology; anti-phosphorylated histone-H2AX polyclonal antibody was from Trevigen.
Methods for clonal growth and serial cultivation of normal human epidermal keratinocytes and mesothelial cells.
Karl Mike O., In PLoS ONE, 1988
... (Abcam), anti Keratin 1,10,11 (Ks 8.60; Abcam), anti Keratin 10 (RKSE60; Abcam), anti-keratin 1 (AF87; Covance), anti-γH2AX (Ser139; Millipore), anti-BrdU (B44; BD Biosciences), anti-phospho-histone H3 (P-H3; Upstate ...
Changes in the cell kinetics of pig epidermis after single doses of X rays.
Borgmann Kerstin, In PLoS ONE, 1987
... Mouse anti-γ-H2AX, JBW3011/500Milipore ...
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