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H2A histone family, member X

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: Histone, CAN, Atm, p53, V1a
Papers using H2A antibodies
Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by silica nanomaterials in human neuronal cell line
Celotti Lucia et al., In Analytical and Bioanalytical Chemistry, 2009
... Anti-γ-H2AX mouse monoclonal antibody was purchased from Upstate, Millipore (Billerica, MA, USA) and from Molecular Probes (Alexa Fluor, Life Technologies, Carlsbad, CA, USA) ...
Prediction and functional analysis of native disorder in proteins from the three kingdoms of life.
Lustig Arthur J., In PLoS ONE, 2009
... Human H2A peptides 1–100 (ab27894) and 100-end (ab15660) were from Abcam.
Parameters of oligonucleotide-mediated gene modification in mouse ES cells
Kmiec Eric B et al., In Molecular therapy. Nucleic acids, 2009
... antibodies were added at a dilution of 1:100 in 10% milk and kept at 4 °C overnight (γH2AX: Cell Signaling, Boston, MA; PCNA: Santa ...
Aggresome-forming TTRAP mediates pro-apoptotic properties of Parkinson's disease-associated DJ-1 missense mutations
Li R et al., In Oncogene, 2008
... (Danvers, MA, USA); phospho-JNK1/2/3(sc-6254), MYC(sc-764) and γ-H2AX(sc-101696) from Santa Cruz Biotechnology, Inc ...
Noxa mediates bortezomib induced apoptosis in both sensitive and intrinsically resistant mantle cell lymphoma cells and this effect is independent of constitutive activity of the AKT and NF-kappaB pathways.
Hofmann Andreas, In PLoS ONE, 2007
... out using the following antibodies: FLAG antibody (Sigma), NOXA antibody (EMD), ATF3 (Santa Cruz), histone H2A (Abcam).
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Papers on H2A
Cellular Recognition and Repair of Monofunctional-Intercalative Platinum-DNA Adducts.
Bierbach et al., In Chem Res Toxicol, 12 Nov 2015
At the same dose, P1-A1, but not cisplatin, elicited a distinct requirement for DNA double-strand break repair and stalled replication fork repair, which caused nuclear fluorescent staining related to high levels of MUS81, a specialized repair endonuclease, and phosphorylated histone protein gamma-H2AX.
Preclinical activity of the novel BMI-1 inhibitor PTC-209 in acute myeloid leukemia: implications for leukemia therapy.
Kojima et al., Saga, Japan. In Cancer Sci, 09 Nov 2015
Treatment with PTC-209 reduced protein level of BMI-1 and its downstream target mono-ubiquitinated histone H2A and triggered several molecular events consistent with the induction of apoptosis, i.e., loss of mitochondrial membrane potential, caspase-3 cleavage, BAX activation and phosphatidylserine externalization.
Interaction of the Jhd2 H3K4 demethylase with chromatin is controlled by histone H2A surfaces and restricted by H2B ubiquitination.
Chandrasekharan et al., United States. In J Biol Chem, 08 Nov 2015
We report a novel interaction between the Jhd2 PHD finger and histone H2A.
Cytotoxic and genotoxic effects mediated by M2 muscarinic receptor activation in human glioblastoma cells.
Tata et al., Roma, Italy. In Neurochem Int, 06 Nov 2015
Although there was an up-regulation of some detoxifying enzyme expression such as superoxide dismutase (MnSOD) and sirtuin-1 (SIRT1), the cytotoxic effect caused by arecaidine treatment caused DNA damage, as demonstrated by the increase of histone γ-H2AX positive cells, and chromosomal aberrations.
FACT Disrupts Nucleosome Structure by Binding H2A-H2B with Conserved Peptide Motifs.
Hill et al., Salt Lake City, United States. In Mol Cell, 06 Nov 2015
Moreover, unexpected similarity to binding of ANP32E and Swr1 with H2A.Z-H2B reveals that diverse H2A-H2B chaperones use common mechanisms of histone binding and regulating nucleosome functions.
Loss of BAP1 function leads to EZH2-dependent transformation.
Levine et al., New York City, United States. In Nat Med, 05 Nov 2015
UNASSIGNED: The tumor suppressors BAP1 and ASXL1 interact to form a polycomb deubiquitinase complex that removes monoubiquitin from histone H2A lysine 119 (H2AK119Ub).
DNA Damage Response Assessments in Human Tumor Samples Provide Functional Biomarkers of Radiosensitivity.
von Neubeck et al., Heidelberg, Germany. In Semin Radiat Oncol, 31 Oct 2015
Investigators are increasingly studying the subnuclear accumulation (ie, foci) of proteins in the DNA damage response (DDR), such as gamma-H2AX, 53BP1, or RAD51, as a surrogate of treatment sensitivity.
Extensive Nuclear Reprogramming Underlies Lineage Conversion into Functional Trophoblast Stem-like Cells.
Buganim et al., Jerusalem, Israel. In Cell Stem Cell, 22 Oct 2015
iTSCs possess transcriptional profiles highly similar to blastocyst-derived TSCs, with comparable methylation and H3K27ac patterns and genome-wide H2A.X deposition.
Mechanism of the inhibition of leukemia cell growth and induction of apoptosis through the activation of ATR and PTEN by the topoisomerase inhibitor 3EZ, 20Ac-ingenol.
Sawada et al., Tokyo, Japan. In Leuk Res, 30 Sep 2015
In further experiments, p53 protein expression was increased, and H2AX phosphorylation and p21 protein expression were induced after treatment with 3EZ, 20Ac-ingenol.
Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation.
Lu et al., Houston, United States. In Nat Cell Biol, 30 Sep 2015
Here, we show that exposure to ionizing radiation induces DNA-PK-dependent phosphorylation of nuclear fumarase at Thr 236, which leads to an interaction between fumarase and the histone variant H2A.Z at DNA double-strand break (DSB) regions.
Viral and Cellular Genomes Activate Distinct DNA Damage Responses.
O'Shea et al., Los Angeles, United States. In Cell, 27 Sep 2015
In contrast to chromosomal breaks, ATM activation is not amplified by H2AX across megabases of chromatin to induce global signaling and replicative arrest.
Reconstitution of mitotic chromatids with a minimum set of purified factors.
Hirano et al., Wako, Japan. In Nat Cell Biol, Aug 2015
We find that octameric nucleosomes containing the embryonic variant H2A.X-F are highly susceptible to FACT and function as the most productive substrate for subsequent actions of topo II and condensin I. Cdk1 phosphorylation of condensin I is the sole mitosis-specific modification required for chromatid reconstitution.
Chromatin Dynamics in Vivo: A Game of Musical Chairs.
Dalal et al., Bethesda, United States. In Genes (basel), Dec 2014
In this review, we will focus on the localization of the major histone variants H3.3, CENP-A, H2A.Z, and macroH2A, as well as how these variants have evolved, their structural differences, and their functional significance in vivo.
The Structural Determinants behind the Epigenetic Role of Histone Variants.
Ausió et al., Victoria, Canada. In Genes (basel), Dec 2014
A picture starts to emerge in which histone H2A variants, in addition to their individual specific contributions to the nucleosome structure and dynamics, globally impair the accessibility of linker histones to defined chromatin locations and may have important consequences for determining different states of chromatin metabolism.
Fine-tuning the ubiquitin code at DNA double-strand breaks: deubiquitinating enzymes at work.
Citterio, Amsterdam, Netherlands. In Front Genet, Dec 2014
Here, we discuss the current knowledge of how ub-mediated signaling at DSBs is controlled by DUBs, with main focus on DUBs targeting histone H2A and on their recent implication in stem cell biology and cancer.
RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling.
Sixma et al., Amsterdam, Netherlands. In Cell, 2012
Ubiquitin-dependent signaling during the DNA damage response (DDR) to double-strand breaks is initiated by two E3 ligases, RNF8 and RNF168, targeting histone H2A and H2AX. Study shows that ubiquitin chains per se are insufficient for signaling, but RNF168 target ubiquitination is required for DDR damage.
Redundant and nonredundant functions of ATM and H2AX in αβ T-lineage lymphocytes.
Bassing et al., Philadelphia, United States. In J Immunol, 2012
Both redundant and nonredundant functions of H2AX and ATM transgenes are required for normal recombination of T cell receptor (TCR) loci, proliferative expansion of developing thymocytes, and maintenance of genomic stability in cycling alphabeta T-lineage cells.
High basal γH2AX levels sustain self-renewal of mouse embryonic and induced pluripotent stem cells.
Giachino et al., Torino, Italy. In Stem Cells, 2012
Findings suggest a novel function of H2AX that expands the knowledge of this histone variant beyond its role in DNA damage and into a new specialized biological function in mouse pluripotent stem cells.
Deregulation of BRCA1 leads to impaired spatiotemporal dynamics of γ-H2AX and DNA damage responses in Huntington's disease.
Ryu et al., Seoul, South Korea. In Mol Neurobiol, 2012
BRCA1 directly modulates the spatiotemporal dynamics of gamma-H2AX upon genotoxic stress and serves as a molecular maker for neuronal DNA damage response in Huntington's disease.
Loss of CCDC6, the first identified RET partner gene, affects pH2AX S139 levels and accelerates mitotic entry upon DNA damage.
Celetti et al., Napoli, Italy. In Plos One, 2011
Following genotoxic stress, loss or inactivation of CCDC6 in cancers that carry the CCDC6 fusion, accelerates the dephosphorylation of pH2AX S139, resulting in defective G2 arrest and premature mitotic entry.
More papers using H2A antibodies
Complementary functions of ATM and H2AX in development and suppression of genomic instability
Malle Ernst et al., In DNA Repair, 2007
... , termed γ-H2AX, Cell Signaling Technology, Beverly, MA, USA); ...
Enhanced inhibition of adipogenesis and induction of apoptosis in 3T3-L1 adipocytes with combinations of resveratrol and quercetin
Cook John A. et al., In Frontiers in Oncology, 2007
... CA, USA); mouse monoclonal anti-phospho histone H2AX (Ser139), clone JBW301, and rabbit antiserum to histone H2A (acidic patch) were from Upstate Cell Signaling Solutions (Temecula, CA, USA) ...
Integrin-mediated transforming growth factor-beta activation regulates homeostasis of the pulmonary epithelial-mesenchymal trophic unit
Kuwano Kazuyoshi et al., In Oncoimmunology, 2005
... used were rabbit anti-LC3 (Novus), rabbit anti-ATG5 (Novus), mouse anti-p21 (Cell signaling Technology), rabbit anti-phospho-histone H2A.X (Ser139) (Cell signaling Technology), mouse anti-poly and ...
Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster.
Oshima Robert, In PLoS ONE, 2005
... Cruz Biotechnology), Ki67 (Lab Vision, Fremont, CA, USA); mouse monoclonal antibodies for phosphor-H2AX, Ser 139, (γH2AX) (BioLegend, San Diego, CA, USA), ...
The comet assay: a method to measure DNA damage in individual cells
Fritz G et al., In Cell Death & Disease, 2005
... H2AX anti-rabbit antibody (pS139) (Epitomics, Burlingame, CA, USA), ERK2 ...
Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair.
Kirkpatrick David T., In PLoS ONE, 2004
... anti-CHK2 (Santa Cruz), anti-phospho-CHK1 (Ser 345) (Cell signaling), anti-phospho-CHK2 (Thr 68) (Cell signaling), anti-γH2AX (Upstate), anti-H2AX (Abcam), anti-HA (HA-7, Sigma) ...
Histochemical localization of alkaline phosphatase activity in decalcified bone and cartilage
Tang Liping et al., In Scientific Reports, 2001
... Primary antibodies CD34, CD44, CD105, CD29, SCA-1, CD45, osteocalcin, 53BP1, γ-H2AX, were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA) ...
Functional interaction between BLM helicase and 53BP1 in a Chk1-mediated pathway during S-phase arrest
Harris Curtis C. et al., In The Journal of Cell Biology, 2001
... Anti-γ-H2AX: rabbit polyclonal PC-100 (Trevigen).
Crystal structure of a nucleosome core particle containing the variant histone H2A.Z
Chrivia John C. et al., In International Journal of Cell Biology, 1999
... Other commercial antibodies were histone H2A.Z polyclonal (Abcam, ab4174), histone H3 polyclonal ...
A peptidomimetic inhibitor of farnesyl:protein transferase blocks the anchorage-dependent and -independent growth of human tumor cell lines
Nie Daotai et al., In Cancer Cell International, 1994
... from Clontech; Lamin A/C (sc-20681) and emerin antibodies were purchased from Santa Cruz biotechnology; anti-phosphorylated histone-H2AX polyclonal antibody was from Trevigen.
Methods for clonal growth and serial cultivation of normal human epidermal keratinocytes and mesothelial cells.
Karl Mike O., In PLoS ONE, 1988
... (Abcam), anti Keratin 1,10,11 (Ks 8.60; Abcam), anti Keratin 10 (RKSE60; Abcam), anti-keratin 1 (AF87; Covance), anti-γH2AX (Ser139; Millipore), anti-BrdU (B44; BD Biosciences), anti-phospho-histone H3 (P-H3; Upstate ...
Changes in the cell kinetics of pig epidermis after single doses of X rays.
Borgmann Kerstin, In PLoS ONE, 1987
... Mouse anti-γ-H2AX, JBW3011/500Milipore ...
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