The developmental potential of iPSCs is greatly influenced by reprogramming factor selection.
Jerusalem, Israel. In Cell Stem Cell, 04 Oct 2014
Although differentially methylated regions, transcript number of master regulators, establishment of specific superenhancers, and global aneuploidy were comparable between high- and low-quality lines, aberrant gene expression, trisomy of chromosome 8, and abnormal H2A.X deposition were distinguishing features that could potentially also be applicable to human.
Quality control: H2A.X links to better iPSCs.
Shanghai, China. In Cell Stem Cell, 04 Oct 2014
Two papers in this issue of Cell Stem Cell (Buganim et al., 2014; Wu et al., 2014) now show that H2A.X deposition can serve as a functional epigenetic marker for distinguishing the quality of iPSCs, thus providing insights for better reprogramming methods.
[Influence of Calcineurin on Apoptosis of pre-B Lymphocytes and the Leukemia Cells Derived from pre-B lymphocytes].
Zhangjiakou, China. In Zhongguo Shi Yan Xue Ye Xue Za Zhi, 30 Sep 2014
The quantitative real-time PCR was used to detect the differential expressions of H2AX-associated phosphakinase ATM, ATR, DNA-PKs, JNK1, P38 and the γ-H2AX-related phosphatase PP1, PP2A, calcineurin, PP4, PP6, PP5 between S9 and S4C2 cell lines.
RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling.
More papers using
Amsterdam, Netherlands. In Cell, 2012
Ubiquitin-dependent signaling during the DNA damage response (DDR) to double-strand breaks is initiated by two E3 ligases, RNF8 and RNF168, targeting histone H2A and H2AX. Study shows that ubiquitin chains per se are insufficient for signaling, but RNF168 target ubiquitination is required for DDR damage.
Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster.
In PLoS ONE, 2005
... Cruz Biotechnology), Ki67 (Lab Vision, Fremont, CA, USA); mouse monoclonal antibodies for phosphor-H2AX, Ser 139, (γH2AX) (BioLegend, San Diego, CA, USA), ... Histochemical localization of alkaline phosphatase activity in decalcified bone and cartilage
In Scientific Reports, 2001
... Primary antibodies CD34, CD44, CD105, CD29, SCA-1, CD45, osteocalcin, 53BP1, γ-H2AX, were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA) ...