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Golgi reassembly stacking protein 1, 65kDa

GRASP65, p65
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: p65, V1a, Interleukin-6, CAN, p38
Papers using GRASP65 antibodies
Alkaloids from Chelidonium majus and their inhibitory effects on LPS-induced NO production in RAW264. 7 cells
Jee Seon Young et al., In Evidence-based Complementary and Alternative Medicine : eCAM, 2010
... B p65, and Lamin A antibodies were supplied from Santa Cruz Biotechnology (Santa Cruz, CA, USA) ...
Autophagy negatively regulates keratinocyte inflammatory responses via scaffolding protein p62/SQSTM1
Koya Daisuke et al., In Experimental Diabetes Research, 2010
... B (p65) antibodies were purchased from Abcam (Cambridge, MA) ...
YK-4-279 Inhibits ERG and ETV1 Mediated Prostate Cancer Cell Invasion.
Li Jun, In PLoS ONE, 2010
... Primary antibodies against MyD88, TRIF, IRAK4, NF-κB p65 and phospho-NF-κB (P-p65) were purchased from Cell Signaling Technology (Beverly, MA, USA) ...
Phlorofucofuroeckol A inhibits the LPS-stimulated iNOS and COX-2 expressions in macrophages via inhibition of NF-κB, Akt, and p38 MAPK
Lee Jai-Heon et al., In Evidence-based Complementary and Alternative Medicine : eCAM, 2010
... Antibodies specific for iNOS, COX-2, p50, p65, IκB-α, p-IκB-α, and goat anti-rabbit IgG-FITC were obtained from Santa Cruz Biotechnology (CA, USA), while antibodies ...
SIRT1 suppresses activator protein-1 transcriptional activity and cyclooxygenase-2 expression in macrophages.
Huang Chuanshu et al., In Environmental Health Perspectives, 2009
... from Cell Signaling Technology (Beverly, MA, USA); Antibodies against COX-2, Jun-B, Jun-D, c-Fos, Fra-1, and p65 from Santa Cruz Biotechnology (Santa Cruz, CA, USA); ...
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Papers on GRASP65
Chronic Normobaric Hypoxia Induces Pulmonary Hypertension in Rats: Role of NF-κB.
Gong et al., Wenzhou, China. In High Alt Med Biol, Feb 2016
Compared to the normoxia exposure group, rats exposed to chronic normobaric hypoxia showed an increased NF-κB activity, measured by increased nuclear translocation of p50 and p65 proteins, an increased inflammatory gene expression in the lungs, elevated mean pulmonary arterial blood pressure and mean right ventricular pressure, right ventricular hypertrophy, as assessed by right ventricle-to-left ventricle plus septum weight ratio, and right heart dysfunction.
Profilin-PTEN interaction suppresses NF-kappa B activation via inhibition of IKK phosphorylation.
Manna et al., Hyderābād, India. In Biochem J, Feb 2016
Profilin overexpressing cells show low basal activity of IKK, high amount of cytoplasmic IκBα and p65, and low nuclear NF-κB DNA binding activity.
Cellular functions of programmed cell death 5.
Chen et al., Beijing, China. In Biochim Biophys Acta, Feb 2016
The stability and expression of PDCD5 are finely regulated by other molecules, such as NF-κB p65, OTUD5, YAF2 and DNAJB1.
Loss-of-function mutations in TNFAIP3 leading to A20 haploinsufficiency cause an early-onset autoinflammatory disease.
Aksentijevich et al., Bethesda, United States. In Nat Genet, Jan 2016
Patient-derived cells show increased degradation of IκBα and nuclear translocation of the NF-κB p65 subunit together with increased expression of NF-κB-mediated proinflammatory cytokines.
Cyclin-Dependent Kinases as Coregulators of Inflammatory Gene Expression.
Kracht et al., Gießen, Germany. In Trends Pharmacol Sci, Jan 2016
The ability of CDK6 to trigger the expression of VEGF-A and p16(INK4A) and to recruit the NF-κB subunit p65 to its target sites is largely independent of its kinase function.
NFKB1: a Suppressor of Inflammation, Ageing and Cancer.
Wilson et al., Newcastle upon Tyne, United Kingdom. In Febs J, Jan 2016
Therefore, while the majority of research in this field has focussed on the upstream signalling pathways leading to NF-κB activation or the function of other NF-κB subunits, such as RelA (p65), these data demonstrate a critical role for NFKB1, potentially revealing new strategies for targeting this pathway in inflammatory diseases and cancer.
An Abnormal Nitric Oxide Metabolism Contributes to Brain Oxidative Stress in the Mouse Model for the Fragile X Syndrome, a Possible Role in Intellectual Disability.
de Diego-Otero et al., Málaga, Spain. In Oxid Med Cell Longev, Dec 2015
The aim was to investigate the nitric oxide metabolism, protein nitration level, the expression of NOS isoforms, and furthermore the activation of the nuclear factor NF-κB-p65 subunit in different brain areas on the fragile X mouse model.
Non-Neuronal Cells Are Required to Mediate the Effects of Neuroinflammation: Results from a Neuron-Enriched Culture System.
Herrup et al., Hong Kong, Hong Kong. In Plos One, Dec 2015
Compared with mixed culture, the protein levels of NFκB p65 and the gene expression of several cytokine receptors were altered.
Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions.
Feigon et al., Los Angeles, United States. In Science, Nov 2015
This study reveals the paths of TER in the TERT-TER-p65 catalytic core and single-stranded DNA exit; extensive subunit interactions of the TERT essential N-terminal domain, p50, and TEB; and other subunit identities and structures, including p19 and p45C crystal structures.
Orphan Nuclear Receptor ERRα Controls Macrophage Metabolic Signaling and A20 Expression to Negatively Regulate TLR-Induced Inflammation.
Jo et al., Taejŏn, South Korea. In Immunity, Aug 2015
Further, ERRα was required for the regulation of NF-κB signaling by controlling p65 acetylation via maintenance of NAD(+) levels and sirtuin 1 activation.
KPC1-mediated ubiquitination and proteasomal processing of NF-κB1 p105 to p50 restricts tumor growth.
Ciechanover et al., Haifa, Israel. In Cell, May 2015
Also, overabundance of p50 downregulates p65, suggesting that a p50-p50 homodimer may modulate transcription in place of the tumorigenic p50-p65.
Effect of UBE2L3 genotype on regulation of the linear ubiquitin chain assembly complex in systemic lupus erythematosus.
Vyse et al., London, United Kingdom. In Lancet, Mar 2015
p65 NF-κB translocation in ex-vivo B cells and monocytes from genotyped healthy individuals was quantified by imaging flow cytometry.
GRASPs in Golgi Structure and Function.
Wang et al., Ann Arbor, United States. In Front Cell Dev Biol, 2014
The Golgi ReAssembly Stacking Proteins, GRASP65, and GRASP55, are so far the only proteins shown to function in Golgi stacking.
Mechanisms of Photoaging and Cutaneous Photocarcinogenesis, and Photoprotective Strategies with Phytochemicals.
González et al., Málaga, Spain. In Antioxidants (basel), 2014
UV radiation reduces cellular antioxidant status by generating reactive oxygen species (ROS), and the resultant oxidative stress alters signal transduction pathways such as the mitogen-activated protein kinase (MAPK), the nuclear factor-kappa beta (NF-κB)/p65, the janus kinase (JAK), signal transduction and activation of transcription (STAT) and the nuclear factor erythroid 2-related factor 2 (Nrf2).
Oestradiol Treatment Counteracts the Effect of Fructose-Rich Diet on Matrix Metalloproteinase 9 Expression and NFκB Activation.
Stankovic et al., Belgrade, Serbia. In Folia Biol (praha), 2014
We further assessed the effect of fructose-rich diet and oestradiol on NFκB activation, measured as the level of p65 phosphorylation at Ser 276.
An OBSL1-Cul7Fbxw8 ubiquitin ligase signaling mechanism regulates Golgi morphology and dendrite patterning.
Bonni et al., Boston, United States. In Plos Biol, 2011
Dendrites in Grasp65 knockdown granule neurons are longer and more highly branched than dendrites in control neurons.
Mitotic inhibition of GRASP65 organelle tethering involves Polo-like kinase 1 (PLK1) phosphorylation proximate to an internal PDZ ligand.
Linstedt et al., Pittsburgh, United States. In J Biol Chem, 2011
the mechanism of phosphoinhibition as direct inhibition by PLK1 of the PDZ ligand underlying the GRASP65 self-interaction.
The role of GRASP65 in Golgi cisternal stacking and cell cycle progression.
Wang et al., Ann Arbor, United States. In Traffic, 2010
GRASP65 has a role in Golgi cisternal stacking and cell cycle progression
GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking.
Wang et al., Ann Arbor, United States. In J Cell Biol, 2010
These results demonstrate that GRASP55 and GRASP65 stack mammalian Golgi cisternae via a common mechanism.
Caspase cleavage of the Golgi stacking factor GRASP65 is required for Fas/CD95-mediated apoptosis.
Lane et al., Bristol, United Kingdom. In Cell Death Dis, 2009
The C-terminal fragments of GRASP65 produced following caspase cleavage are targeted to mitochondria, and ectopic expression of these sensitises HeLa cells to Fas ligand.
More papers using GRASP65 antibodies
Srt1720, srt2183, srt1460, and resveratrol are not direct activators of sirt1
Matter Christian M. et al., In Aging (Albany NY), 2009
... Immunoprecipitated samples were immunoblotted with rabbit anti-acK310-p65 (Abcam), and the total lysates ...
Calorie restriction enhances cell adaptation to hypoxia through Sirt1-dependent mitochondrial autophagy in mouse aged kidney.
Chatziantoniou Christos, In PLoS ONE, 2009
... phospho-IκBα, p38-MAPK, phospho-p38-MAPK (Thr180/Tyr182), p44/42 MAPK, phospho-p44/42 MAPK, SAPK/JNK, phospho-SAPK/JNK (T183/Y185), Histone H3 and NFκB p65 were from Cell Signaling Technology (Beverly, MA, USA) ...
EP2 receptor activation by prostaglandin E2 leads to induction of HO-1 via PKA and PI3K pathways in C6 cells
Yang Chuen-Mao et al., In Journal of Neuroinflammation, 2008
... Anti-phospho-NF-κB p65 antibody was purchased from Cell Signaling (Danver, MA, USA) ...
Combination chemotherapy of nafamostat mesilate with gemcitabine for pancreatic cancer targeting NF-kappaB activation.
Chandra Dhyan, In PLoS ONE, 2008
... Antibodies against Bcl-2, Bax and p-IκB-α (Ser32/36) (rabbit polyclonal), Bcl-xl and NF-κB/p65 (rabbit monoclonal), and IκB-α (mouse monoclonal) were obtained from Cell Signaling Technology (Beverly, MA) ...
Constitutively activated ALK2 and increased SMAD1/5 cooperatively induce bone morphogenetic protein signaling in fibrodysplasia ossificans progressiva
Huang Yu, In PLoS ONE, 2008
... Phospho-Smad1/5/8, Smad1, phospho-NF-κB p65 and NF-κB p65 antibodies were purchased from Cell Signaling Technology (Beverly, MA) ...
Effects of the sesquiterpene lactone parthenolide on prostate tumor-initiating cells: An integrated molecular profiling approach.
Sarkar Fazlul H., In PLoS ONE, 2008
... -phospho-EGFR antibody (1173) recognizing the EGFR form phosphorylated at tyrosine 1173 and rabbit polyclonal anti-NF-κB p65 protein subunit (C-20) were purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA) ...
Rosmanol potently inhibits lipopolysaccharide-induced iNOS and COX-2 expression through downregulating MAPK, NF-κB, STAT3 and C/EBP signaling pathways
Yang Chia-Ron et al., In Journal of Biomedical Science, 2008
... Mouse monoclonal anti-NF-κB p65 antibody was obtained from BioVision (Mountain View, CA, USA) ...
IL-8 as antibody therapeutic target in inflammatory diseases: reduction of clinical activity in palmoplantar pustulosis.
Chodosh James et al., In Molecular Vision, 2007
... NFκB p65-specific and jumbled control siRNA were purchased from Imgenex (San Diego, CA) ...
miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells.
Afford Simon, In PLoS ONE, 2007
... -p65, anti-rabbit IgG-horseradish peroxidase (HRP) conjugates, and rabbit anti-mouse IgG-HRP conjugates antibodies were purchased from Cell Signaling (Beverly, MA, USA) ...
Compensatory IKKα activation of classical NF-κB signaling during IKKβ inhibition identified by an RNA interference screen.
Herrera-Estrella Alfredo, In PLoS ONE, 2007
... Antibodies to phospho-specific NF-κB p65 (Ser-536) and IκBα (Ser 32/36) were obtained from Cell Signaling.
Inhibitory effect of triptolide on interleukin-18 and its receptor in rheumatoid arthritis synovial fibroblasts.
Nishida Teruo et al., In Molecular Vision, 2007
... B-alpha (IκB-α) were obtained from Cell Signaling (Beverly, MA), and rabbit polyclonal antibodies to the p65 subunit of Nuclear Factor-kappa B (NF-κB) were from Santa Cruz Biotechnology (Santa Cruz, CA) ...
Development of reverse genetics systems for bluetongue virus: recovery of infectious virus from synthetic RNA transcripts
Roy Polly et al., In Virology Journal, 2007
... Anti-IkBα (E:130), anti-IkBβ (ab7547), anti-NF-κB (p50/p105) and anti-NF-κB (p65) polyclonal antibodies were purchased from Abcam.
Decoy receptor 3 expression in AsPC-1 human pancreatic adenocarcinoma cells via the phosphatidylinositol 3-kinase-, Akt-, and NF-κB-dependent pathway.
Bandeira de Melo Christianne, In PLoS ONE, 2007
... Mouse monoclonal anti-NF-κB p65 antibody was obtained from BioVision (Mountain View, CA, USA) ...
Chlamydia pneumoniae directly interferes with HIF-1alpha stabilization in human host cells.
Roy Craig R., In PLoS Pathogens, 2006
... anti-keratin-18, mouse anti-α-tubulin, and mouse anti-vimentin (all from Sigma-Aldrich); rabbit anti-cyclin B1, mouse anti-nectin-1, mouse anti-p65/RelA and bovine anti-sheep-HRP (all from Santa Cruz Biotechnology); rabbit anti-Bik, rabbit anti-vimentin, ...
Ultrasound induces cyclooxygenase-2 expression through integrin, integrin-linked kinase, Akt, NF-kappaB and p300 pathway in human chondrocytes
Tang Chih-Hsin et al., In Molecular Cancer, 2006
... Rabbit polyclonal antibody specific for p-IKKα/β, p-PLCβ3, p-PKCα, p-c-Src and p-p65 were purchased from Cell Signaling and Neuroscience (Danvers, MA) ...
TWEAK-FN14 signaling induces lysosomal degradation of a cIAP1–TRAF2 complex to sensitize tumor cells to TNFα
Silke John et al., In The Journal of Cell Biology, 2006
... anti-TRAF2 (Santa Cruz Biotechnology, Inc.), anti-FN14 (Cell Signaling Technology), anti-NIK (Cell Signaling Technology), anti–phospho–NF-κB (Ser536) p65 (Cell Signaling Technology), anti-IκB, (Cell Signaling ...
Non-enzymatic glycation of bone collagen modifies osteoclastic activity and differention
Wolf Gunter et al., In Arthritis Research & Therapy, 2006
... electrophoretic mobility shift assay (EMSA) the following were used: NFκB consensus and mutant oligonucleotides, anti-NFκB p65(A)X (Santa Cruz Biotech, Santa Cruz, CA, USA), T4 Polynucleotide Kinase and Reaction Buffer (New England Biolabs Inc., Ipswich, MA, USA), ...
Curcumin inhibits tumor growth and angiogenesis in ovarian carcinoma by targeting the nuclear factor-κB pathway
Li Hao et al., In Evidence-based Complementary and Alternative Medicine : eCAM, 2006
... B (P65) antibody and mouse anti-human IκBα antibody (Cell Signaling Co., Ltd., USA), and ...
Glycogen synthase kinase-3 (GSK3): inflammation, diseases, and therapeutics
Kuo Jon-Son et al., In Journal of Neuroinflammation, 2006
... Polyclonal rabbit anti-acetyl p65 and polyclonal rabbit anti-phospho-p65 (Ser276) were obtained from Abcam (Cambridge, UK) ...
The Role of the NADPH Oxidase Complex, p38 MAPK, and Akt in Regulating Human Monocyte/Macrophage Survival.
Cheriyath Venugopalan, In PLoS ONE, 2006
... Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA) ...
Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in active Crohn's disease
Han Xiaonan et al., In EMBO Molecular Medicine, 2006
... specific for MLC and phosphorylated MLC (serine 19) (pMLC), phospho-tyrosin694 specific STAT5 (pSTAT5), phospho-serine276 p65 (P-p65) antibodies were from Cell Signaling Technology (Danvers, MA) ...
A role for the mitogen-activated protein kinase kinase kinase 1 in epithelial wound healing.
Connon Che John, In PLoS ONE, 2005
... purchased from Cell Signaling Technology (Danvers, MA); Antibodies for total JNK, total MKK6 and total p65 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA); Antibodies ...
Activated AKT regulates NF-κB activation, p53 inhibition and cell survival in HTLV-1-transformed cells
Simmet Thomas et al., In Journal of Biomedicine and Biotechnology, 2004
... , phospho-ERK1/2, phospho-p38, phospho-Akt (Ser473), phospho-p65 Ser536, and phospho-p65 Ser276-Cell Signaling Technology (Danvers, MA); CCL20-R&D ...
Divergent responses of chondrocytes and endothelial cells to shear stress: cross-talk among COX-2, the phase 2 response, and apoptosis.
Zheng Song Guo, In PLoS ONE, 2004
... p-Akt (Ser473), CREB, p-CREB (Ser133), ERK1/2, p-ERK1/2 (Thr 202/Tyr 204), NF-κB p65, p-p65 (Ser276) and p-p65 (Ser536) were purchased from Cell Signaling Technology, Inc ...
Cells migrating to sites of tissue damage in response to the danger signal HMGB1 require NF-κB activation
Bianchi Marco E. et al., In The Journal of Cell Biology, 2004
... Rabbit polyclonal antibodies against p65/RelA were obtained from Calbiochem and Santa Cruz Biotechnology, Inc ...
Association of NOD1 polymorphisms with atopic eczema and related phenotypes.
Bereswill Stefan, In PLoS ONE, 2004
... Antibodies against NF-κB p65, IκBα and IKKα were obtained from Santa Cruz Biotechnology (Santa Cruz, USA) whereas ...
Nuclear factor-kappaB inhibitors as sensitizers to anticancer drugs
Bauer Joseph Alan, In PLoS ONE, 2004
... The antibodies mouse monoclonal anti-P-gp, rabbit polyclonal anti-NF-κB p65 were purchased from Cell Signaling (MA), mouse monoclonal anti-β-actin ...
Overexpression of HSP-72 confers cytoprotection in experimental peritoneal dialysis
Neuhofer Wolfgang et al., In Mediators of Inflammation, 2003
... from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-actin antibody was from Sigma; anti-p65 and anti-phospho-p65 (Ser536) and horseradish peroxidase-conjugated anti-rabbit IgG were purchased from Cell Signaling (Beverly, MA, USA); anti-histone ...
Effects of the EGFR/HER2 kinase inhibitor GW572016 on EGFR- and HER2-overexpressing breast cancer cell line proliferation, radiosensitization, and resistance
Baldwin Albert S et al., In Oncogene, 2003
... antibodies against Her2, IKKα clone 14A231 and IKKβ clone10AG2 and p100/p52 from Millipore, antibodies against p65 and p50 (supershift), β-tubulin and IKKγ from Santa Cruz Biotechnology, antibody against total p65 ...
Pioglitazone treatment activates AMP-activated protein kinase in rat liver and adipose tissue in vivo
Xu Haiyan, In PLoS ONE, 2003
... Phospho-AMPK (Thr172) and acetyl-p65 (lysine-310) were purchased from Cell Signaling (Beverly, MA) ...
Inhibition of interleukin-12 p40 transcription and NF-kappaB activation by nitric oxide in murine macrophages and dendritic cells
Plevin Robin et al., In Molecular Immunology, 2003
... MAP kinase and NF-κB (p65 isoform) antibodies were from Santa Cruz Biotechnology (CA, USA) and rabbit ...
Signaling pathways responsible for cancer cell invasion as targets for cancer therapy.
Tsilibary Effie C., In PLoS ONE, 2003
... and anti-rabbit IgG-conjugated horseradish peroxidase, rabbit polyclonal antibodies specific for p-MEK, MEK, p-ERK, ERK, p-p65, p65, CCR5 siRNA, control siRNA, control shRNA plasmid, and CCL5 shRNA plasmid were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) ...
Nuclear factor-(kappa)B modulates the p53 response in neurons exposed to DNA damage
Caccamo Daniela et al., In BMC Neuroscience, 2003
... polyclonal antibody against p53, mouse monoclonal antibodies against β-actin as well as p50 (sc-8414X) and p65 (sc-8008X) NF-κB subunits, and the NF-κB inhibitor, SN50, were from Santa Cruz Biotechnology (DBA, Milano, Italy) ...
Protective role of metallothionein in acute lung injury induced by bacterial endotoxin
Matsura Tatsuya et al., In Journal of Clinical Biochemistry and Nutrition, 2003
... Rabbit anti-NF-κB p65 (H-286) polyclonal antibody was purchased from Santa Cruz Biotechnology, Inc ...
Cisplatin induces endoplasmic reticulum stress and nucleus-independent apoptotic signaling
Wong Nai Sum, In PLoS ONE, 2002
... Anti-IκBα, anti-Bax, anti-MDM2, anti-p65, and anti-NF-κB p50 antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA) ...
Mechanism of steroid action and resistance in inflammation: Corticoisteroids, eosinophils and bronchial epithelial cells: new insights into the resolution of inflammation in asthma
Zhang Xianzhi et al., In Journal of Inflammation (London, England), 2002
... (Alexis, Lausen, Switzerland), CD95 monoclonal antibody (clone CH-11; Immunotech, Marseille, France), NF-kB p65 and acetyl-NF-kB p65 (Lys310) antibodies (Cell Signaling Technology, Inc., Danvers, USA), ...
Intracellular Recognition of Lipopolysaccharide by Toll-like Receptor 4 in Intestinal Epithelial Cells
Normark Staffan et al., In The Journal of Experimental Medicine, 2002
... the rabbit anti–MyD88 antibody, the mouse anti–IL-1R–associated kinase (IRAK)-1 antibody, as well as the rabbit anti–p65 antibody were purchased from Santa Cruz Biotechnology, Inc ...
Degradation of Promoter-bound p65/RelA Is Essential for the Prompt Termination of the Nuclear Factor κB Response
Natoli Gioacchino et al., In The Journal of Experimental Medicine, 2002
... Anti-p65 (C20) was from Santa Cruz Biotechnology, Inc., anti-IκBα monoclonal was ...
The phosphorylation status of nuclear NF-kappa B determines its association with CBP/p300 or HDAC-1
Bromberg Philip A. et al., In Environmental Health Perspectives, 2001
... We obtained anti-human p65 polyclonal antibody from Cayman Chemical (Ann Arbor, MI); ...
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method
Duffield Jeremy Stuart, In PLoS ONE, 2000
... Antibodies against phosphorylated or total MEK1/2 (Ser217/221), p38 MAPK (Thr180/Tyr182), and pNFkB/p65 (Ser536) were purchased from Cell signaling Technology ...
Rapid production of retroviruses for efficient gene delivery to mammalian cells using 293T cell-based systems.
Damania Blossom, In PLoS Pathogens, 2000
... (AC-74, Sigma), RIP1 (38, BD Transduction Laboratories), HA (16B12, Covance Inc.), HA (3F10, Roche), NF-κB p65 (F-6, Santa Cruz, or C22B4, Cell Signaling), LC3B (D11, Cell signaling), ...
Constitutive activation of nuclear factor-kappaB prevents TRAIL-induced apoptosis in renal cancer cells.
Ahmad Aamir, In PLoS ONE, 2000
... the following sources: anti-mouse and anti-rabbit IgGs, Bcl-2, Bcl-xL, Bax, TRAIL, DR4, DR5, PPAR, NF-κB p65 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA); p21, ...
Intimate adhesion of Neisseria meningitidis to human epithelial cells is under the control of the crgA gene, a novel LysR-type transcriptional regulator.
Roy Craig R., In PLoS Pathogens, 1999
... Mouse anti-AU1, anti-Myc, anti-FLAG, anti-HA, anti-hTNFR1 mAbs and anti-p65 and anti-p50 rabbit polyclonal antibodies were purchased from abcam (France) ...
Selective inhibition of NF-kappaB activation by a peptide that blocks the interaction of NEMO with the IkappaB kinase complex.
Toland Amanda Ewart, In PLoS ONE, 1999
... Antibodies for p50 (ab7971), p65 (ab32536 and ab7970) and MMP3 (ab53015) were from Abcam.
Role of MAP kinase cascades in inducing arginine transporters and nitric oxide synthetase in RAW 264.7 macrophages
Ma Jin Yeul et al., In BMC Complementary and Alternative Medicine, 1998
... β-actin, p38, phospho-p38, ERK, phospho-ERK, JNK, phospho-JNK and p65 monoclonal antibodies were purchased from cell signaling technology, Inc ...
Activation of nuclear factor-κB is necessary for myotrophin-induced cardiac hypertrophy
Sen Subha et al., In The Journal of Cell Biology, 1997
... The p65 antibody, GST–IκBα, and double-stranded NF-κB DNA (5′-AGT TGA GGG GAC TTT CCC AGG C-3′) were purchased from Santa Cruz Biotechnology, Inc ...
Preventive effects of the plant isoflavones, daidzin and genistin, on bone loss in ovariectomized rats fed a calcium-deficient diet
Ma Jin Yeul et al., In Evidence-based Complementary and Alternative Medicine : eCAM, 1997
... , phospho-p65 (Ser536), and p65 were purchased from Cell Signaling Technology (Danvers, MA, USA) ...
Clathrin gene expression is androgen regulated in the prostate.
Keen James, In PLoS ONE, 1997
... Antibodies against NF-κB p65, IκBα, CLCa and IKKα were obtained from Santa Cruz Biotechnology (Santa Cruz, USA) while ...
p38 and extracellular signal-regulated kinase mitogen-activated protein kinase pathways are required for nuclear factor-kappaB p65 transactivation mediated by tumor necrosis factor
Feng Gen-Sheng et al., In The Journal of Experimental Medicine, 1997
... Polyclonal anti–Shp-2, antiphosphotyrosine (pY99), anti-TNFR1, anti–IL-1RI, p50, or p65 of NF-κB antibodies were from Santa Cruz Biotechnology, Inc ...
IKK/NF-κB regulates skeletal myogenesis via a signaling switch to inhibit differentiation and promote mitochondrial biogenesis
Guttridge Denis C. et al., In The Journal of Cell Biology, 1997
... Antibodies to p100–p52, IκBα (C21), IKKβ, IKKγ (FL419), myogenin (M-225), p38, MyoD (M-318), and p65 (N terminal) were obtained from Santa Cruz Biotechnology, Inc ...
Tumor necrosis factor-alpha in sera of obese patients: fall with weight loss
Goalstone Marc L et al., In Journal of Inflammation (London, England), 1997
... Primary antibodies to NFκB p65 (Cat# 4764) were from Cell Signaling (Boston, MA) ...
Cationic polystyrene NPs: preparation and characterization of a model drug carrier system for antisense oligonucleotides
Sun Wangqiang et al., In International Journal of Molecular Sciences, 1996
... Anti-p65 rabbit polyclonal antibody was obtained from Santa Cruz Biotechnology.
A role for 12-lipoxygenase in nerve cell death caused by glutathione depletion
Strosznajder Robert P. et al., In Neurochemical Research, 1996
... MP Biomedicals, Irvine CA, USA; anti-cytochrome c was purchased from BD Pharmingen, SD, USA anti-AIF and anti-p65 IgG were purchased from Santa Cruz Biotechnology, Santa Cruz CA, USA; ...
Transcription factor AP-1 regulation by mitogen-activated protein kinase signal transduction pathways
Tanaka Sakae et al., In The Journal of Cell Biology, 1995
... anti–v-src antibody (clone 327) from Oncogene Research Products, anti–Flag antibody from Sigma Chemicals, and anti–NF-κB (p65) antibody from Santa Cruz Biotechnology.
KLF2 Is a Novel Transcriptional Regulator of Endothelial Proinflammatory Activation
Jain Mukesh K. et al., In The Journal of Experimental Medicine, 1995
... Antibodies recognizing VCAM-1, intercellular cell adhesion molecule (ICAM)-1, p50, p65, IκB-α, phospho–IκB-α, and α-HA were purchased from Santa Cruz Biotechnology, Inc., p300 antibody (Labvision), ...
Bioconjugate Techniques.
Rao Jianghong, In PLoS ONE, 1995
... were blocked with 5% BSA in PBS and then incubated with anti-NF-κB (Rabbit polyclonal NF-κB p65(C-20), Santa Cruz Biotechnology, ®Inc.,Santa
The alpha 5 beta 1 integrin supports survival of cells on fibronectin and up-regulates Bcl-2 expression
Giachelli Cecilia M. et al., In The Journal of Cell Biology, 1994
... Rabbit polyclonal antibodies against NF-κB p65, p50 subunits were obtained from Santa Cruz Biotechnology ...
Quantitative measurement of sphingosine 1-phosphate in biological samples by acylation with radioactive acetic anhydride.
Neyrolles Olivier, In PLoS ONE, 1994
... Rabbit anti-mouse pp65 (RelA) antibody was purchased from Cell signaling.
Chondrogenesis in periosteal explants. An organ culture model for in vitro study.
Beier Frank, In PLoS ONE, 1993
... and transfection with siRNAs (100 nM for p65 and 50 nM for Sox9) was performed using ICAfectin 442 (Eurogentec) according to manufacturers' protocol ...
Rapid activation of the STAT3 transcription factor by granulocyte colony-stimulating factor
Nerurkar Pratibha V., In PLoS ONE, 1993
... Rabbit polyclonal antibody against p65 was purchased from BioVision, Inc ...
Mechanism of action of hydroxyurea
Yang Chuen-Mao et al., In Journal of Neuroinflammation, 1991
... )-p65 antibody kits were from Cell Signaling (Danver, MA, USA) ...
Tobacco smoke suppresses T cells but not antigen-presenting cells in the lung-associated lymph nodes.
Neyrolles Olivier, In PLoS ONE, 1989
... Rabbit polyclonal antibody against IκB-α and p65 were obtained from Santa Cruz Biotechnology (Heerhugowaard, The Netherlands) ...
Specific chromosome loss associated with the expression of tumorigenicity in human cell hybrids
Kitagawa T et al., In Oncogenesis, 1980
... The rabbit monoclonal antibody to human p65 was purchased from Epitomics (Burlingame, CA, USA) ...
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