A Death Notice for In-Vitro-Generated GM-CSF Dendritic Cells?
Gent, Belgium. In Immunity, 16 Jul 2015
Mouse bone marrow cells cultured with GM-CSF are often used to generate dendritic cells (DCs); in this issue of Immunity, Helft et al. (2015) show that this classical method produces heterogeneous populations of myeloid cells that are only distantly related to macrophages and DCs found in vivo.
Genome-wide transcription profiling in neutrophils in acute respiratory distress syndrome.
Cambridge, United Kingdom. In Lancet, Mar 2015
METHODS: GeneChip Affymetrix oligonucleotide arrays were used to assess global transcriptional profiles in highly pure PMNs from ventilated patients fulfilling the Berlin ARDS definition (n=10), in freshly isolated PMNs from age-matched and sex-matched healthy volunteers (n=10), and in healthy volunteer PMNs exposed in vitro to recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF) (1 ng/mL for 6 h).