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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Aug 2016.

Glutamate-cysteine ligase, modifier subunit

GCLM, GLCLR
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: glutamate-cysteine ligase, catalytic subunit, Nrf2, V1a, ACID, CAN
Papers using GCLM antibodies
Early onset senescence occurs when fibroblasts lack the glutamate-cyteine ligase modifier subunit
Supplier
Costa Lucio G. et al., In Journal of Toxicology, 2008
... Gclm-Hz mice, in the expected Mendelian ratios [21].To genotype pups, genomic DNA was isolated from ear punch tissue using a Qiagen DNeasy kit, and mice ...
Papers on GCLM
Simvastatin inhibits oxidative stress via the activation of nuclear factor erythroid 2-related factor 2 signaling in trophoblast cells.
New
Konishi et al., Kyoto, Japan. In J Obstet Gynaecol Res, Jan 2016
AIM: Nuclear factor erythroid 2-related factor 2 (Nrf2) is a key transcriptional regulator against oxidative stress through the induction of antioxidant and cytoprotective genes, such as heme oxygenase 1 (HO-1), glutamyl cysteine ligase catalytic (GCLC), and glutamyl cysteine ligase modulatory (GCLM).
Dioscin alleviates BDL- and DMN-induced hepatic fibrosis via Sirt1/Nrf2-mediated inhibition of p38 MAPK pathway.
New
Peng et al., Dalian, China. In Toxicol Appl Pharmacol, Jan 2016
Furthermore, dioscin markedly up-regulated the levels of sirtuin 1 (Sirt1), HO-1, GST, GCLC and GCLM via increasing the nuclear translocation of nuclear erythroid factor 2-related factor 2 (Nrf2), which in turn inhibited mitogen-activated protein kinase 14 (p38 MAPK) phosphorylation and reduced the levels of COL1A1, COL3A1, α-SMA and fibronectin.
Bach1 differentially regulates distinct Nrf2-dependent genes in human venous and coronary artery endothelial cells adapted to physiological oxygen levels.
New
Mann et al., London, United Kingdom. In Free Radic Biol Med, Jan 2016
Induction of GSH-related genes xCT and GCLM were oxygen and Bach1-insensitive during long-term culture under 5% O2, providing the first evidence that genes related to GSH synthesis mediate protection afforded by Nrf2-Keap1 defense pathway in cells adapted to physiological O2 levels encountered in vivo.
Insulin-like growth factor 1 specifically up-regulates expression of modifier subunit of glutamate-cysteine ligase and enhances glutathione synthesis in SH-SY5Y cells.
New
Katsuki et al., Kumamoto, Japan. In Eur J Pharmacol, Jan 2016
The first step of glutathione synthesis is catalyzed by glutamate-cysteine ligase (GCL), which is composed of catalytic and modifier subunits (GCLC and GCLM, respectively).
HNF1β drives glutathione (GSH) synthesis underlying intrinsic carboplatin resistance of ovarian clear cell carcinoma (OCCC).
New
Serpa et al., Lisbon, Portugal. In Tumour Biol, Dec 2015
Metabolic profiles, GSH quantification, HNF1β, and γ-glutamylcysteine ligase catalytic subunit (GCLC) and modifier subunit (GCLM) expression, cell cycle, and death were assessed in ES2 cell line (OCCC) and OVCAR3 cell line (OSC); HNF1β knockdown was performed in ES2 and murine model of subcutaneous and peritoneal OCCC tumors was established to test buthionine sulphoxamine (BSO), as a sensitizer to carboplatin.
Glutathione and thioredoxin antioxidant pathways synergize to drive cancer initiation and progression.
New
Impact
Mak et al., Toronto, Canada. In Cancer Cell, Mar 2015
Here, we demonstrate that synthesis of the antioxidant glutathione (GSH), driven by GCLM, is required for cancer initiation.
The Activation of Nrf2 and Its Downstream Regulated Genes Mediates the Antioxidative Activities of Xueshuan Xinmaining Tablet in Human Umbilical Vein Endothelial Cells.
Wang et al., Changchun, China. In Evid Based Complement Alternat Med, 2014
XXT enhanced the expressions of Keap1, Nrf2, and Nrf2-mediated genes, such as glutamate-cysteine ligase modifier subunit (GCLM), NAD(P)H: quinine oxidoreductase 1 (NQO1), heme oxygenase 1 (HMOX1), and glutathione peroxidase (GPX) in HUVECs.
Cytoprotective Role of Nrf2 in Electrical Pulse Stimulated C2C12 Myotube.
Shoda et al., Tsukuba, Japan. In Plos One, 2014
We also showed that expression of NQO1, HO-1 and GCLM increased after EPS-induced muscle contraction and was remarkably suppressed in cells with Nrf2 knockdown.
Ginseng alleviates cyclophosphamide-induced hepatotoxicity via reversing disordered homeostasis of glutathione and bile acid.
Li et al., Nanjing, China. In Sci Rep, 2014
Furthermore, ginseng could induce expression of GCLC, GCLM, GS and GST, which associate with the disposition of GSH, and expression of FXR, CYP7A1, NTCP and MRP 3, which play important roles in the synthesis and transport of bile acids.
Lico A Enhances Nrf2-Mediated Defense Mechanisms against t-BHP-Induced Oxidative Stress and Cell Death via Akt and ERK Activation in RAW 264.7 Cells.
Ci et al., Changchun, China. In Oxid Med Cell Longev, 2014
Our results indicated that Lico A significantly inhibited t-BHP-induced cytotoxicity, apoptosis, and reactive oxygen species (ROS) generation and reduced glutathione (GSH) depletion but increased the glutamate-cysteine ligase modifier (GCLM) subunit and the glutamate-cysteine ligase catalytic (GCLC) subunit genes expression.
Glutathione synthesis.
Review
Lu, Los Angeles, United States. In Biochim Biophys Acta, 2013
Key determinants of GSH synthesis are the availability of the sulfur amino acid precursor, cysteine, and the activity of the rate-limiting enzyme, glutamate cysteine ligase (GCL), which is composed of a catalytic (GCLC) and a modifier (GCLM) subunit.
Increased sensitivity to testicular toxicity of transplacental benzo[a]pyrene exposure in male glutamate cysteine ligase modifier subunit knockout (Gclm-/-) mice.
GeneRIF
Luderer et al., Irvine, United States. In Toxicol Sci, 2012
Data suggest that Gclm is not involved in spermatogenesis but is involved in shielding testis/epididymis from oxidative stress; Gclm knockout mice are fertile but more sensitive to transplacental testicular/epididymal toxicity of benzo[a]pyrene.
Behavioral phenotyping of glutathione-deficient mice: relevance to schizophrenia and bipolar disorder.
GeneRIF
Do et al., Lausanne, Switzerland. In Behav Brain Res, 2012
gclm KO mice showed a potentiated hyperlocomotor response to amphetamine, impaired sensorimotor gating, and altered social behavior compared to WT. These anomalies resemble important aspects of both SZ and the manic component of BP.
Lipid metabolism and body composition in Gclm(-/-) mice.
GeneRIF
Shertzer et al., Cincinnati, United States. In Toxicol Appl Pharmacol, 2012
Gclm is involved in the regulation of basal metabolic rate and metabolism of dietary lipid
Heterozygosity in the glutathione synthesis gene Gclm increases sensitivity to diesel exhaust particulate induced lung inflammation in mice.
GeneRIF
Kavanagh et al., Seattle, United States. In Inhal Toxicol, 2011
This finding indicates that GSH synthesis can mediate DEP-induced lung inflammation and suggests that polymorphisms in Gclm may be an important factor in determining adverse health outcomes in humans following inhalation of particulate matter.
Lack of maternal glutamate cysteine ligase modifier subunit (Gclm) decreases oocyte glutathione concentrations and disrupts preimplantation development in mice.
GeneRIF
Luderer et al., Irvine, United States. In Endocrinology, 2011
investigation of roles for maternal Gclm in preimplantation development: Gclm -/- mice (compared to Gclm +/+ littermates) exhibit lower litter size, fewer uterine implantation sites, delayed blastocyst development after in vitro fertilization
Structure, function, and post-translational regulation of the catalytic and modifier subunits of glutamate cysteine ligase.
Review
Kavanagh et al., Denver, United States. In Mol Aspects Med, 2009
GCL is a heterodimeric protein composed of catalytic (GCLC) and modifier (GCLM) subunits that are expressed from different genes.
Regulation of glutathione synthesis.
Review
Lu, Los Angeles, United States. In Mol Aspects Med, 2009
GCL is composed for a catalytic (GCLC) and modifier (GCLM) subunit and they are regulated at multiple levels and at times differentially.
Modulating GSH synthesis using glutamate cysteine ligase transgenic and gene-targeted mice.
Review
Kavanagh et al., Seattle, United States. In Drug Metab Rev, 2007
Gclm null mice exhibit low GSH levels and enhanced sensitivity to acetaminophen.
Cytochrome P450 2E1-dependent oxidant stress and upregulation of anti-oxidant defense in liver cells.
Review
Cederbaum, New York City, United States. In J Gastroenterol Hepatol, 2006
The E47 cells had higher glutathione levels than control HepG2 cells due to activation of the genes encoding the heavy and light subunits of gamma glutamyl cysteine synthetase (GCLC and GCLM).
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