Purification and characterization of a novel polysaccharide-peptide complex from Clinacanthus nutans Lindau leaves.
Zhenjiang, China. In Carbohydr Polym, Mar 2016
Methylation analysis, FT-IR, and (1)H NMR spectroscopy analysis revealed that CNP-1-2 might have a backbone consisting of 1,4-linked Glcp, 1,3-linked Glcp, 1,3-linked Manp, 1,4-linked Galp, 1,2,6-linked Galp and 1,2,6-linked Galp.
Site-specific integration and constitutive expression of key genes into Escherichia coli chromosome increases shikimic acid yields.
Shanghai, China. In Enzyme Microb Technol, Jan 2016
Herein, crucial genes aroG, aroB, tktA, aroE (encoding 3-deoxy-d-arabinoheptulosonate-7-phosphate synthase, dehydroquinate synthase, transketolase and shikimate dehydrogenase, respectively) of SA pathway and glk, galP (encoding glucokinase and galactose permease) were integrated into the locus of ptsHIcrr (phosphoenolpyruvate: carbohydrate phosphotransferase system operon) in a shikimate kinase genetic defect strain Escherichia coli BW25113 (ΔaroL/aroK, DE3).
Mechanism-based candidate inhibitors of uridine diphosphate galactopyranose mutase (UGM).
Saskatoon, Canada. In Carbohydr Res, Jan 2016
Uridine diphosphate-galactopyranose mutase (UGM), an enzyme found in many eukaryotic and prokaryotic human pathogens, catalyzes the interconversion of UDP-galactopyranose (UDP-Galp) and UDP-galactofuranose (UDP-Galf), the latter being used as the biosynthetic precursor of the galactofuranose polymer portion of the mycobacterium cell wall.
Antitumor and immunomodulatory activity of a water-soluble polysaccharide from Grifola frondosa.
Zhenjiang, China. In Carbohydr Polym, Jan 2016
The results revealed that GP11 was composed of → 1)-D-Manp-(6 →,→ 1)-D-Glcp-(4 →,→ 1)-D-Galp-(6 → and → 2,3,6)-D-Glcp-(1 →, with branches attached at O-2,3 of 1,2,3,6-linked Glcp residues and terminal T-Glcp.
Current knowledge of the Escherichia coli phosphoenolpyruvate-carbohydrate phosphotransferase system: peculiarities of regulation and impact on growth and product formation.
Ecatepec, Mexico. In Appl Microbiol Biotechnol, 2012
We discuss three main approaches applied efficiently to avoid these constraints resulting in obtaining PTS(-) glc(+) mutants useful for production purposes: (1) adaptive selection in chemostat culture system of PTS(-) mutants, resulting in the selection of strains that recovered the ability to grow in glucose, along with the simultaneous consumption of two carbon sources and reduced acetate production; (2) replacement in PTS(-) strains of the native GalP promoter by strong promoters or the substitution of this permease by recombinant glucose transport system; and (3) enhancement of Crp (crp+) in mgsA, pgi, and ptsG mutants, resulting in derivative strains that abolished CCR, allowing the simultaneous consumption of mixtures of sugars with low acetate production.