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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Aug 2016.

GAL4 Gal4p

GAL4
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: CAN, ACID, V1a, HAD, OUT
Papers using GAL4 antibodies
A versatile molecular tagging method that targets proteins to avian reovirus protein muNS inclusions. Use in Protein Immobilization and Purification.
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Mitraki Anna, In PLoS ONE, 2009
... Monoclonal antibody specific for GAL4 DNA binding domain was obtained from Clontech (Saint Germain en Laye, ...
The eukaryotic translation elongation factor 1B{gamma} has a non-guanine nucleotide exchange factor role in protein metabolism.
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Preiss Thomas, In PLoS ONE, 2009
... GAL4 binding domain (BD) and used to screen a human skeletal muscle cDNA library (Clontech) as previously described ...
Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases
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O’Malley Bert W. et al., In Oncogene, 2008
... Yeast two-hybrid screening was performed by using Matchmaker Gal4 Two-Hybrid System 3 and a Matchmaker Pretransformed Normalized HeLa cDNA Library (Clontech Laboratories, Mountain View, CA) ...
Caveolin-1 activates Rab5 and enhances endocytosis through direct interaction.
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Holowka David, In PLoS ONE, 2008
... cDNA library (Matchmaker human keratinocyte library in pGAD10, Clontech) following the instructions of the Matchmaker Gal4 two-hybrid system (Clontech).
Systematic study of protein sumoylation: Development of a site-specific predictor of SUMOsp 2.0.
Supplier
Imhof Axel, In PLoS ONE, 2008
... Yeast-two-hybrid screen was performed using the MATCHMAKER GAL4 Two-Hybrid System 3 protocol (Clontech).
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Papers on GAL4
Adaptive and Background-Aware GAL4 Expression Enhancement of Co-registered Confocal Microscopy Images.
New
Bühler et al., Vienna, Austria. In Neuroinformatics, Feb 2016
UNASSIGNED: GAL4 gene expression imaging using confocal microscopy is a common and powerful technique used to study the nervous system of a model organism such as Drosophila melanogaster.
Attractant and repellent cues cooperate in guiding a subset of olfactory sensory axons to a well-defined protoglomerular target.
New
Raper et al., Philadelphia, United States. In Development, Feb 2016
A subset of olfactory sensory neurons are labeled by expression of the or111-7:IRES:GAL4 transgene whose axons terminate in the central zone (CZ) protoglomerulus.
The calcineurin inhibitor, Sarah/Nebula, exacerbates Aβ42 phenotypes in a Drosophila model of Alzheimer's disease.
New
Cho et al., Seoul, South Korea. In Dis Model Mech, Jan 2016
Interestingly, overexpression of sra using the UAS-GAL4 system exacerbated the rough eye phenotype, decreased survival rates, and increased neuronal cell death in Aβ42-expressing flies without modulating Aβ42 expression.
Transcriptional regulators form diverse groups with context-dependent regulatory functions.
New
Impact
Stark et al., Vienna, Austria. In Nature, Jan 2016
We recruited GAL4-DNA-binding-domain fusions of 812 Drosophila TFs and cofactors to 24 enhancer contexts and measured enhancer activities by 82,752 luciferase assays in S2 cells.
In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin.
New
Martin et al., Paris, France. In J Vis Exp, Dec 2015
In Drosophila, the GFP-aequorin gene has been placed under the control of the GAL4/UAS binary expression system allowing for targeted expression and imaging within the brain.
Selective and augmented β-glucuronidase expression combined with DOX-GA3 application elicits the potent suppression of prostate cancer.
New
Qin et al., Nanjing, China. In Oncol Rep, Dec 2015
Intravenous injection of a GAL4 regulated bicistronic adenovirus vector constructed to express βG under the control of the PSA promoter (Ad/PSAP‑GV16‑βG) and the application of DOX‑GA3 strongly inhibited tumor growth and prolonged the survival time of tumor‑bearing nude mice.
Differential pleiotropy and HOX functional organization.
Review
New
Percival-Smith et al., London, Canada. In Dev Biol, Mar 2015
This archetypic, modular view of transcription factor organization is based on the analyses of a few transcription factors such as GAL4, which may represent extreme exemplars rather than an archetype or the norm.
Transgenic fish systems and their application in ecotoxicology.
Review
New
Tyler et al., Exeter, United Kingdom. In Crit Rev Toxicol, Feb 2015
The GAL4-UAS system, where GAL4 is placed under the control of a desired promoter and UAS is fused with a fluorescent marker, has greatly enhanced model development for studies in ecotoxicology.
Chemical mutagens, transposons, and transgenes to interrogate gene function in Drosophila melanogaster.
Review
Bellen et al., United States. In Methods, 2014
Lastly, the ability to transform Drosophila with single copy transgenes using transposons or site-specific integration using the ΦC31 integrase has allowed numerous manipulations, including the ability to create and integrate genomic rescue constructs, generate duplications, RNAi knock-out technology, binary expression systems like the GAL4/UAS system as well as other methods.
Identification of a novel selective inverse agonist probe and analogs for the Retinoic acid receptor-related Orphan Receptor Gamma (RORγ)
Review
Griffin et al., Bethesda, United States. In Unknown Journal, 2012
In cotransfection assays, ML310 suppresses transcription activity in both GAL4-RORγ ligand binding domain (LBD) and full-length RORγ contexts.
Optical imaging for stem cell differentiation to neuronal lineage.
Review
Lee et al., Seoul, South Korea. In Nucl Med Mol Imaging (2010), 2012
The detection limit of weak promoters or reporter genes can be greatly enhanced by adopting a yeast GAL4 amplification system or an engineering-enhanced luciferase reporter gene.
Mediator acts upstream of the transcriptional activator Gal4.
GeneRIF
Lehming et al., Singapore, Singapore. In Plos Biol, 2011
Gal80 binds to the activation domain of Gal4 and prevents it from activating transcription.
Gal80 intersectional regulation of cell-type specific expression in vertebrates.
GeneRIF
Bonkowsky et al., Salt Lake City, United States. In Dev Dyn, 2011
These data demonstrate that Gal80 is a powerful addition to the genetic techniques available to map and manipulate neural circuits in zebrafish.
Absence of effects of Sir2 overexpression on lifespan in C. elegans and Drosophila.
Impact
Gems et al., London, United Kingdom. In Nature, 2011
A Drosophila strain with ubiquitous overexpression of dSir2 using the UAS-GAL4 system was long-lived relative to wild-type controls, as previously reported, but was not long-lived relative to the appropriate transgenic controls, and nor was a new line with stronger overexpression of dSir2.
MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes.
Impact
Bellen et al., Houston, United States. In Nat Methods, 2011
We can revert insertions that function as gene traps and cause mutant phenotypes to revert to wild type by RMCE and modify insertions to control GAL4 or QF overexpression systems or perform lineage analysis using the Flp recombinase system.
Galectin-4 functions as a tumor suppressor of human colorectal cancer.
GeneRIF
Rao et al., Amarillo, United States. In Int J Cancer, 2011
Loss of Galectin-4 is associated with colorectal cancer.
A versatile in vivo system for directed dissection of gene expression patterns.
Impact
Clandinin et al., Stanford, United States. In Nat Methods, 2011
Refining GAL4 expression patterns or independently manipulating multiple cell populations using additional binary systems are common experimental goals.
Galectins distinctively regulate central monocyte and macrophage function.
GeneRIF
Sturm et al., Berlin, Germany. In Cell Immunol, 2010
Data indicate that Gal-1, Gal-2, Gal-4, and partly Gal-3 bound to monocytes/macrophages.
Multiple metabolic signals influence GAL gene activation by modulating the interaction of Gal80p with the transcriptional activator Gal4p.
GeneRIF
Liu et al., Jinan, China. In Mol Microbiol, 2010
Overproduction of Gal3p or Gal4p mutants with altered Gal80p-binding characteristics restores GAL gene expression in mutants deficient in ATP or NADP(H) synthesis.
The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis.
Impact
Luo et al., Stanford, United States. In Cell, 2010
The Q system offers many applications, including (1) intersectional "logic gates" with the GAL4 system for manipulating transgene expression patterns, (2) GAL4-independent MARCM analysis, and (3) coupled MARCM analysis to independently visualize and genetically manipulate siblings from any cell division.
More papers using GAL4 antibodies
The kinase domain of mitochondrial PINK1 faces the cytoplasm.
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Cookson Mark R., In PLoS ONE, 2007
... hsp70-GAL4 [37] transgenic lines and the ...
Ligand-independent phosphorylation of Y869 (Y845) links mutant EGFR signaling to stat-mediated gene expression
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Parsons Sarah J. et al., In Oncogene, 2007
... Two hybrid screening was performed using MATCHMAKER GAL4 Two-Hybrid System 3 from Clontech (Palo Alto, CA) according ...
Nutritional control of protein biosynthetic capacity by insulin via Myc in Drosophila.
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Kapahi Pankaj, In PLoS Genetics, 2007
... UAS-sNPF, UAS-2XsNPF, UAS-sNPF-Ri, UAS-sNPFR1, UAS-sNPFR1-DN and sNPF-Gal4 transgenic flies were described in ...
GenBank.
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Unutmaz Derya, In PLoS ONE, 2007
... well as five caspases (C1, C2, C4, C5, C9) were cloned in frame with the GAL4 DNA-binding (pGBKT7, Clontech) as well as the ...
BAF180 is a critical regulator of p21 induction and a tumor suppressor mutated in breast cancer
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Kashanchi Fatah, In PLoS ONE, 2007
... A GAL4-based yeast two-hybrid assay was performed according to recommendations from BD Biosciences with a few exceptions ...
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.
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Tripp Ralph, In PLoS ONE, 2006
... GAL4 based Matchmaker (Clontech) yeast two-hybrid system was ...
A map of olfactory representation in the Drosophila mushroom body.
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Leal Walter S., In PLoS ONE, 2006
... dTdc2-Gal4 line [30], a second remobilized dTdc2-Gal4#1 line used as a transgenic insertion control ...
Activity- and mTOR-dependent suppression of Kv1.1 channel mRNA translation in dendrites
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Passos-Bueno Maria Rita et al., In BMC Research Notes, 2005
... Yeast two-hybrid screening was conducted using Matchmaker GAL4 two-hybrid system 3 (Clontech, BD Biosciences) ...
Crystal Structure and Interactions of the PAS Repeat Region of the Drosophila Clock Protein PERIOD.
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Kobe Bostjan, In PLoS ONE, 2004
... MATa, ura3-52, his3-200, ade2-101, lys2-801, leu2-3, 112, trp1-901, tyr1-501, gal4-D512, gal80-D538, ade5::hisG) of Saccharomyces cerevisiae YM4271 (Matchmaker One-Hybrid System, Clontech, Palo Alto, CA) to ...
A potent small molecule inhibits polyglutamine aggregation in Huntington’s disease neurons and suppresses neurodegeneration in vivo
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Yamaguchi Masamitsu et al., In Journal of Biomedicine and Biotechnology, 2004
... The transgenic fly line carrying GMR-GAL4 on X chromosome was described earlier [22] as was the transgenic fly line carrying gmr-Q92 ...
Comparative analysis of Corazonin-encoding genes (Crz's) in Drosophila species and functional insights into Crz-expressing neurons.
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McCabe Brian D., In PLoS ONE, 2004
... We used Canton S for a wild type stock; GAL4 transgenic lines as described in ...
Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina
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Fjose Anders et al., In BMC Neuroscience, 2004
... Zebrafish genomic sequences flanking the Tol2 vector insertions associated with retinal expression of Gal4-VP16/eGFP in the two transgenic lines ...
The cyclin A1-CDK2 complex regulates DNA double-strand break repair.
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Bergmann Andreas, In PLoS ONE, 2003
... To identify INCA1-interacting proteins, a yeast two-hybrid (Y2H) screen was performed using the Matchmaker™ Gal4 two-hybrid system 3 (Clontech).
Small GTPases in vesicle trafficking.
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Hassan Bassem A., In PLoS ONE, 2003
... -Gal4 transgenic flies ...
Identification of a novel isoform of Cdk9.
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Zeleznik-Le Nancy, In PLoS Biology, 2002
... NM_001241), and Dot1l (accession number NM_199322) were introduced into the vectors pGADT7 or pACT2 as GAL4-activation fusions and into pGBKT7 or pAS2-1 as bait fusions (all vectors from Clontech).
Mutations in sticky lead to defective organization of the contractile ring during cytokinesis and are enhanced by Rho and suppressed by Rac
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Glover David M. et al., In The Journal of Cell Biology, 2002
... ey-GAL4 (Bonini et al., 1997) and UAS-RacGAP50C.dsRNA (Billuart et al., 2001) transgenic fly stocks were obtained ...
Genetic control of cuticle formation during embryonic development of Drosophila melanogaster.
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Lewin Alfred, In PLoS ONE, 2001
...  = GAL4-da.G32}.To generate a small deletion, which removes most of the mbl ...
Nesprin-3, a novel outer nuclear membrane protein, associates with the cytoskeletal linker protein plectin
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Sonnenberg Arnoud et al., In The Journal of Cell Biology, 2001
... Gal4 transcriptional activation domain (AD) expression vector pGAD10 (CLONTECH Laboratories, Inc.) was performed ...
Kazrin, a novel periplakin-interacting protein associated with desmosomes and the keratinocyte plasma membrane
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Watt Fiona M. et al., In The Journal of Cell Biology, 2001
... Yeast two-hybrid protein interaction experiments and library screenings were performed using the MATCHMAKER GAL4 two-hybrid system 3 (BD Biosciences) according to the manufacturer's ...
Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins
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Jockusch Brigitte M. et al., In The Journal of Cell Biology, 2000
... to screen the mouse embryonic (E17.5) and a human heart two-hybrid library, both fused to the GAL4 transcription-activating domain (CLONTECH).
DA-Raf1, a competent intrinsic dominant-negative antagonist of the Ras–ERK pathway, is required for myogenic differentiation
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Endo Takeshi et al., In The Journal of Cell Biology, 1999
... Gal4 DNA-binding domain in pGBD9 vector (CLONTECH Laboratories, Inc.) ...
Different splice variants of filamin-B affect myogenesis, subcellular distribution, and determine binding to integrin β subunits
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Sonnenberg Arnoud et al., In The Journal of Cell Biology, 1999
... anti-paxillin (349) and anti-phosphotyrosine (PY20) were from Transduction Laboratories; mouse anti–Gal4 binding domain (BD), mouse anti–Gal4 activation domain (AD), and rabbit anti-HA antibodies (sc-510, sc-1663, and sc-805, respectively) were from Santa Cruz Biotechnology, Inc.; mouse anti-HA (12CA5) ...
Fast synaptic fatigue in shibire mutants reveals a rapid requirement for dynamin in synaptic vesicle membrane trafficking.
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Zars Troy, In PLoS ONE, 1999
... The glial-specific GAL4 driver line, dEAAT1-GAL4, and the dEAAT1 RNAi transgenic line, UAS-dEAAT1-IR-II ...
Stat3 regulates microtubules by antagonizing the depolymerization activity of stathmin
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Cao Xinmin et al., In The Journal of Cell Biology, 1999
... was cloned into the pGBKT7 plasmid containing the GAL4 DNA-binding domain and was used as bait in a yeast two-hybrid screen of a fetal mouse brain cDNA library (CLONTECH Laboratories, Inc.) as previously ...
The role of cell death in regulating the size and shape of the mammalian forebrain.
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Zou Hui, In PLoS ONE, 1998
... For yeast two-hybrid analysis, we used the MATCHMAKER GAL4 Two-Hybrid System 3 (Clontech Laboratories) and ...
A kinase-deficient TrkC receptor isoform activates Arf6–Rac1 signaling through the scaffold protein tamalin
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Tessarollo Lino et al., In The Journal of Cell Biology, 1998
... fusion bait plasmid and an adult mouse brain cDNA library fused in frame to the GAL4 activation domain (Mouse Brain MATCHMAKER cDNA Library; CLONTECH Laboratories, Inc.) were sequentially ...
Pleiotropic control of glucose and hormone responses by PRL1, a nuclear WD protein, in Arabidopsis.
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Uhrig Joachim F et al., In Genome Biology, 1997
... ] encodes the full-length, wild-type Gal4 protein under control of the ADH promoter and was obtained from Clontech.
Estrogen receptor activation function 1 works by binding p160 coactivator proteins
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Kushner Peter J et al., In Nuclear Receptor, 1997
... SalI sites, the PCR fragment was digested with these enzymes and subcloned into the pM GAL4 expression vector (Clontech Laboratories, Inc ...
Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cells
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Kuriyama Ryoko et al., In The Journal of Cell Biology, 1997
... Aurora-A was fused in-frame to the GAL4 DNA-binding domain using the pGBT vector (CLONTECH Laboratories, Inc.) ...
Molecular interactions in cell adhesion complexes
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Aota Shin-ichi et al., In The Journal of Cell Biology, 1996
... ) fused with the GAL4 DNA-binding domain of pGBT9 (Clontech ...
In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector.
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Ambrose Zandrea, In PLoS ONE, 1995
... For the construction of the inducible vectors (pH-TRE-Gal4, TRE-PR/Gal4 and pH-TRE-PRm/Gal4), a 7X Tet-Responsive Element (TRE) was amplified from the pTRE-tight (Clontech) with the forward primer ...
Desmosomal cadherin binding domains of plakoglobin
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Vinzens Ute et al., In The Journal of Cell Biology, 1995
... Vectors for the expression of GAL4 fusion proteins in yeast were the pBD and pAD vectors (Stratagene) and the pAS2-1 and pGAD424 vectors (CLONTECH Laboratories) ...
Characterization of myotonin dystrophy kinase (DMK) protein in human and rodent muscle and central nervous tissue
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Ohno Shigeo et al., In The Journal of Cell Biology, 1994
... ) was cloned in-frame with the GAL4-DNA–binding domain into pGBT9 (CLONTECH ...
Dissociation of the complex of dystrophin and its associated proteins into several unique groups by n-octyl beta-D-glucoside
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Kunkel Louis M. et al., In The Journal of Cell Biology, 1993
... human skeletal muscle total RNA with primers containing EcoRI linkers, and inserted downstream of the Gal4 DNA-binding domain in the (bait) vector pGBT9 (CLONTECH Laboratories) ...
Measurement of co-localization of objects in dual color confocal images.
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Buday Laszlo, In PLoS ONE, 1992
... inserted between EcoRI and SalI sites in-frame with the DBD of the yeast transcription factor GAL4, in pGBKT7 plasmid (Clontech, Mountain View, CA) ...
Monoclonal antibody 7H6 reacts with a novel tight junction-associated protein distinct from ZO-1, cingulin, and ZO-2
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Bronstein Jeff M. et al., In The Journal of Cell Biology, 1992
... GAL4 activation domain cDNA fusion libraries were constructed in modified pGAD GH vector (CLONTECH, Laboratories, Inc.) using mRNA ...
Production of monoclonal antibody that recognizes glycogen and its application for immunohistochemistry
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Juhász Gábor et al., In Autophagy, 1992
... hs-Gal4 (Bloomington Drosophila Stock Center, BDSC), and transgenic RNAi lines for ...
The discs-large tumor suppressor gene of drosophila encodes a guanylate kinase homolog localized at septate junctions
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Sheng Morgan et al., In The Journal of Cell Biology, 1990
... fusion vector) and used to screen rat and human brain cDNA libraries constructed in pGAD10 (GAL4 activation domain vector, Clontech, Palo Alto, CA) ...
An electrotransfection method for yeast-two-hybrid screening
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Grant Seth G. N, In PLoS Biology, 1985
... Library screening was done with a rat brain cDNA library in pACT2 (Matchmaker-GAL4 Two-Hybrid II; Clontech).
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