Transcriptional profiling of human epithelial cells infected with plasmid-bearing and plasmid-deficient Chlamydia trachomatis.
Hamilton, United States. In Infect Immun, 17 Dec 2014
Infection of human epithelial cells with either strain increased the expression of host genes coding for pro-inflammatory (GM-CSF, MCSF, IL6, IL8, IL1A, CXCL1, CXCL2, CXCL3, ICAM1), chemo-attraction (CCL20, CCL5, CXCL10), immune suppression (PD-L1, NFKB1B, TNFAIP3, CGB), apoptosis (CASP9, FAS, IL24), cell growth and fibrosis (EGR1 and IL20) proteins.
Silica Nanoparticles Induced Metabolic Stress through EGR1, CCND, and E2F1 Genes in Human Mesenchymal Stem Cells.
Riyadh, Saudi Arabia. In Appl Biochem Biotechnol, 06 Dec 2014
In the present study, we examined the structural characteristics of these nano-sized silica particles adopting SEM and dynamic light scattering (DLS) and assessed the alterations in the cell cycle induced by these silica particles in human mesenchymal stem cells (hMSCs) adopting 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay, morphological changes in the cells adopting fluorescent microscopy, cell cycle analysis adopting flow cytometry, and the expression of genes linked to cell cycle (i.e., proliferating cell nuclear antigen (PCNA), early growth response protein (EGR1), E2F transcription factor (E2F1), cyclin D1, cyclin C, and cyclin D3) adopting qPCR.