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Dynactin 2

dynamitin, p50 dynamitin
This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: dynactin, CD13, CAN, Actin, V1a
Papers on dynamitin
Acrylamide Retards the Slow Axonal Transport of Neurofilaments in Rat Cultured Dorsal Root Ganglia Neurons and the Corresponding Mechanisms.
New
Xie et al., Jinan, China. In Neurochem Res, Jan 2016
Then for the underlying mechanisms exploration, the protein level of neurofilament subunits, motor proteins kinesin and dynein, and dynamitin subunit of dynactin in DRG neurons were assessed by western blotting and the concentrations of ATP was detected using ATP Assay Kit.
Chlamydia trachomatis inclusion membrane protein CT850 interacts with the dynein light chain DYNLT1 (Tctex1).
New
Hackstadt et al., Hamilton, United States. In Biochem Biophys Res Commun, Jul 2015
Unlike most substrates of the dynein motor, disruption of the dynactin cargo-linking complex by over-expression of the p50 dynamitin subunit does not inhibit C. trachomatis transport.
Dynamitin affects cell-surface expression of voltage-gated sodium channel Nav1.5.
Le Bouffant et al., In Biochem J, 2014
Using a yeast two-hybrid screen, we identified dynamitin as a Nav1.5-interacting
Cytoplasmic dynein promotes HIV-1 uncoating.
Berthoux et al., Trois-Rivières, Canada. In Viruses, 2014
To interfere with dynein function, we depleted dynein heavy chain (DHC) using RNA interference, and we over-expressed p50/dynamitin.
Enhancement of dynein-mediated autophagosome trafficking and autophagy maturation by ROS in mouse coronary arterial myocytes.
Li et al., Richmond, United States. In J Cell Mol Med, 2014
Inhibition of dynein activity by (erythro-9-(2-hydroxy-3-nonyl)adenine) (EHNA) or disruption of the dynein complex by dynamitin (DCTN2) overexpression blocked ROS-induced dynein activation, AP movement and APL formation, and resulted in an accumulation of AP along with a failed breakdown of AP.
Dynactin integrity depends upon direct binding of dynamitin to Arp1.
Schroer et al., Baltimore, United States. In Mol Biol Cell, 2014
The dynactin subunit dynamitin (also known as p50) is believed to be integral to dynactin structure because free dynamitin displaces the dynein-binding p150(Glued) subunit from the cargo-binding Arp1 filament.
Interaction of foot-and-mouth disease virus nonstructural protein 3A with host protein DCTN3 is important for viral virulence in cattle.
Borca et al., New York City, United States. In J Virol, 2014
Overexpression of DCTN3 or proteins known to disrupt dynein, p150/Glued and 50/dynamitin, resulted in decreased FMDV replication in infected cells.
Involvement of microtubular network and its motors in productive endocytic trafficking of mouse polyomavirus.
Forstova et al., Praha, Czech Republic. In Plos One, 2013
Inhibition of cytoplasmic dynein-based motility by overexpression of dynamitin affected perinuclear translocation of the virus, delivery of virions to the ER and substantially reduced the numbers of infected cells, while overexpression of dominant-negative form of kinesin-1 or kinesin-2 had no significant impact on virus localization and infectivity.
Prenatal alcohol exposure modifies glucocorticoid receptor subcellular distribution in the medial prefrontal cortex and impairs frontal cortex-dependent learning.
Caldwell et al., Albuquerque, United States. In Plos One, 2013
The levels of critical GR trafficking proteins, FKBP51, Hsp90, cyclophilin 40, dynamitin and dynein intermediate chain, were altered in PAE mice, in favor of the exclusion of GR from the nucleus, indicating dysregulation of GR trafficking.
Disruption of the dynein-dynactin complex unveils motor-specific functions in osteoclast formation and bone resorption.
Pavlos et al., Australia. In J Bone Miner Res, 2013
Unexpectedly, disruption of the dynein-dynactin complex by exogenous p50/dynamitin expression retards osteoclast formation in vitro, owing largely to prolonged mitotic stasis of osteoclast progenitor cells.
Evolution of the eukaryotic dynactin complex, the activator of cytoplasmic dynein.
Kollmar et al., Göttingen, Germany. In Bmc Evol Biol, 2011
It is composed of eleven different polypeptides of which eight are unique to this complex, namely dynactin1 (p150(Glued)), dynactin2 (p50 or dynamitin), dynactin3 (p24), dynactin4 (p62), dynactin5 (p25), dynactin6 (p27), and the actin-related proteins Arp1 and Arp10 (Arp11).
The association of viral proteins with host cell dynein components during virus infection.
Review
Rodríguez-Crespo et al., Madrid, Spain. In Febs J, 2011
Furthermore, overexpression of p50 dynamitin, which blocks the dynein-dynactin interaction, or incubation of infected cells with peptides that compete with viral polypeptides for dynein binding have been shown to alter the viral retrograde transport.
Molecular and functional basis for the scaffolding role of the p50/dynamitin subunit of the microtubule-associated dynactin complex.
GeneRIF
Benichou et al., Paris, France. In J Biol Chem, 2010
the determinants of p50/DM required for self-oligomerization of the protein and for interactions with other subunits of the dynactin complex
N-terminal region of ZW10 serves not only as a determinant for localization but also as a link with dynein function.
GeneRIF
Tagaya et al., Hachiōji, Japan. In Genes Cells, 2008
The interaction between ZW10 and dynamitin showed that the N-terminal region of ZW10 is the major binding site for dynamitin and, like full-length ZW10, could move along microtubules to the centrosomal area in a dynein-dynactin-dependent manner.
Dynamitin mutagenesis reveals protein-protein interactions important for dynactin structure.
GeneRIF
Schroer et al., Baltimore, United States. In Traffic, 2008
Data show how dynamitin's different structural domains contribute to its ability to self-associate, interact with dynactin and assemble into a complex with its close binding partner, p24.
Phenotypic changes associated with DYNACTIN-2 (DCTN2) over expression characterise SJSA-1 osteosarcoma cells.
GeneRIF
Mighell et al., Leeds, United Kingdom. In Mol Carcinog, 2006
study does not support a major role for DCTN2 overexpression in carcinogenesis
Requirement for microtubules and dynein motors in the earliest stages of peroxisome biogenesis.
GeneRIF
Walton et al., London, Canada. In Traffic, 2005
DCTN2 is required in the earliest stages of peroxisome biogenesis.
Kinetochore dynein: its dynamics and role in the transport of the Rough deal checkpoint protein.
Impact
Hays et al., Blacksburg, United States. In Nat Cell Biol, 2001
A functional conjugate of dynamitin with green fluorescent protein accumulates rapidly at prometaphase kinetochores, and subsequently migrates off kinetochores towards the poles during late prometaphase and metaphase.
p53 is associated with cellular microtubules and is transported to the nucleus by dynein.
Impact
Fojo et al., Bethesda, United States. In Nat Cell Biol, 2000
Overexpression of dynamitin or microinjection of anti-dynein antibody before DNA damage abrogates nuclear accumulation of p53.
The role of the dynactin complex in intracellular motility.
Review
Holzbaur et al., Philadelphia, United States. In Int Rev Cytol, 1997
Studies on dynamitin, the 50-kDa dynactin subunit, predict a role for dynactin in mitotic spindle assembly.
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