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proteins. Page last changed on 19 Dec 2016.
DNase I, Deoxyribonuclease I, Deoxyribonuclease
This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008] (from
... central placental cotyledons was minced, trypsinized (0.05% trypsin-EDTA solution; Invitrogen), and treated with 10 U/ml DNase I (Sigma-Aldrich) in Dulbecco's modified Eagle's medium (DMEM; Gibco) at 37°C for 5 min several times, and finally filtered through a cell strainer (BD Biosciences).
Lava et al., Switzerland. In Pediatr Pulmonol, Feb 2016
Finally, stimulating observations suggest that magnesium supplements might achieve an improvement in respiratory muscle strength and mucolytic activity of both recombinant and endogenous deoxyribonuclease.
Lis et al., Buffalo, United States. In Infect Immun, Feb 2016
Uric acid levels in the gut lumen increased in response to exogenous DNA and these increases were enhanced by the actions of DNase I. Interestingly, addition of DNase I reduced the numbers of EPEC bacteria recovered after a 20 h infection and protected against EPEC-induced histologic damage.
Mill et al., Colchester, United Kingdom. In Epigenetics, Feb 2016
We identify ASM flanking known imprinted regions, and show that ASM sites are enriched in DNase I hypersensitivity sites and often located in an extended genomic context of intermediate DNA methylation.
Though deoxyribonuclease I (DNase I) was used to probe the structure of nucleosomes in the 1960s and 70s, in the current high-throughput sequencing era, it has mainly been used to study genomic regions devoid of nucleosomes.
Hager et al., Bethesda, United States. In Nucleus, Feb 2016
Using glucocorticoid receptor (GR) as a model system we will discuss spatiotemporal relationships between receptor/chromatin interactions, lifetimes of the DNase I hypersensitivity sites (DHSs), long-range interactions, and gene regulation.
Stamatoyannopoulos et al., Seattle, United States. In Nat Genet, Dec 2015
We use allelically resolved genomic DNase I footprinting data encompassing 166 individuals and 114 cell types to identify >60,000 common variants that directly influence transcription factor occupancy and regulatory DNA accessibility in vivo.
Seldin, Davis, United States. In J Autoimmun, Nov 2015
These studies to identify genetic risk loci are also complemented by progress in gene expression studies including definition of expression quantitative trait loci (eQTL), various alterations in chromatin structure including histone marks, DNase I sensitivity, repressed chromatin regions as well as transcript factor binding sites.
Hakonarson et al., Philadelphia, United States. In Nat Med, Sep 2015
The pAID-associated single-nucleotide polymorphisms (SNPs) were functionally enriched for deoxyribonuclease (DNase)-hypersensitivity sites, expression quantitative trait loci (eQTLs), microRNA (miRNA)-binding sites and coding variants.
Beer et al., Baltimore, United States. In Nat Genet, Aug 2015
We show that deltaSVM accurately predicts the impact of SNPs on DNase I sensitivity in their native genomic contexts and accurately predicts the results of dense mutagenesis of several enhancers in reporter assays.
Spencer et al., London, United Kingdom. In Cochrane Database Syst Rev, 2014
There was inconclusive evidence on the use of long-term antibiotics and nebulised hypertonic saline for reducing exacerbation frequency and evidence that human deoxyribonuclease (RhDNase) increases exacerbation frequency.
Mortensen et al., Tromsø, Norway. In Plos One, 2011
silencing of renal DNaseI gene expression initiates a cascade of inflammatory signals including activation of Toll like receptors and Clec4e, leading to progression of both murine and human lupus nephritis
Takeshita et al., Izumo, Japan. In Dna Cell Biol, 2011
The genetic aspects of DNASE1 with regard to all the SNPs in wide-ranging ethnic groups could be first demonstrated. Further, there was no correlation of all the polymorphic SNPs other than nonsynonymous ones with serum DNaseI activity levels.
Kluytmans J. A. J. W. et al., In European Journal of Clinical Microbiology & Infectious Diseases, 1980
... All isolates with discordant test results of the DML Staph assay were retested for deoxyribonuclease (DNAse) activity (in-house product) , coagulase activity (Coagulase Plasma Rabbit, Becton Dickinson, Bedford, MA, USA), the ...