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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Aug 2016.

Ligase IV, DNA, ATP-dependent

DNA repair enzyme, DNA ligase IV, LIG4
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: CAN, XRCC4, POLYMERASE, HAD, V1a
Papers on DNA repair enzyme
Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.
New
Zhang et al., Beijing, China. In Proc Natl Acad Sci U S A, Feb 2016
Surprisingly, in cells deficient for core classic NHEJ factors such as DNA ligase IV (Lig4), substantial end-joining activities have been observed in various situations, suggesting the existence of alternative end-joining (A-EJ) activities.
Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination.
New
Yu et al., East Lansing, United States. In Proc Natl Acad Sci U S A, Feb 2016
Deletion of DNA ligase IV (Lig4), a core component of the NHEJ pathway, reduces CSR efficiency in a mouse B-cell line capable of robust cytokine-stimulated CSR in cell culture.
Next generation sequencing revealed DNA ligase IV deficiency in a "developmentally normal" patient with massive brain Epstein-Barr virus-positive diffuse large B-cell lymphoma.
New
Aleinikova et al., Minsk, Belarus. In Clin Immunol, Feb 2016
INTRODUCTION: Here we present an unusual case of DNA ligase IV deficiency syndrome without dysmorphic facial findings and microcephaly complicated with Epstein-Barr virus-associated large B-cell lymphoma with the right lung involvement and a massive brain tumor lesion in a two-year-old female.
Microbial production of dihomo-γ-linolenic acid by Δ5-desaturase gene-disruptants of Mortierella alpina 1S-4.
New
Ogawa et al., Kyoto, Japan. In J Biosci Bioeng, Feb 2016
In previous study, we had already identified and disrupted the lig4 gene encoding DNA ligase 4, which involves in non-homologous end joining, in M. alpina 1S-4, and the Δlig4 strain had showed efficient gene-targeting.
Molecular and Immunological Characterization of DNA ligase IV deficiency.
New
Zhao et al., Chongqing, China. In Clin Immunol, Feb 2016
UNASSIGNED: DNA ligase IV (LIG4) deficiency is an extremely rare autosomal recessive primary immunodeficiency disease caused by the LIG4 mutation.
Non-homologous functions of the AlkB homologs.
Review
New
Larsen et al., Oslo, Norway. In J Mol Cell Biol, Dec 2015
The DNA repair enzyme AlkB was identified in E. coli more than three decades ago.
Inhibition of nonhomologous end joining to increase the specificity of CRISPR/Cas9 genome editing.
Review
New
Raghavan et al., Bengaluru, India. In Febs J, Nov 2015
SCR7, a DNA ligase IV inhibitor, was recently identified and characterized as a potential anticancer compound.
Toward an effective strategy in glioblastoma treatment. Part I: resistance mechanisms and strategies to overcome resistance of glioblastoma to temozolomide.
Review
New
Lagarce et al., Angers, France. In Drug Discov Today, Jul 2015
This resistance is caused by DNA repair enzyme activity, overexpression of epidermal growth factor receptor (EGFR), galectin-1, murine double minute 2 (Mdm2), p53 and phosphatase and tensin homolog (PTEN) mutations.
Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
New
Impact
Kühn et al., Berlin, Germany. In Nat Biotechnol, May 2015
To enhance HDR, enabling the insertion of precise genetic modifications, we suppressed the NHEJ key molecules KU70, KU80 or DNA ligase IV by gene silencing, the ligase IV inhibitor SCR7 or the coexpression of adenovirus 4 E1B55K and E4orf6 proteins in a 'traffic light' and other reporter systems.
Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining.
New
Impact
Ploegh et al., Cambridge, United States. In Nat Biotechnol, May 2015
To promote HDR at the expense of NHEJ, we targeted DNA ligase IV, a key enzyme in the NHEJ pathway, using the inhibitor Scr7.
QM and QM/MM Methods Compared: Case Studies on Reaction Mechanisms of Metalloenzymes.
Review
Szaleniec et al., Kraków, Poland. In Adv Protein Chem Struct Biol, 2014
The review is illustrated by case studies for isopenicillin N synthase, ethylbenzene dehydrogenase, cytochrome P450 enzyme, AlkB DNA repair enzyme as well as 4-hydroxyphenylpyruvate dioxygenase.
MutY-glycosylase: an overview on mutagenesis and activities beyond the GO system.
Review
Agnez-Lima et al., Natal, Brazil. In Mutat Res, 2014
MutY has been described as a DNA repair enzyme in the GO system responsible for removing adenine residues misincorporated in 8-oxoG:A mispairs, avoiding G:C to T:A mutations.
Inactivation of yeast Isw2 chromatin remodeling enzyme mimics longevity effect of calorie restriction via induction of genotoxic stress response.
Impact
Berger et al., Philadelphia, United States. In Cell Metab, 2014
In particular, isw2Δ cells show an increased response to genotoxic stresses, and the DNA repair enzyme Rad51 is important for isw2Δ-mediated longevity.
An inhibitor of nonhomologous end-joining abrogates double-strand break repair and impedes cancer progression.
Impact
Raghavan et al., Bengaluru, India. In Cell, 2013
DNA Ligase IV is responsible for sealing of double-strand breaks (DSBs) during nonhomologous end-joining (NHEJ).
The α2 helix in the DNA ligase IV BRCT-1 domain is required for targeted degradation of ligase IV during adenovirus infection.
GeneRIF
Hanakahi et al., Baltimore, United States. In Virology, 2012
The alpha2 helix in the Lig4 BRCT-1 domain is required for adenovirus-mediated degradation of Lig4.
Structural insights into the role of domain flexibility in human DNA ligase IV.
GeneRIF
Blundell et al., Cambridge, United Kingdom. In Structure, 2012
The flexibility of the DNA ligase IV catalytic region is limited in a manner that affects the formation of the LigIV/XRCC4/XLF-Cernunnos complex.
DNA repair gene polymorphisms and risk of early onset colorectal cancer in Lynch syndrome.
GeneRIF
Scott et al., Newcastle, Australia. In Cancer Epidemiol, 2012
polymorphisms in LIG4 do not contribute to cancer risk in a population of Lynch syndrome patients with colorectal cancer
X-ray repair cross-complementing protein 1 (XRCC1) deficiency enhances class switch recombination and is permissive for alternative end joining.
GeneRIF
Yu et al., East Lansing, United States. In Proc Natl Acad Sci U S A, 2012
Lig4 and XRCC1 double-deficient cells switch as efficiently as Lig4-deficient cells, clearly indicating that XRCC1 is dispensable for A-EJ in CH12F3 cells during class switch recombination
XRCC4 controls nuclear import and distribution of Ligase IV and exchanges faster at damaged DNA in complex with Ligase IV.
GeneRIF
Mielke et al., Düsseldorf, Germany. In Dna Repair (amst), 2012
XRCC4 modulates the dynamic interaction of the Ligase IV/XRCC4 complex with the NHEJ machinery at double-stranded DNA breaks
Thymine DNA glycosylase is essential for active DNA demethylation by linked deamination-base excision repair.
Impact
Bellacosa et al., Philadelphia, United States. In Cell, 2011
Here, we show that either knockout or catalytic inactivation of the DNA repair enzyme thymine DNA glycosylase (TDG) leads to embryonic lethality in mice.
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