Enzymes involved in organellar DNA replication in photosynthetic eukaryotes.
Tokyo, Japan. In Front Plant Sci, 2013
Although the replication mechanisms of these organellar genomes remain unclear in photosynthetic eukaryotes, several organelle-localized enzymes related to genome replication, including DNA polymerase, DNA primase, DNA helicase, DNA topoisomerase, single-stranded DNA maintenance protein, DNA ligase, primer removal enzyme, and several DNA recombination-related enzymes, have been identified.
Monitoring regulation of DNA repair activities of cultured cells in-gel using the comet assay.
Liverpool, United Kingdom. In Front Genet, 2013
For example, we have shown that the E3 ubiquitin ligase Mule, the tumor suppressor protein ARF, and the deubiquitylation enzyme USP47 modulate DNA repair by controlling cellular levels of DNA polymerase β, and also that polynucleotide kinase phosphatase levels are controlled by ATM-dependant phosphorylation and Cul4A-DDB1-STRAP-dependent ubiquitylation.
Magic spots cast a spell on DNA primase.
Madison, United States. In Cell, 2007
In this issue, Wang et al. (2007) show that (p)ppGpp also inhibits DNA replication elongation by interfering with DNA primase activity.
DNA primase acts as a molecular brake in DNA replication.
Boston, United States. In Nature, 2006
A hallmark feature of DNA replication is the coordination between the continuous polymerization of nucleotides on the leading strand and the discontinuous synthesis of DNA on the lagging strand.
Replisome-mediated DNA replication.
United States. In Annu Rev Biochem, 2000
The components of the replication complex can be grouped into functional categories as follows: DNA polymerase, helix destabilizing protein, polymerase accessory factors, and primosome (DNA helicase and DNA primase activities).