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Ligase I, DNA, ATP-dependent

DNA ligase, DNA ligase I
LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: CAN, POLYMERASE, XRCC4, V1a, STEP
Papers using DNA ligase antibodies
Cyclophilin E functions as a negative regulator to influenza virus replication by impairing the formation of the viral ribonucleoprotein complex.
Supplier
Jin Dong-Yan, In PLoS ONE, 2010
... Other suppliers were: DNA polymerase, T4 DNA ligase and restriction enzymes (New England Biolabs); pET30a vector (Novagen); pGEX-6p-1 ...
A highly parallel method for synthesizing DNA repeats enables the discovery of 'smart' protein polymers
Supplier
Kouprina Natalay et al., In Biological Procedures Online, 2010
... T4 DNA Ligase (New England Biolabs, cat ...
Development of highly-sensitive microbial biosensors by mutation of the nahR regulatory gene.
Supplier
Doering Christopher B., In PLoS ONE, 2009
... Restriction enzymes were purchased from Fermentas and T4 DNA ligase was purchased from New England Biolabs.
Computational analysis of off-rate selection experiments to optimize affinity maturation by directed evolution
Supplier
McCafferty John et al., In Analytical Biochemistry, 2009
... , 1 mM ATP, 10 mM DTT, and T4 DNA ligase (1000 U, New England Biolabs) in a total volume of ...
Sequence-specific random coil chemical shifts of intrinsically disordered proteins.
Supplier
Neyrolles Olivier, In PLoS ONE, 2009
... Restriction enzymes, T4 DNA ligase and other molecular biology reagents were purchased from New England Biolabs, Roche or Promega ...
more suppliers
Papers on DNA ligase
Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.
New
Zhang et al., Beijing, China. In Proc Natl Acad Sci U S A, Feb 2016
By using a clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system, we generated mouse CH12F3 cell lines in which, in addition to Lig4, either Lig1 or nuclear Lig3, representing the cells containing a single DNA ligase (Lig3 or Lig1, respectively) in their nucleus, was completely ablated.
Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination.
New
Yu et al., East Lansing, United States. In Proc Natl Acad Sci U S A, Feb 2016
Deletion of DNA ligase IV (Lig4), a core component of the NHEJ pathway, reduces CSR efficiency in a mouse B-cell line capable of robust cytokine-stimulated CSR in cell culture.
Bacterial psychrophilic ligA alleles that confer temperature-sensitivity upon substitution into mesophilic bacteria and yeast.
New
Nano et al., Victoria, Canada. In Appl Environ Microbiol, Feb 2016
We also tested two ligA alleles from two Pseudoalteromonas strains for their ability to support the viability of a Saccharomyces cerevisiae strain that lacked its essential gene, CDC9, encoding an ATP-dependent DNA ligase.
Next generation sequencing revealed DNA ligase IV deficiency in a "developmentally normal" patient with massive brain Epstein-Barr virus-positive diffuse large B-cell lymphoma.
New
Aleinikova et al., Minsk, Belarus. In Clin Immunol, Feb 2016
INTRODUCTION: Here we present an unusual case of DNA ligase IV deficiency syndrome without dysmorphic facial findings and microcephaly complicated with Epstein-Barr virus-associated large B-cell lymphoma with the right lung involvement and a massive brain tumor lesion in a two-year-old female.
Microbial production of dihomo-γ-linolenic acid by Δ5-desaturase gene-disruptants of Mortierella alpina 1S-4.
New
Ogawa et al., Kyoto, Japan. In J Biosci Bioeng, Feb 2016
In previous study, we had already identified and disrupted the lig4 gene encoding DNA ligase 4, which involves in non-homologous end joining, in M. alpina 1S-4, and the Δlig4 strain had showed efficient gene-targeting.
Reprint of "Oxidant and environmental toxicant-induced effects compromise DNA ligation during base excision DNA repair".
Review
New
Wilson et al., United States. In Dna Repair (amst), Dec 2015
The conformational differences in DNA polymerase β (pol β) associated with incorrect or oxidized nucleotide (8-oxodGMP) insertion could impact channeling of the repair intermediate to the final step of BER, i.e., DNA ligation by DNA ligase I or the DNA Ligase III/XRCC1 complex.
Oxidant and environmental toxicant-induced effects compromise DNA ligation during base excision DNA repair.
Review
New
Wilson et al., United States. In Dna Repair (amst), Nov 2015
The conformational differences in DNA polymerase β (pol β) associated with incorrect or oxidized nucleotide (8-oxodGMP) insertion could impact channeling of the repair intermediate to the final step of BER, i.e., DNA ligation by DNA ligase I or the DNA Ligase III/XRCC1 complex.
Why Johnny can't clone: Common pitfalls and not so common solutions.
Review
New
Matsumura, Atlanta, United States. In Biotechniques, Sep 2015
In particular, the virtues of agarose gel electrophoresis monitoring, the nature of the interactions between DNA and silica, and challenges associated with thermostable DNA polymerases, restriction endonucleases, and T4 DNA ligase are explored.
Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
New
Impact
Kühn et al., Berlin, Germany. In Nat Biotechnol, May 2015
To enhance HDR, enabling the insertion of precise genetic modifications, we suppressed the NHEJ key molecules KU70, KU80 or DNA ligase IV by gene silencing, the ligase IV inhibitor SCR7 or the coexpression of adenovirus 4 E1B55K and E4orf6 proteins in a 'traffic light' and other reporter systems.
Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining.
New
Impact
Ploegh et al., Cambridge, United States. In Nat Biotechnol, May 2015
To promote HDR at the expense of NHEJ, we targeted DNA ligase IV, a key enzyme in the NHEJ pathway, using the inhibitor Scr7.
From Structure-Function Analyses to Protein Engineering for Practical Applications of DNA Ligase.
Review
Nishida et al., Kokubunji, Japan. In Archaea, 2014
DNA ligases are indispensable in all living cells and ubiquitous in all organs.
Archaeal Nucleic Acid Ligases and Their Potential in Biotechnology.
Review
Patrick et al., Dunedin, New Zealand. In Archaea, 2014
Finally, we draw attention to recent experiments in which protein engineering was used to modify the activities of the DNA ligase from Pyrococcus furiosus and the RNA ligase from Methanothermobacter thermautotrophicus, thus demonstrating the potential for further work in this area.
A chemical and kinetic perspective on base excision repair of DNA.
Impact
Delaney et al., Providence, United States. In Acc Chem Res, 2014
DNA ligase is responsible for sealing the nick in the backbone and regenerating undamaged duplex.
Aprataxin resolves adenylated RNA-DNA junctions to maintain genome integrity.
Impact
Williams et al., United States. In Nature, 2014
Paradoxically, when DNA ligases encounter nicked DNA structures with abnormal DNA termini, DNA ligase catalytic activity can generate and/or exacerbate DNA damage through abortive ligation that produces chemically adducted, toxic 5'-adenylated (5'-AMP) DNA lesions.
An inhibitor of nonhomologous end-joining abrogates double-strand break repair and impedes cancer progression.
Impact
Raghavan et al., Bengaluru, India. In Cell, 2013
DNA Ligase IV is responsible for sealing of double-strand breaks (DSBs) during nonhomologous end-joining (NHEJ).
Genetic determinants for promoter hypermethylation in the lungs of smokers: a candidate gene-based study.
GeneRIF
Belinsky et al., Albuquerque, United States. In Cancer Res, 2012
Data show that association of study-wide significance (P < 8.2 x 10(-5)) was identified for single-nucleotide polymorphisms (SNP) in TP53, LIG1, and BIK.
Kinetic mechanism of human DNA ligase I reveals magnesium-dependent changes in the rate-limiting step that compromise ligation efficiency.
GeneRIF
O'Brien et al., Ann Arbor, United States. In J Biol Chem, 2011
Kinetic mechanism of human DNA ligase I reveals magnesium-dependent changes in the rate-limiting step that compromise ligation efficiency.
Cellular DNA ligase I is recruited to cytoplasmic vaccinia virus factories and masks the role of the vaccinia ligase in viral DNA replication.
GeneRIF
Moss et al., Bethesda, United States. In Cell Host Microbe, 2010
RNA silencing of human DNA ligase I expression severely reduced replication of viral DNA in cells infected with vaccinia virus ligase-deficient mutants.
CTG/CAG repeat instability is modulated by the levels of human DNA ligase I and its interaction with proliferating cell nuclear antigen: a distinction between replication and slipped-DNA repair.
GeneRIF
Pearson et al., Toronto, Canada. In J Biol Chem, 2009
Data show that disruption of LigI and PCNA interactions influences trinucleotide repeat instability.
DNA ligase I and Nbs1 proteins associate in a complex and colocalize at replication factories.
GeneRIF
Montecucco et al., Pavia, Italy. In Cell Cycle, 2009
Data support the notion that DNA ligase I participates in homology dependent pathways that deal with replication-associated lesions generated when replication fork encounters DNA damage.
More papers using DNA ligase antibodies
CERT-mediated trafficking of ceramide.
Supplier
Shirihai Orian S., In PLoS ONE, 2008
... obtained from Clontech Europe (St Germain-en-Laye, France), and the restriction endonucleases, Taq polymerase and T4 DNA ligase from New England Biolabs (Bioconcept, Allschwil, Switzerland) ...
Targeted delivery of a suicide gene to human colorectal tumors by a conditionally replicating vaccinia virus
Supplier
Black Margaret E. et al., In Cancer gene therapy, 2007
... Restriction endonucleases and T4 DNA ligase were purchased from New England Biolabs (Beverly, MA) ...
DBTSS: database of transcription start sites, progress report 2008.
Supplier
Patil Christopher K., In PLoS ONE, 2007
... T4 DNA polymerase (New England Biolabs, Ipswich, MA) and ligated to 2 µM linkers using T4 DNA ligase (New England Biolabs).
GROMACS 4: Algorithms for Highly Efficient, Load-Balanced, and Scalable Molecular Simulation.
Supplier
Permyakov Eugene A., In PLoS ONE, 2007
... PCR reagents and T4 DNA ligase were purchased from New England Biolabs.
Identification of Culex pipiens complex mosquitoes in a hybrid zone of West Nile Virus transmission in Fresno County, California.
Supplier
Nighorn Alan, In PLoS ONE, 2007
... gel- purified by QIAquick Gel Extraction Kit, and ligated into prepared pET vector by T4 DNA ligase (New England Biolabs).
An expanded set of amino acid analogs for the ribosomal translation of unnatural peptides
Supplier
Ellington Andrew D. et al., In Nucleic Acids Research, 2006
... All restriction enzymes, T4 DNA ligase and vector pBR322 were from New England Biolabs (Ipswich, MA, USA) ...
Molecular basis of MAPK-activated protein kinase 2:p38 assembly.
Supplier
Uversky Vladimir N., In PLoS ONE, 2006
... Restriction enzymes, PCR reagents, and T4 DNA ligase were purchased from New England Biolabs (Beverly, MA) and Invitrogen ...
Clustal W and Clustal X version 2.0.
Supplier
Lee Hwei-Jen et al., In Molecular Vision, 2006
... Restriction enzymes and T4 DNA ligase were purchased from New England Biolabs (Beverly, MA) ...
Rasburicase represents a new tool for hyperuricemia in tumor lysis syndrome and in gout
Supplier
Torres Fernando Araripe Gonçalves et al., In Journal of Biomedicine and Biotechnology, 2006
... T4 DNA ligase was obtained from New England Biolabs.
Serum concentrations of eosinophil cationic protein and eosinophils of patients with Kimura's disease
Supplier
Sideris Diamantis C. et al., In Nucleic Acids Research, 2006
... DNA ligase and T4 polynucleotide kinase were purchased from New England Biolabs.
Evaluation of the immune response induced by multiantigenic DNA vaccine encoding SAG1 and ROP2 of Toxoplasma gondii and the adjuvant properties of murine interleukin-12 plasmid in BALB/c mice
Supplier
Lin Jian-Yin et al., In Parasitology Research, 2006
... I), calf intestine alkaline phosphatase, and T4 DNA Ligase were purchased from New England Biolabs (Beverly MA, USA) ...
Clubbing together on clamps: the key to translesion synthesis
Supplier
Basu Ashis K. et al., In Nucleic Acids Research, 2005
... T4 DNA ligase and T4 polynucleotide kinase were obtained from New England Biolabs (Beverly, MA) ...
CASTp: computed atlas of surface topography of proteins with structural and topographical mapping of functionally annotated residues.
Supplier
oliveira pedro lagerblad, In PLoS ONE, 2005
... RV recognition site of pBluescript II SK (+) (Stratagene) by using of T4 DNA ligase (New England Biolabs, Ipswich, MA) ...
Morphogenetic surfactants and their role in the formation of aerial hyphae in Streptomyces coelicolor
Supplier
van der Donk Wilfred A. et al., In ACS Chemical Biology, 2005
... Restriction endonucleases, DNA polymerases, and T4 DNA ligase were purchased from New England Biolabs or Invitrogen.
Analysis and update of the human aldehyde dehydrogenase (ALDH) gene family
Supplier
Terwilliger Thomas C. et al., In Journal of Structural and Functional Genomics, 2004
... pET-28 vector containing a C-terminal 6-His tag, in frame with the BamHI restriction site using T4 DNA ligase (New England BioLabs), and transformed into BL21(DE3) ...
Neuronal calcium sensor-1 facilitates neuronal exocytosis through phosphatidylinositol 4-kinase
Supplier
Brill Julie A. et al., In The Journal of Cell Biology, 2004
... ) was performed with restriction enzymes and T4 DNA ligase (New England Biolabs, Inc.) ...
WebLogo: a sequence logo generator.
Supplier
Newlon Carol S., In PLoS Genetics, 2003
... site in the pIL07 vector (see below) using T4 DNA Ligase (New England Biolabs).
Altering DNA polymerase incorporation fidelity by distorting the dNTP binding pocket with a bulky carcinogen-damaged template
Supplier
Lloyd R. Stephen et al., In Chemical Research in Toxicology, 2003
... Uracil DNA glycosylase, T4 DNA ligase, and T4 polynucleotide kinase were purchased from New England BioLabs (Beverly, MA) ...
Protein kinase CK2 is coassembled with small conductance Ca(2+)-activated K+ channels and regulates channel gating.
Supplier
Koch Karl-Wilhelm, In PLoS ONE, 2003
... Restriction enzymes, PNGase F, and T4 DNA ligase were from New England Biolabs (Pickering, ON, Canada) ...
Epigenetic programming by maternal behavior
Supplier
Martin Lynn B. et al., In Genetics Research International, 2003
... We then ligated double-stranded EcoRI and Msp/HpaII adaptors to the digested fragments with T4 DNA ligase (New England Biolabs Ipswich, MA, USA) ...
An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography
Supplier
Lin Zhanglin et al., In Microbial Cell Factories, 2002
... I and the T4 DNA ligase were from either New England Biolabs (Beverly, MA) or Takara ...
Mouse MIM, a tissue-specific regulator of cytoskeletal dynamics, interacts with ATP-actin monomers through its C-terminal WH2 domain
Supplier
Lucito Robert et al., In Nucleic Acids Research, 2002
... Enzymes, MspI, McrBC, T4 DNA ligase, were supplied by new England Biolabs.
Immunoglobulin kappa light chain gene rearrangement is impaired in mice deficient for DNA polymerase mu
Supplier
Blanco Luis et al., In Nucleic Acids Research, 2002
... T4 polinucleotide kinase and T4 DNA ligase were from New England Biolabs.
Host factor titration by chromosomal R-loops as a mechanism for runaway plasmid replication in transcription termination-defective mutants of Escherichia coli
Supplier
Sen Ranjan et al., In Journal of Molecular Biology, 2002
... Restriction endonucleases, polynucleotide kinase and T4 DNA ligase were from New England Biolabs.
Genetic identification of a respiratory arsenate reductase.
Supplier
Xu Shuang-yong, In PLoS ONE, 2002
... Restriction enzymes, phosphatase, DNA polymerase mix, and T4 DNA Ligase were obtained from New England Biolabs (Ipswich, MA) ...
Oxidative stress induces nuclear loss of DNA repair proteins Ku70 and Ku80 and apoptosis in pancreatic acinar AR42J cells
Supplier
Turchi John J et al., In BMC Molecular Biology, 2002
... was purchased from Stratagene (La Jolla, CA) and all other restriction enzymes and T4 DNA ligase were purchased from New England Biolabs, Inc ...
The PyMOL Molecular Graphics System.
Supplier
Uversky Vladimir N., In PLoS ONE, 2001
... dNTPs and T4 DNA ligase were obtained from Roche (Germany), and restriction enzymes and T4 polynucleotide kinase from New England BioLabs (Hitchin, UK) ...
Heterologous expression and purification of SpaB involved in subtilin biosynthesis
Supplier
van der Donk Wilfred A. et al., In Biochemistry, 2001
... Restriction endonucleases, DNA polymerases, and T4 DNA ligase were purchased from Invitrogen or New England Biolabs.
Multiple conformational changes in enzyme catalysis
Supplier
Fedorova Olga S. et al., In Nucleic Acids Research, 2001
... Restriction endonuclease XhoI, T4 polynucleotide kinase and T4 DNA ligase were from New England Biolabs (Beverly, MA); restriction endonuclease ...
Coexpression of guanylate kinase with thymidine kinase enhances prodrug cell killing in vitro and suppresses vascular smooth muscle cell proliferation in vivo
Supplier
Black Margaret E. et al., In Cancer gene therapy, 2000
... Restriction endonucleases and T4 DNA ligase were purchased from New England Biolabs (Beverly, MA) ...
A degradation signal located in the C-terminus of p21WAF1/CIP1 is a binding site for the C8 α-subunit of the 20S proteasome
Supplier
Kim Jungho et al., In Biochemical Journal, 2000
... Restriction endonucleases, calf intestinal AP (alkaline phosphatase), the Klenow fragment of DNA polymerase I and T4 DNA ligase were purchased from New England Biolabs.
Fidelity of uracil-initiated base excision DNA repair in DNA polymerase β-proficient and -deficient mouse embryonic fibroblast cell extracts
Supplier
Wilson Samuel H. et al., In Nucleic Acids Research, 2000
... NB1, T4 DNA ligase and all other restriction enzymes were from New England Biolabs (Beverly, MA, USA) ...
Molecular cloning: A laboratory manual.
Supplier
Darlix Jean-Luc, In PLoS ONE, 2000
... T7 RNAP and DNA ligase were purchased from New England Biolabs.
Pyridoxal 5′-phosphate-dependent alpha,beta-elimination reactions: mechanism of O-acetylserine sulfhydrylase
Supplier
Ealick Steven E. et al., In Biochemistry, 2000
... T4 DNA ligase was purchased from New England Biolabs.
An efficient recombination system for chromosome engineering in Escherichia coli.
Supplier
Rutherford Julian, In PLoS ONE, 1999
... 4–20% polyacrylamide gradient Tris-HCl protein gels), IDTDNA (oligonucleotides), New England Biolabs (restriction enzymes, DNA polymerase, DNA ligase), Agrisera (chicken anti-RbcL antibody, Product ...
Robust perfect adaptation in bacterial chemotaxis through integral feedback control
Supplier
Falke Joseph J. et al., In The Journal of General Physiology, 1999
... Kunkel mutagenesis reagents (unmodified T7 DNA polymerase and T4 DNA ligase) as well as restriction enzymes were purchased from New England Biolabs.
HRD gene dependence of ER-associated degradation
Supplier
Hampton Randolph Y. et al., In The Journal of Cell Biology, 1999
... Restriction enzymes, Vent DNA polymerase and T4 DNA ligase were obtained from New England Biolabs.
Cod1p/Spf1p is a P-type ATPase involved in ER function and Ca2+ homeostasis
Supplier
Hampton Randolph Y. et al., In The Journal of Cell Biology, 1999
... Restriction enzymes, Vent DNA polymerase, and T4 DNA ligase were obtained from New England Biolabs, Inc ...
CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family
Supplier
Goud Bruno et al., In The Journal of Cell Biology, 1999
... Culture medium, sodium pyruvate, and glutamine were from GIBCO BRL, restriction enzymes and T4 DNA ligase were from New England Biolabs, Inc., and oligonucleotides were ...
Structural analysis of the catalytic and binding sites of Clostridium botulinum neurotoxin B.
Supplier
Yang Ching-Hong, In PLoS ONE, 1999
... Commercial enzymes (Phusion High-Fidelity DNA Polymerase, BamHI, XhoI, T4 polynucleotide kinase [3′ phosphatase minus], T4 DNA ligase [New England BioLabs, Inc., Bethesda, MD]) were ...
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.
Supplier
Gilbert M. Thomas P., In PLoS ONE, 1999
... ERV1 PCR products- and 5′-dephosphorylated TUPLE1 PCR products were ligated (T4 DNA Ligase in T4 DNA Ligase Buffer, New England Biolabs) and purified ...
A new 34-kilodalton isoform of human fibroblast growth factor 2 is cap dependently synthesized by using a non-AUG start codon and behaves as a survival factor
Supplier
Mills Donald R. et al., In Nucleic Acids Research, 1998
... Restriction endonucleases, oligonucleotide linkers, T4 DNA polymerase and T4 DNA ligase were purchased from New England Biolabs, Inc., Beverly, MA, USA ...
Probing the Saccharomyces cerevisiae centromeric DNA (CEN DNA)-binding factor 3 (CBF3) kinetochore complex by using atomic force microscopy
Supplier
Hwang Deog Su et al., In Nucleic Acids Research, 1998
... and poly(dI–dC), Amersham Biosciences; T4 polynucleotide kinase, New England Biolabs; Pyrobest DNA polymerase and T4 DNA ligase, Takara; QIAEX II gel extraction kit, Qiagen; site-directed mutagenesis kit, Stratagene; ...
Redox-coupled crystal structural changes in bovine heart cytochrome c oxidase
Supplier
Götz Jürgen, In PLoS ONE, 1997
... Restriction enzymes, DNA isolation/purification kits, DNA polymerase, T4 DNA ligase and helper phage were obtained from Amersham (NJ, USA), Invitrogen (CA, USA), or Qiagen (CA, USA) ...
Saccharomyces cerevisiae exonuclease-1 plays a role in UV resistance that is distinct from nucleotide excision repair
Supplier
DasSarma Shiladitya et al., In BMC Genetics, 1997
... Restriction enzymes, calf intestinal phosphatase, T4 DNA polymerase, T4 polynucleotide kinase, and T4 DNA ligase were purchased from New England Biolabs, Beverly, MA ...
Assembly of iron–sulfur clusters. Identification of an iscSUA–hscBA–fdx gene cluster from Azotobacter vinelandii
Supplier
Collins James J et al., In Molecular Systems Biology, 1997
... All plasmids were constructed using restriction endonucleases and T4 DNA ligase from New England Biolabs.
AFLP: a new technique for DNA fingerprinting.
Supplier
Foster John E. et al., In Journal of Insect Science, 1996
... Five µl of ligation mixture containing 0.15 µl of T4 DNA ligase and 0.5 µl of 10X T4 ligase buffer (New England Biolabs), 0.5 µl of 5 pmoles/µl ...
Insertionally inactivated and inducible recA alleles for use in Neisseria.
Supplier
Mayer Claudine, In PLoS ONE, 1996
... Restriction enzymes, Klenow, polynucleotide kinase (PNK), T4 DNA ligase, and M13K07 Helper Phage were purchased from New England Biolabs.
Helicobacter pylori Modulates the T Helper Cell 1/T Helper Cell 2 Balance through Phase-variable Interaction between Lipopolysaccharide and DC-SIGN
Supplier
Appelmelk Ben J. et al., In The Journal of Experimental Medicine, 1996
... addition of 50 pmol each of the EcoRI adaptor and MseI adaptor, 1.2 U of T4 DNA ligase (BD Biosciences), 1 mM ATP, and ligase ...
Multiple interactions control the intracellular localization of the herpes simplex virus type 1 capsid proteins
Supplier
Geraghty Robert J., In PLoS ONE, 1995
... All enzymes used for cloning procedures were purchased from Takara (Dalian, China) except for T4 DNA ligase from New England Biolabs (MA, USA) ...
Allelic exchange in Mycobacterium tuberculosis with long linear recombination substrates
Supplier
Kobayashi Motomasa et al., In Marine Drugs, 1995
... DNA restriction enzymes and T4 DNA ligase were obtained from New England BioLabs INC ...
Concentrative sorting of secretory cargo proteins into COPII-coated vesicles
Supplier
Schekman Randy et al., In The Journal of Cell Biology, 1994
... cutting pPL257 with SpeI and SacII, treating with mung bean nuclease, and ligating with T4 DNA Ligase (New England Biolabs, Inc.) ...
Host cell proteins binding to domain IV of the 5′ noncoding region of poliovirus RNA.
Supplier
Racaniello Vincent, In PLoS Pathogens, 1994
... the linear vector was gel-purified, phenol-chloroform extracted and ethanol precipitated, and then re-ligated with T4 DNA ligase (New England BioLabs) to re-circularize the plasmid ...
CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
Supplier
Matthews Rowena G., In PLoS Biology, 1993
... Restriction enzymes and T4 DNA ligase were from New England Biolabs (Beverly, Massachusetts, United States) ...
Phase variation of lipopolysaccharide directs interconversion of invasive and immuno-resistant phenotypes of Neisseria gonorrhoeae
Supplier
Stein Daniel C. et al., In The Journal of Experimental Medicine, 1992
... Restriction enzymes, DNA polymerases, and T4 DNA ligase were obtained from New England Biolabs.
Cotranslational amino-terminal processing of cytosolic poteins
Supplier
Jones Dafydd, In PLoS ONE, 1987
... T4 DNA ligase, restriction endonucleases and PCR reagents, were purchased from New England Biolabs (Tokyo, Japan) ...
Two RNA species co-purify with RNase P from the fission yeast Schizosaccharomyces pombe.
Supplier
Bahn Yong-Sun, In PLoS ONE, 1985
... Restriction endonucleases, DNA polymerase I Klenow fragment, T4 polynucleotide kinase, and T4 DNA ligase were purchased from New England Biolabs (Cambridge, MA) ...
Continuous association of Escherichia coli single-stranded DNA binding protein with stable complexes of RecA protein and single-stranded DNA
Supplier
Haber James E., In PLoS Biology, 1985
... T4 DNA polymerase was purchased from New England Biolabs, and T4 DNA ligase was purchased from Promega (Madison, Wisconsin, United States) and New England Biolabs.
Sealing of gaps in duplex DNA by T4 DNA ligase
Supplier
Mrksich Milan et al., In Nucleic Acids Research, 1981
... and 1% Trition X-100; pH 7.6 at 25°C) and T4 DNA ligase provided with 10× reaction buffer (500 mM Tris–HCl, 100 mM MgCl2, 100 mM DTT and 10 mM ATP; pH 7.5 at 25°C) were used as received from New England BioLabs Inc ...
Deletion mutations of the immunity region of coliphage lambda.
Supplier
Semsey Szabolcs, In PLoS ONE, 1974
... Restriction enzymes and T4 DNA ligase were from New England Biolabs.
Errors in DNA replication as a basis of malignant changes
Supplier
Sweasy Joann B. et al., In Nucleic Acids Research, 1973
... (UDG) (M0280S), human AP endonuclease I (APE1) (M0282S), terminal transferase (M0252S), T4 PNK (M0201S)] and T4 DNA ligase (M0202S) were purchased from New England Biolabs.
The initial step in leucine biosynthesis
Supplier
Palittapongarnpim Prasit et al., In BMC Microbiology, 1960
... Restriction enzymes and T4 DNA ligase were obtained from New England Biolabs, Bevery, MA, USA ...
N-Succinyl-L-diaminopimelic acid
Supplier
Creus Marc et al., In Bioinorganic Chemistry and Applications, 1958
... T4-DNA ligase and HindIII from New England Biolabs.
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