Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.
Beijing, China. In Proc Natl Acad Sci U S A, Feb 2016
By using a clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system, we generated mouse CH12F3 cell lines in which, in addition to Lig4, either Lig1 or nuclear Lig3, representing the cells containing a single DNA ligase (Lig3 or Lig1, respectively) in their nucleus, was completely ablated.
Why Johnny can't clone: Common pitfalls and not so common solutions.
Atlanta, United States. In Biotechniques, Sep 2015
In particular, the virtues of agarose gel electrophoresis monitoring, the nature of the interactions between DNA and silica, and challenges associated with thermostable DNA polymerases, restriction endonucleases, and T4 DNA ligase are explored.
Archaeal Nucleic Acid Ligases and Their Potential in Biotechnology.
More papers using
Dunedin, New Zealand. In Archaea, 2014
Finally, we draw attention to recent experiments in which protein engineering was used to modify the activities of the DNA ligase from Pyrococcus furiosus and the RNA ligase from Methanothermobacter thermautotrophicus, thus demonstrating the potential for further work in this area.