In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro.
In PLoS ONE, 2006
... CA), actin, phospho-Akt, Akt and PTEN antibodies (Santa Cruz, CA), Caspase-9 antibody (Millipore, Billerica, MA), DJ-1 antibody (MBL International, Woburn, MA), HRP ...
Doxorubicin induces apoptosis in Jurkat cells by mitochondria-dependent and mitochondria-independent mechanisms under normoxic and hypoxic conditions.
Medellín, Colombia. In Anticancer Drugs, 02 Apr 2015
In addition, dxr (10 μmol/l) induced activation and/or nuclei translocation of NF-κB (6.6, 1.6-fold increase), p53 (4.3, 3.1 f), c-Jun (9.5, 5.0 f), apoptosis-inducing factor (AIF) (1.9, 3.9 f), caspase-3 (3.7, 1.9 f), overexpression of Parkin (2.1, 1.2 f)/PINK-1 (2.1 f) proteins, and reduced DJ-1 levels by half compared with untreated cells under normoxia, according to immunofluorescence and in-cell western analysis, respectively.
Mitochondria in the aetiology and pathogenesis of Parkinson's disease.
London, United Kingdom. In Lancet Neurol, 2008
The proteins that are associated with familial PD--PTEN-induced putative kinase 1 (PINK1), DJ-1, alpha-synuclein, leucine-rich repeat kinase 2, and, possibly, parkin--are either mitochondrial proteins or are associated with mitochondria, and all interface with the pathways of oxidative stress and free radical damage.
London, United Kingdom. In Lancet, 2006
Recently identified nuclear gene mutations of mitochondrial proteins include mutations of frataxin causing Friedreich's ataxia, PINK1, DJ1 causing Parkinson's disease and POLG causing infantile mtDNA depletion syndrome, ophthalmoplegia, parkinsonism, male subfertility and, in a transgenic mouse model, premature senescence.