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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Aug 2016.

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DAPI, GKAP
binds the guanylate kinase-like domain of the PSD-95 family of synaptic ion channel clustering proteins; may play a role in protein-protein interactions at the synaptic junction [RGD, Feb 2006] (from NCBI)
Top mentioned proteins: PrP, CAN, caspase-3, V1a, bcl-2
Papers using DAPI antibodies
Chronic ethanol consumption in mice alters hepatocyte lipid droplet properties
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Casey Carol A. et al., In International Journal of Hepatology, 2010
... UltraCruz mounting media containing 4,6-diamidino-2-phenylindole (DAPI) was obtained from Santa Cruz Biotechnology, Inc (Santa Cruz, CA) ...
Update on Aurora kinase targeted therapeutics in oncology
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Ulisse Salvatore et al., In BMC Cancer, 2010
... Mounting Medium with DAPI was from Vector Laboratories (Burlingame, KS) ...
Generation of a non-leaky heat shock-inducible Cre line for conditional Cre/Lox strategies in zebrafish
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Brand Michael et al., In Neural Development, 2010
... GFP and DAPI staining in heat-shocked non-transgenic animals ...
Divergent Sp1 protein levels may underlie differential expression of UDP-glucose dehydrogenase by fibroblasts: Role in susceptibility to orbital Graves disease.
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Ludgate Marian, In PLoS ONE, 2010
... Goat anti-rabbit secondary Ab conjugated with FITC and mounting medium containing propidium iodide (PI) or DAPI were obtained from VECTOR Laboratories (Burlingame, CA) ...
Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo
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Hong Yunhan et al., In International Journal of Biological Sciences, 2010
... Nuclear staining was done by using 4'-6-Diamidino-2-phenylindole (DAPI) in the Gold Antifade reagent (Invitrogen, CA).Microscopy and Photography.
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Papers on DAPI
MicroRNA-509-3p increases the sensitivity of epithelial ovarian cancer cells to cisplatin-induced apoptosis.
New
Jiang et al., Guangzhou, China. In Pharmacogenomics, Feb 2016
MATERIALS & METHODS: The regulation of XIAP by miR-509-3p was investigated by luciferase reporter assay, real-time PCR and immunobloting, and the roles of miR-509-3p in cellular proliferation and apoptosis were accessed through MTT and DAPI assays.
Decellularization of porcine skeletal muscle extracellular matrix for the formulation of a matrix hydrogel: a preliminary study.
New
Lv et al., Chengdu, China. In J Cell Mol Med, Feb 2016
The resulting ECM was evaluated using haematoxylin and eosin, 4-6-diamidino-2-phenylindole (DAPI) staining, and DNA quantification.
MicroRNA-155 promotes apoptosis in SKOV3, A2780, and primary cultured ovarian cancer cells.
New
He et al., Guangzhou, China. In Tumour Biol, Feb 2016
4',6-Diamidino-2-phenylindole (DAPI) staining and terminal-deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) techniques were used to determine the effects of a miR-155 mimic or inhibitor on the apoptotic ratio of ovarian cancer cells induced by cisplatin.
Multiparametric luminescent cell viability assay in toxicology models: A critical evaluation.
New
Kőszegi et al., Pécs, Hungary. In J Pharmacol Toxicol Methods, Feb 2016
Estimation of cell number was done by DAPI fluorescence.
Antimicrobial Activity of ILTI, a Kunitz-Type Trypsin Inhibitor from Inga laurina (SW.) Willd.
New
Maria-Neto et al., Campo Grande, Brazil. In Curr Microbiol, Feb 2016
Finally, ILTI was coupled with fluorescein isothiocyanate, and subsequent treatment of C. tropicalis and C. buinensis with DAPI revealed the presence of the labeled protein in the intracellular spaces.
Soft matrices inhibit cell proliferation and inactivate the fibrotic phenotype of deep endometriotic stromal cells in vitro†.
New
Darcha et al., Clermont-Ferrand, France. In Hum Reprod, Feb 2016
The cellular proliferation index (CPI), assessed as the percentage of Ki67-positive cells among the total number of nuclei stained by 4',6-diamidino-2-phenylindole (DAPI) was determined.
Silvestrol induces early autophagy and apoptosis in human melanoma cells.
New
Burdette et al., Chicago, United States. In Bmc Cancer, Dec 2015
In silvestrol-treated cells, DAPI staining of nuclear chromatin displayed nucleosomal fragments.
Commonly used mesenchymal stem cell markers and tracking labels: Limitations and challenges.
Review
Lue et al., San Francisco, United States. In Histol Histopathol, 2013
The cell membrane label DiI can be adsorbed by host cells, and nuclear stains Hoechst dyes and DAPI can be transferred to host cells.
Theranostic nanoshells: from probe design to imaging and treatment of cancer.
Review
Impact
Halas et al., Houston, United States. In Acc Chem Res, 2011
Double-stranded DNA nanoshells also provide a way to deliver small molecules into cells: we describe the delivery and light-triggered release of DAPI (4',6-diamidino-2-phenylindole), a dye molecule used to stain DNA in the nuclei of cells.
Dlg1 binds GKAP to control dynein association with microtubules, centrosome positioning, and cell polarity.
GeneRIF
Etienne-Manneville et al., Paris, France. In J Cell Biol, 2010
The GKAP controls the forces operating on microtubules and play a fundamental role in centrosome positioning and cell polarity.
Degradation of postsynaptic scaffold GKAP and regulation of dendritic spine morphology by the TRIM3 ubiquitin ligase in rat hippocampal neurons.
GeneRIF
Sheng et al., Cambridge, United States. In Plos One, 2009
postsynaptic scaffold GKAP is degraded and dendritic spine morphology is regulated by the TRIM3 ubiquitin ligase in rat hippocampal neurons
XIST RNA and architecture of the inactive X chromosome: implications for the repeat genome.
Review
Lawrence et al., Worcester, United States. In Cold Spring Harb Symp Quant Biol, 2009
For example, evidence indicates that XIST RNA acts at the architectural level of the whole chromosome to induce formation of a silent core enriched for nongenic and repetitive (Cot-1) DNA, which corresponds to the DAPI-dense Barr body.
Ros-induced histone modifications and their role in cell survival and cell death.
Review
Lau et al., Tucson, United States. In Drug Metab Rev, 2005
However, H3 phosphorylation occurred concomitant with overall chromatin condensation, as revealed by decreased sensitivity of chromatin to digestion by micrococcal nuclease and by DAPI staining of nuclei.
Determination of absolute protein numbers in single synapses by a GFP-based calibration technique.
Impact
Okabe et al., Tokyo, Japan. In Nat Methods, 2005
At maturity we calculated that a single excitatory postsynaptic site contains 100-450 of individual postsynaptic proteins, such as PSD-95, GKAP, Shank and Homer.
DNA minor groove binders as potential antitumor and antimicrobial agents.
Review
Romagnoli et al., Ferrara, Italy. In Med Res Rev, 2004
Diarylamidines such as DAPI, berenil, and pentamidine; bis-benzimidazoles such as Hoechst 33258; ecteinascidins, pyrrololo [2,1-c]-[1,4]-benzodiazepines (PBDs), CC-1065, and distamycins are the classes discussed in this review article.
Linker histones are not essential and affect chromatin condensation in vivo.
Impact
Gorovsky et al., Rochester, United States. In Cell, 1995
Histone H1 knockout (delta H1) cells have enlarged DAPI-stained macronuclei and normal-sized micronuclei, while MicLH knockout (delta MicLH) cells have enlarged micronuclei and normal-sized macronuclei.
The NDA3 gene of fission yeast encodes beta-tubulin: a cold-sensitive nda3 mutation reversibly blocks spindle formation and chromosome movement in mitosis.
Impact
Yanagida et al., In Cell, 1984
DAPI staining and indirect immunofluorescence microscopy showed three condensed chromosomes but no spindle.
More papers using DAPI antibodies
Persistent eNOS activation secondary to caveolin-1 deficiency induces pulmonary hypertension in mice and humans through PKG nitration
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Malik Asrar B. et al., In The Journal of Cell Biology, 2008
... Cy-5-conjugated donkey anti–mouse, anti–goat, and anti–rabbit (Jackson ImmunoResearch Laboratories, Inc.), Alexa Fluor 532 phalloidin, and DAPI (Sigma-Aldrich).We used protein A/G beads (Santa Cruz Biotechnology, Inc.), DHE, and Dynabeads ...
A role for the juxtamembrane cytoplasm in the molecular dynamics of focal adhesions.
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Parsons Maddy, In PLoS ONE, 2008
... Rhodamine-conjugated phalloidin (Invitrogen, Carlsbad, CA) and 4′,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) were used for fluorescent ...
Targeting neural precursors in the adult brain rescues injured dopamine neurons.
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Sham Mai Har, In PLoS ONE, 2008
... For immunofluorescence, a DAPI (4, 6-diamidino-2-phenylindole, Sigma Aldrich, St Louis, MO, USA, ...
Elevation of human α-defensins and S100 calcium binding protein A8 and A9 in tear fluid of pterygium patients.
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Tong Louis et al., In Molecular Vision, 2008
... Goat anti-mouse HRP-conjugated antibody, radio immunoprecipitation (RIPA) lysis buffer, and UltraCruz Mounting Medium containing 4.6-diamidino-2-phenylindole (DAPI) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) ...
Regulation of progenitor cell proliferation and granulocyte function by microRNA-223
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Wood Matthew JA et al., In Molecular Therapy. Nucleic Acids, 2007
... Slides were mounted with Vectashield Hard Set mounting medium (with DAPI) (Vector Laboratories, Peterborough, UK) ...
Age-related auditory pathology in the CBA/J mouse.
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Allodi Silvana, In PLoS ONE, 2007
... with the sections or the whole mounts were coverslipped using an antifade mountant containing 4′,6-diamidino-2-phenylindole (DAPI) to label nuclei (Vectashield; Vector Laboratories).
Criteria for annotation of plant microRNAs.
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Newbigin Edward, In PLoS ONE, 2007
... The suspensions were stained with 2 µg/ml DAPI (4′,6-diamino-2-phenylindole) and 33,000 and 19,000 copies of telocentric chromosomes 5AS and 5AL, respectively, were sorted using a FACSVantage SE flow cytometer (Becton Dickinson, San José, USA) into ...
Course of preeclampic glomerular injury after delivery
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Sass N. et al., In Nephron Extra, 2007
... Then Vectashield (mounting medium for fluorescence) with DAPI (4′6-diamino-2-phenylindole dihydrochloride) was applied (H-1200 Vector Laboratories, Inc., USA).
Loss of desmoglein 1 expression associated with worse prognosis in head and neck squamous cell carcinoma patients
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Wahl James K. et al., In Dermatology Research and Practice, 2007
... were incubated with appropriate FITC-conjugated antimouse secondary antibodies and mounted in vectashield mounting media containing DAPI (Vector Laboratories, Burlingame, CA) ...
Dendritic-cell immunotherapy: from ex vivo loading to in vivo targeting
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Ito Yoshihiro et al., In International Journal of Nanomedicine, 2006
... 4′,6-Diamidino-2-phenylindole (DAPI) and Vectashield mounting medium were purchased from Vector Laboratories (Burlingame, CA) ...
Gepard: A rapid and sensitive tool for creating dotplots on genome scale.
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Salamin Nicolas, In PLoS ONE, 2006
... Chromosomes in suspension were stained by 2 µg/ml DAPI (4′,6-diamidino-2-phenylindole) and analysed using a FACSVantage SE flow cytometer (Becton Dickinson, San José, USA) at ...
Tumor microvasculature and microenvironment: targets for anti-angiogenesis and normalization
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Kumar GS Vinod et al., In International Journal of Nanomedicine, 2006
... F-68, rhodamine, 5-FU, MTT, Dulbecco’s modified Eagle’s medium (DMEM), trypsin-ethylenediaminetetraacetic acid (EDTA) and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (Steinheim, Germany), and fetal bovine serum (FBS) from Gibco (Life Technologies AG, Basel, Switzerland) ...
Autogenous and nonautogenous control of response in a genetic network.
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Qiu Jianming, In PLoS ONE, 2005
... Dead cells were excluded using DAPI (Roche, Indianapolis, IN) or 7-AAD (BD Biosciences) viability dye ...
Oncogenic PI3K and its role in cancer
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Nie Daotai, In PLoS ONE, 2005
... Louis, MO) Propedium Iodide and Ultracruz DAPI mounting medium was obtained from Santa Cruz Biotechnology Inc.(Santa Cruz, CA), Z-VAD ...
Reduction of insulin-stimulated glucose uptake in L6 myotubes by the protein kinase inhibitor SB203580 is independent of p38MAPK activity
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Blough E. R. et al., In Experimental Diabetes Research, 2004
Antirabbit and antimouse fluorescein antibodies and VectaSheild Hardset Mounting Medium with DAPI was purchased from Vector Laboratories (Burlingame, Calif, USA).
Studies on PEGylated and drug-loaded PAMAM dendrimers
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Li Qi et al., In International Journal of Nanomedicine, 2004
... and BALB/c female athymic nude mice from the Shanghai Cancer Institute (Shanghai, China); 4’, 6-diamidino-2-phenylindole (DAPI) from Shanghai Future Biological Technology Co, Ltd (Shanghai, China); rhodamine B (Rb) from Sigma-Aldrich (St Louis, MO); and the WST-8 Cell Proliferation Assay Kit from Cayman Chemical (Ann Arbor, MI) ...
Quantitative fluorescence microscopy to probe intracellular microenvironments.
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Kurtti Timothy J. et al., In Journal of Insect Science, 2004
... HkFITC-Bb were counted by diluting 1 µl bacterial stock into 4 µl VectaShield mounting medium containing 4′-6-Diamidino-2-phenylindole (DAPI-VectaShield; Vector Laboratories, ...
Regulation of ADAM12 cell-surface expression by protein kinase C epsilon.
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Huard Johnny, In PLoS ONE, 2003
... Vectashield mounting medium with 4′-6-Diamidino-2-phenylindole (DAPI) was purchased from Vector Laboratories (Burlingame, CA, ...
Pegylated liposomeal doxorybicin: proof of principle using preclinical animal models and pharmacokinetic studies
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Cox Karen et al., In Drug Target Insights, 2003
... The nuclei stain fluorescent dye DAPI (4’, 6-diamidino-2-phenylindole) containing mount medium was a gift from Dako Inc ...
Death by design: apoptosis, necrosis and autophagy
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Herz Werner et al., In Marine Drugs, 2003
... Vectashield, Vectashield containing DAPI (0.5 μg/ml) were from Vector Laboratories (UK) ...
Inhibition of formyl-methionyl-leucyl-phenylalanine-stimulated respiratory burst in human neutrophils by adrenaline: inhibition of Phospholipase A2 activity but not p47phox phosphorylation and translocation
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Zhang Jing et al., In Journal of Neuroinflammation, 2003
... 4,6-Diamidino-2-phenylindole (DAPI)-containing mounting medium was from Vector Laboratories (Burlingame, CA) ...
Interleukin-8 expression is increased in senescent prostatic epithelial cells and promotes the development of benign prostatic hyperplasia.
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Lobaccaro Jean-Marc A., In PLoS ONE, 2003
... 4’,6-diamidino-2-phenylindole (DAPI) was purchased from Vector Laboratories (Burlingame, CA) ...
Multigene reverse transcription-PCR profiling of circulating tumor cells in hormone-refractory prostate cancer
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Burke Julian F et al., In Breast Cancer Research : BCR, 2003
... The 4'6-diamidino-2-phenylindole (DAPI) mounting medium (Vector Laboratories, Burlingame, CA, USA) was ...
A physiologic role for testosterone in limiting estrogenic stimulation of the breast
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Bondy Carolyn et al., In Breast Cancer Research, 2002
... The slides were stained with 4',6-diamidino-2-phenylindole (DAPI) and examined using a Leica epifluorescence microscope; fluorescence images ...
Centrosome reorientation in wound-edge cells is cell type specific
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Etienne-Manneville Sandrine et al., In The Journal of Cell Biology, 2001
... Scientific; monoclonal anti-Dlg1 and anti-DHC from Santa Cruz Biotechnology, Inc.; anti-pericentrin and anti-DIC from Covance; anti-GKAP from Covalab; and anti-GFP from Roche ...
Signal analysis of behavioral and molecular cycles.
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Preiss Thomas, In PLoS ONE, 2001
... Small aliquots of the lysate were removed after each sonication pulse, stained with DAPI (Vector laboratories H-1200) and examined by ...
Analysis of chromosomal and organellar DNA of somatic hybrids between Triticum aestivum and Haynaldia villosa Schur
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Łapiński Mirosław et al., In Journal of Applied Genetics, 2000
... FISH, the preparations were mounted and counterstained in Vectashield (Vector Laboratories) containing 2.5 μg/ml of DAPI (Sigma) or 1.5 μg/ml of propidium iodide (Vector Laboratories); for GISH - the preparations ...
The machinery of programmed cell death
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Cai Xiaohui et al., In International Journal of Nanomedicine, 2000
... 4, 6-diamidino-2-phenylindole (DAPI) was obtained from Santa Cruz Biotechnology Inc (Santa Cruz, CA) ...
Rapid detection of west nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay.
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Banfield Bruce W., In PLoS ONE, 1999
... cocktail, FuGENE 6 transfection regent from Roche Diagnostics (Laval, Quebec); ProLong® Gold Antifade reagent with 4′-6-diamidino-2-phenylindole (DAPI), media and fetal bovine serum (FBS) for cell culture from Invitrogen; Pierce BCA Protein Assay Kit from Thermo Scientific (Rockford, IL); High Capacity ...
Phylogeny of phyllostomid bats (Mammalia, Chiroptera): Data from diverse morphological systems, sex chromosomes and restriction sites
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de Souza Maria José et al., In Genetics and Molecular Biology, 1999
... /DA/DAPI.Photomicrographs were taken by means of a Leica DMLB photomicroscope for conventional, ...
MT1-MMP–dependent neovessel formation within the confines of the three-dimensional extracellular matrix
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Weiss Stephen J. et al., In The Journal of Cell Biology, 1999
... Alexa Fluor 488 anti–rat IgG secondary antibody (Molecular Probes), and the nuclei were visualized with DAPI (Vector Laboratories).
Trans-fatty acid content in hydrogenated oils and estimated intake
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Fujimoto Yohko et al., In Journal of Clinical Biochemistry and Nutrition, 1998
... 2',7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) was purchased from Molecular Probes (Eugene, OR) and 4',6-diamino-2-phenylindole (DAPI) was from Merck (San Diego, CA) ...
A mechanism of repression of TGFb/Smad signaling by oncogenic Ras.
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Chegini Nasser et al., In Reproductive biology and endocrinology : RB&E, 1998
... After incubation, the cells were washed with PBS and Vectashield with DAPI (Vector Laboratories) as the mounting medium ...
Activation of nuclear factor-κB is necessary for myotrophin-induced cardiac hypertrophy
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Sen Subha et al., In The Journal of Cell Biology, 1997
... Vectashield with DAPI was purchased from Vector Laboratories.
Mammographic densities and breast cancer risk
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dos-Santos-Pinto-Junior Décio et al., In Medicina Oral, Patología Oral y Cirugía Bucal, 1997
... After washing five times with 1X PBS, the coverslips were mounted with mounting medium (Vectashield: DAPI, Vector Laboratories, Ind., Burlingame, CA, USA) ...
Rhamm−/− fibroblasts are defective in CD44-mediated ERK1,2 motogenic signaling, leading to defective skin wound repair
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Turley Eva A. et al., In The Journal of Cell Biology, 1997
... 10 μM U0126 (BD Biosciences); collagen (Vitrogen100; Cohesion); Matrigel (BD Biosciences); immunofluorescence mounting medium with DAPI (Vectashield; Vector Laboratories); Cytoseal (Richard-Allan Scientific); and ...
Leptomycin B is an inhibitor of nuclear exportinhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNA
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Paschal Bryce M. et al., In The Journal of Cell Biology, 1996
... Cells from both standard and two-step export reactions were fixed with formaldehyde, stained with DAPI, and mounted using Vectashield medium (Vector Laboratories).
Pharmacology of oleanolic acid and ursolic acid.
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Bauer Joseph Alan, In PLoS ONE, 1994
... 4′,6-diamidino-2-phenylindole (DAPI) and PE-labeled anti-MRP1 (QCRL-2) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA) ...
Methods for clonal growth and serial cultivation of normal human epidermal keratinocytes and mesothelial cells.
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Karl Mike O., In PLoS ONE, 1988
... DAPI reagent (Sigma-Aldrich, Inc.) and mounted in Mowiol (Sigma-Aldrich, Inc.) or with the Vectashield + DAPI (Vector Laboratories).
Temperature-dependent physiological stability of rat skeletal muscle in vitro.
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Vina Jose, In PLoS ONE, 1984
... VECTASHIELD HardSet Mounting Medium with DAPI was from Vector Laboratories (Burlingame, CA) ...
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