Experimental TLR4 inhibition improves intestinal microcirculation in endotoxemic rats.
Halifax, Canada. In Microvasc Res, 30 Sep 2015
MATERIALS AND METHODS: The intestinal microcirculation was studied by intravital microscopy in four groups of Lewis rats (n=10 per group): healthy controls (CON group), endotoxemic animals (15mg/kg lipopolysaccharide, LPS group), endotoxemic animals treated with a TLR4 antagonist (1mg/kg CRX-526, LPS+CRX526 group), and controls treated with CRX-526 (C-CRX526 group).
Characterization of three-dimensional retinal tissue derived from human embryonic stem cells in adherent monolayer cultures.
Pittsburgh, United States. In Stem Cells Dev, 18 Sep 2015
We found that deriving such tissue in adherent conditions robustly induces all eye field genes (RX, PAX6, LHX2, SIX3, SIX6) and produces four layers of pure populations of retinal cells: RPE (expressing NHERF1, EZRIN, RPE65, DCT, TYR, TYRP, MITF, PMEL), early photoreceptors (coexpressing CRX and RCVRN), inner nuclear layer neurons (expressing CALB2) and retinal ganglion cells (RGCs, expressing BRN3B and Neurofilament [NF] 200).