Inhibition of RPE65 Retinol Isomerase Activity by Inhibitors of Lipid Metabolism.
United States. In J Biol Chem, Jan 2016
To investigate potential interactions between RPE65 and lipid metabolism enzymes, HEK293-F cells were transfected with expression vectors for visual cycle proteins and co-transfected with either fatty acyl:CoA ligases (ACSLs) 1, 3 or 6, or the SLC27A family fatty acyl-CoA synthase FATP2/SLCA27A2 to test their effect on isomerase activity.
A novel MVA-mediated pathway for isoprene production in engineered E. coli.
Qingdao, China. In Bmc Biotechnol, Dec 2015
A novel biosynthetic pathway of isoprene in E. coli was established by co-expressing the heterologous mvaE gene encoding acetyl-CoA acetyltransferase/HMG-CoA reductase and mvaS gene encoding HMG-CoA synthase from Enterococcus faecalis, fatty acid decarboxylase (OleTJE) and oleate hydratase (OhyAEM).
Carbon monoxide. Toxic gas and fuel for anaerobes and aerobes: carbon monoxide dehydrogenases.
Berlin, Germany. In Met Ions Life Sci, 2013
Use of CO as metabolic fuel for microbes relies on enzymes like carbon monoxide dehydrogenase (CODH) and acetyl-CoA synthase (ACS), which catalyze conversions resembling processes that eventually initiated the dawn of life.CODHs catalyze the (reversible) oxidation of CO with water to CO2 and come in two different flavors with unprecedented active site architectures.
Enzymes with molecular tunnels.
College Station, United States. In Acc Chem Res, 2003
Since the first molecular tunnel within tryptophan synthase was discovered in 1988, tunnels within carbamoyl phosphate synthetase, glutamine phosphoribosylpyrophosphate amidotransferase, asparagine synthetase, glutamate synthase, imidazole glycerol phosphate synthase, glucosamine 6-phosphate synthase, and carbon monoxide dehydrogenase/acetyl-CoA synthase have been identified.