Transposon Mutagenesis Identifies Novel Genes Associated with Staphylococcus aureus Persister Formation.
Shanghai, China. In Front Microbiol, 2014
We found 13 insertion mutants with significantly lower persister numbers under several stress conditions, including sdhA, sdhB, ureG, mnhG1, fbaA, ctaB, clpX, parE, HOU_0223, HOU_0587, HOU_2091, HOU_2315, and HOU_2346, which mapped into pathways of oxidative phosphorylation, TCA cycle, glycolysis, cell cycle, and ABC transporters, suggesting that these genes and pathways may play an important role in persister formation and survival.
Structural and functional analysis of proteins by high-speed atomic force microscopy.
Kyoto, Japan. In Adv Protein Chem Struct Biol, 2011
In this chapter, we describe the HS-AFM analysis of the dynamic molecular processes in photoactivated bacteriorhodopsin, membrane-mediated protein-protein interactions, ATP-induced conformational changes in purinergic receptors, the two-dimensional crystal structure of streptavidin, the nature of FtsZ polymers, the role of ClpX in the regulation of FtsZ polymer dynamics, the function of restriction enzymes, the action of motor proteins, the movement of TrCel7A on crystalline cellulose substrates, and the antimicrobial peptide activity on individual bacterial cells.