RND-type drug eﬄux pumps from Gram-negative bacteria: molecular mechanism and inhibition.
Adelaide, Australia. In Front Microbiol, 2014
Recent successes on the structure determination and functional analysis of the AcrB and MexB components of the AcrAB-TolC and MexAB-OprM drug eﬄux systems as well as the structure of the fully assembled, functional triparted AcrAB-TolC complex significantly contributed to our understanding of the mechanism of substrate transport and the options for inhibition of eﬄux.
Structure of the AcrAB-TolC multidrug efflux pump.
Houston, United States. In Nature, 2014
This pump assembly comprises the outer-membrane channel TolC, the secondary transporter AcrB located in the inner membrane, and the periplasmic AcrA, which bridges these two integral membrane proteins.
Efflux pump-mediated antibiotics resistance: insights from computational structural biology.
Bonn, Germany. In Interdiscip Sci, 2014
Here we review the employment of molecular dynamics computer simulations to investigate RND efflux pumps focusing on our group's recent contributions to this field studying questions of energy conversion and substrate transport in the inner membrane antiporter AcrB in Escherichia coli as well as access regulation and gating mechanism in the outer membrane efflux ducts TolC and OprM in E. coli and Pseudomonas aeruginosa.
Broad-specificity efflux pumps and their role in multidrug resistance of Gram-negative bacteria.
Berkeley, United States. In Fems Microbiol Rev, 2012
The recent investigation on the efflux pump AcrB at its structural and physiological levels, including the identification of drug affinity sites and kinetic parameters for various antibiotics, may pave the way towards the rational development of an improved new generation of antibacterial agents as well as efflux inhibitors in order to efficiently combat efflux-based resistance mechanisms.
Mutation analysis of CHRNA1, CHRNB1, CHRND, and RAPSN genes in multiple pterygium syndrome/fetal akinesia patients.
Birmingham, United Kingdom. In Am J Hum Genet, 2008
No CHRNA1, CHRNB1, or CHRND mutations were detected, but a homozygous RAPSN frameshift mutation, c.1177-1178delAA, was identified in a family with three children affected with lethal fetal akinesia sequence.