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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Aug 2016.

Checkpoint kinase 1

Chk1, checkpoint kinase, checkpoint kinase 1
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011] (from NCBI)
Top mentioned proteins: Chk2, p53, Atm, CAN, PCNA
Papers using Chk1 antibodies
Sphingosine kinase 1 and 2 regulate the capacity of mesangial cells to resist apoptotic stimuli in an opposing manner.
Supplier
Ahmad Aamir, In PLoS ONE, 2007
... Antibodies against Beclin 1 (Atg 6) and Chk1, D-erythro-sphingosine and the PKC inhibitor Ro 31-8220 were obtained from Santa Cruz Biotechnology (supplied by LabForce AG, ...
Initiation of high frequency multi-drug resistance following kinase targeting by siRNAs.
Supplier
Cotterill Sue, In PLoS ONE, 2006
... (Upstate), anti-GAPDH (Abcam), anti-ß-actin (Abcam), Chk1 G-4 sc8408 (Santa Cruz), P-S296 Chk1 2349 S (Cell Signaling), P-S780 Rb 1182-1 (Epitomics) ...
Benzyl isothiocyanate-induced DNA damage causes G2/M cell cycle arrest and apoptosis in human pancreatic cancer cells
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Srivastava S K et al., In British Journal of Cancer, 2005
... Chk2, Cdc25C, Cdk1, CyclinB1, cleaved fragments of caspase-3, poly(ADP-ribose) polymerase (PARP) and human-specific Signal Silence Chk1-SiRNA kit were procured from Cell Signaling Technology Inc ...
The comet assay: a method to measure DNA damage in individual cells
Supplier
Fritz G et al., In Cell Death & Disease, 2005
... antibody (Abcam, Cambridge, MA, USA), p-Chk1 (Ser 345), Chk1 (Cell Signaling Technology, Inc., Beverly, MA, ...
Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair.
Supplier
Kirkpatrick David T., In PLoS ONE, 2004
... (Thermo Scientific), anti-CHK1 (Santa Cruz), anti-CHK2 (Santa Cruz), anti-phospho-CHK1 (Ser 345) (Cell signaling), anti-phospho-CHK2 (Thr 68) (Cell ...
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Papers on Chk1
Cancer-Specific Synthetic Lethality between ATR and CHK1 Kinase Activities.
New
Helleday et al., Stockholm, Sweden. In Cell Rep, Feb 2016
ATR and CHK1 maintain cancer cell survival under replication stress and inhibitors of both kinases are currently undergoing clinical trials.
Overcoming Pluripotent Stem Cell Dependence on the Repair of Endogenous DNA Damage.
New
Wells et al., Cincinnati, United States. In Stem Cell Reports, Feb 2016
Mechanistic studies revealed that G2-phase FA-deficient iPSCs possess large γH2AX-RAD51 foci indicative of accrued DNA damage, which correlated with activated DNA-damage signaling through CHK1.
CHK2 stability is regulated by the E3 ubiquitin ligase SIAH2.
New
Calzado et al., Córdoba, Spain. In Oncogene, Feb 2016
UNASSIGNED: The serine threonine checkpoint kinase 2 (CHK2) is a critical protein involved in the DNA damage-response pathway, which is activated by phosphorylation inducing cellular response such as DNA repair, cell-cycle regulation or apoptosis.
A synthetic lethal screen identifies ATR-inhibition as a novel therapeutic approach for POLD1-deficient cancers.
New
Gallmeier et al., München, Germany. In Oncotarget, Feb 2016
ATR-dependent POLD1 knockdown-induced cell killing was reproducible pharmacologically in POLD1-depleted DLD1 cells and a panel of other colorectal cancer cell lines by using chemical inhibitors of ATR or its major effector kinase CHK1.
The cytotoxic T cell proteome and its shaping by the kinase mTOR.
New
Impact
Cantrell et al., Cambridge, United Kingdom. In Nat Immunol, Jan 2016
We used high-resolution mass spectrometry to map the cytotoxic T lymphocyte (CTL) proteome and the effect of the metabolic checkpoint kinase mTORC1 on CTLs.
Development of cell-cycle checkpoint therapy for solid tumors.
Review
New
Tamura, Tokyo, Japan. In Jpn J Clin Oncol, Dec 2015
These proteins include cyclin-dependent kinase, checkpoint kinase, WEE1 kinase, aurora kinase and polo-like kinase.
Increased activity of CHK enhances the radioresistance of MCF-7 breast cancer stem cells.
New
Jiang et al., Suzhou, China. In Oncol Lett, Dec 2015
DNA damage may lead to activation of the checkpoint kinase (CHK) signaling pathway, of which debromohymenialdisine (DBH) is a specific inhibitor.
AMPK Protects Leukemia-Initiating Cells in Myeloid Leukemias from Metabolic Stress in the Bone Marrow.
New
Impact
Nakada et al., Houston, United States. In Cell Stem Cell, Dec 2015
Here we show that AMP-activated protein kinase (AMPK), a metabolic checkpoint kinase, confers metabolic stress resistance to leukemia-initiating cells (LICs) and promotes leukemogenesis.
CHK1 and replicative stress in T-cell leukemia: Can an irreverent tumor suppressor end up playing the oncogene?
Review
New
Barata et al., Lisbon, Portugal. In Adv Biol Regul, Nov 2015
Checkpoint kinase 1 (CHK1) is a key component of the ATR-dependent DNA damage response pathway that protects cells from RS by preventing replication fork collapse and activating homologous DNA repair.
A Pleiotropic RNA-Binding Protein Controls Distinct Cell Cycle Checkpoints to Drive Resistance of p53-Defective Tumors to Chemotherapy.
New
Impact
Yaffe et al., Cambridge, United States. In Cancer Cell, Nov 2015
Like p53, hnRNPA0 is activated by a checkpoint kinase (MK2) and simultaneously controls both cell cycle checkpoints through distinct target mRNAs, but unlike p53, this is through the post-transcriptional stabilization of p27(Kip1) and Gadd45α mRNAs.
DNA damage control: regulation and functions of checkpoint kinase 1.
Review
New
Gillespie et al., Santa Cruz de Tenerife, Spain. In Febs J, Oct 2015
Checkpoint kinase 1 (Chk1) is a master regulator of the DNA damage and replication checkpoints in vertebrate cells.
DNA damage in cancer therapeutics: a boon or a curse?
Review
New
Khanna, Sydney, Australia. In Cancer Res, Jul 2015
The tumor-suppressive role of DDR activity during the premalignant stage has been studied, and strong evidence is emerging for an oncogenic role for DDR proteins such as DNA-PK and CHK1 during the later stages of tumor development.
IL-37 requires the receptors IL-18Rα and IL-1R8 (SIGIRR) to carry out its multifaceted anti-inflammatory program upon innate signal transduction.
New
Impact
Nold et al., Melbourne, Australia. In Nat Immunol, Apr 2015
Furthermore, IL-37-IL-1R8 exerted a pseudo-starvational effect on the metabolic checkpoint kinase mTOR.
Phase I dose-escalation trial of checkpoint kinase 1 inhibitor MK-8776 as monotherapy and in combination with gemcitabine in patients with advanced solid tumors.
New
Impact
Munster et al., Santa Monica, United States. In J Clin Oncol, Apr 2015
PURPOSE: We determined the safety, pharmacokinetics, pharmacodynamics, and recommended phase II dose of MK-8776 (SCH 900776), a potent, selective checkpoint kinase 1 (Chk1) inhibitor, as monotherapy and in combination with gemcitabine in a first-in-human phase I clinical trial in patients with advanced solid tumor malignancies.
Hsp90: A New Player in DNA Repair?
Review
di Masi et al., Roma, Italy. In Biomolecules, 2014
Multiple components of the DNA double strand breaks repair machinery, including BRCA1, BRCA2, CHK1, DNA-PKcs, FANCA, and the MRE11/RAD50/NBN complex, have been described to be client proteins of Hsp90, which acts as a regulator of the diverse DDR pathways.
Autoregulatory mechanisms of phosphorylation of checkpoint kinase 1.
GeneRIF
Zhang et al., Cleveland, United States. In Cancer Res, 2012
Unique regulatory mechanisms of Chk1 phosphorylation; this suggests that expression of constitutively active Chk1 may represent a novel strategy to suppress tumor growth.
Coupling cellular localization and function of checkpoint kinase 1 (Chk1) in checkpoints and cell viability.
GeneRIF
Zhang et al., Cleveland, United States. In J Biol Chem, 2012
novel regulatory mechanisms that couple protein cellular localization with the checkpoint response and cell viability of Chk1.
ATP depletion triggers acute myeloid leukemia differentiation through an ATR/Chk1 protein-dependent and p53 protein-independent pathway.
GeneRIF
Wald et al., Cleveland, United States. In J Biol Chem, 2012
Data indicate that acute myeloid leukemia differentiation can be induced through ATP depletion and the subsequent activation of DNA damage signaling through an ATR/Chk1-dependent and p53-independent pathway.
Phosphorylation-dependent interactions between Crb2 and Chk1 are essential for DNA damage checkpoint.
GeneRIF
Du et al., Beijing, China. In Plos Genet, 2012
These results suggest that the main role of Crb2 in DNA damage checkpoint signaling is recruiting Chk1 to sites of DNA lesions.
CHK1 targets spleen tyrosine kinase (L) for proteolysis in hepatocellular carcinoma.
GeneRIF
Kang et al., China. In J Clin Invest, 2012
CHK1 phosphorylation of SYK(L) promoted its subsequent proteasomal degradation.
More papers using Chk1 antibodies
Inflammatory lipid mediators in ischemic retinopathy.
Supplier
Karl Mike, In PLoS ONE, 2004
... (9701) from Cell Signaling; protein A/G plus agarose and antibodies against PAF receptor (sc-8744) and CHK1 were from Santa Cruz Biotechnology; and antibody against phospho-histone ...
Statistics of the Comet assay: a key to discriminate between genotoxic effects
Supplier
Haber James E, In PLoS Biology, 2002
... Rabbit anti-Chk1 (#2345) and rabbit anti-Chk1-Ser345p (#2341) antibodies were from Cell Signaling, the mouse anti–β-actin (ab8226) ...
Functional interaction between BLM helicase and 53BP1 in a Chk1-mediated pathway during S-phase arrest
Supplier
Harris Curtis C. et al., In The Journal of Cell Biology, 2001
... Anti-Chk1: rabbit polyclonal #2345 (Cell Signaling).
Cytokine-driven cell cycling is mediated through Cdc25A
Supplier
Durum Scott K. et al., In The Journal of Cell Biology, 1999
... Inhibition of Chk1 was achieved by introducing chemically synthesized small interfering RNA (Santa Cruz Biotechnology, Inc.) using a lipid reagent, ...
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