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Cell division cycle 45 homolog

Cdc45, Cdc45p, CDC45L
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010] (from NCBI)
Top mentioned proteins: PCNA, POLYMERASE, MCM2, MCM5, CAN
Papers using Cdc45 antibodies
Levels of major selenoproteins in T cells decrease during HIV infection and low molecular mass selenium compounds increase.
Blagosklonny Mikhail, In PLoS ONE, 1998
... and antibodies against PCNA and Cdc45 were from Santa Cruz Biotechnology, Inc. ...
Papers on Cdc45
BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation.
Groth et al., Copenhagen, Denmark. In Embo J, Feb 2016
CDC45 recruitment, but not MCM2-7 loading, is impaired in BRPF3-depleted cells, identifying a BRPF3-dependent function of HBO1 in origin activation that is complementary to its role in licencing.
Cell culture density affects the proliferation activity of human adipose tissue stem cells.
Yoo et al., Seoul, South Korea. In Cell Biochem Funct, Feb 2016
Furthermore, expression of proliferation-associated genes, such as CDC45L, CDC20A and KIF20A, in P4 AT-MSCs was higher in CC1 than in CC2, and this difference was also observed in P12 AT-MSCs.
Exposure to fluorescent light triggers down regulation of genes involved with mitotic progression in Xiphophorus skin.
Savage et al., San Marcos, United States. In Comp Biochem Physiol C Toxicol Pharmacol, Dec 2015
Exposure to FL also resulted in down-regulated transcription of many genes involved with cell cycle progression (e.g., cdc20, cdc45, cdca7b, plk1, cdk1, ccnb-3, and cdca7a) and chromosome segregation (e.g., cenpe, cenpf, cenpi, cenpk, cenpo, cenpp, and cenpu; cep70; knstrm, kntc, mcm2, mcm5; smc2).
Analysis of the Histone H3.1 Interactome: A Suitable Chaperone for the Right Event.
Reinberg et al., New York City, United States. In Mol Cell, Dec 2015
The sNASP and ASF1 chaperones play pivotal roles in the processing of soluble histones but do not associate with the active CDC45/MCM2-7/GINS (CMG) replicative helicase.
Reduced cohesin destabilizes high-level gene amplification by disrupting pre-replication complex bindings in human cancers with chromosomal instability.
Kim et al., Seoul, South Korea. In Nucleic Acids Res, Oct 2015
After several passages, cohesin depletion inhibits DNA replication initiation by reducing the recruitment of pre-replication complexes such as minichromosome maintenance subunits 7 (MCM7), DNA polymerase α, and CDC45 at replication origins near the amplified regions, and as a result, decreases the DNA copy numbers of highly amplified genes.
The mechanism of DNA replication termination in vertebrates.
Walter et al., Boston, United States. In Nature, Oct 2015
Dissociation of the replicative CMG helicase (comprising CDC45, MCM2-7 and GINS) occurs only after the final ligation step, and is not required for completion of DNA synthesis, strongly suggesting that converging CMGs pass one another and dissociate from double-stranded DNA.
Identification and comparison of gonadal transcripts of testis and ovary of adult common carp Cyprinus carpio using suppression subtractive hybridization.
Chang et al., Xinxiang, China. In Theriogenology, Jun 2015
ZP3C and Psmb2 were expressed exclusively in ovary, whereas the expression levels of IFIPGL-1, Setd6, ATP-6, CDC45, AIF-1, and an unknown gene from the Ccfh2 clone were more strongly expressed in ovary than in testis.
ATM, ATR and DNA-PKcs expressions correlate to adverse clinical outcomes in epithelial ovarian cancers.
Madhusudan et al., Nottingham, United Kingdom. In Bba Clin, 2014
METHODS: We investigated ATM, ATR, and DNA-PKcs expressions in ovarian epithelial cancers [protein expression (n = 194 patients), mRNA expression (n = 156 patients)] and correlated to clinicopathological outcomes as well as expression of X-ray repair cross-complementing protein 1 (XRCC1), cell division cycle-45 (CDC45), cyclin-dependent kinase 1(CDK1) and Ki-67 in tumours.
Properties of the human Cdc45/Mcm2-7/GINS helicase complex and its action with DNA polymerase epsilon in rolling circle DNA synthesis.
Hurwitz et al., New York City, United States. In Proc Natl Acad Sci U S A, 2012
Data show that using a 200-nt primed circular DNA substrate, the combined action of DNA polymerase epsilon and the Cdc45/Mcm2-7/GINS (CMG complex) leads to the formation of products >10 kb in length.
Structural and functional insights into the DNA replication factor Cdc45 reveal an evolutionary relationship to the DHH family of phosphoesterases.
Onesti et al., Trieste, Italy. In J Biol Chem, 2012
Structural and functional insights into the DNA replication factor Cdc45 reveal an evolutionary relationship to the DHH family of phosphoesterases
RAD51- and MRE11-dependent reassembly of uncoupled CMG helicase complex at collapsed replication forks.
Costanzo et al., London, United Kingdom. In Nat Struct Mol Biol, 2012
Data show that MRE11- and RAD51-dependent fork repair leading to reloading of the GINS onto the MCM-CDC45 complex still engaged with the DNA could be sufficient to restore a functional CDC45-MCM-GINS (CMG) helicase complex.
Origin association of Sld3, Sld7, and Cdc45 proteins is a key step for determination of origin-firing timing.
Araki et al., Mishima, Japan. In Curr Biol, 2012
The low-abundance replication proteins Sld3, Sld7, and Cdc45 form a complex and associate with the early-firing origins in G1 phase in a manner that depends on Dbf4-dependent kinase (DDK), which is essential for the initiation of DNA replication.
Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy.
Nasheuer et al., Galway, Ireland. In Plos One, 2011
After UV-damage, Cdc45 is still present in a large multi-protein complex and that its mobility within living cells is consistently similar following UVC-mediated DNA damage.
Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint.
Hsieh et al., Saint Louis, United States. In Nature, 2010
Stabilized MLL protein accumulates on chromatin, methylates histone H3 lysine 4 at late replication origins and inhibits the loading of CDC45 to delay DNA replication.
CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast.
Araki et al., Mishima, Japan. In Nature, 2007
Here we show that both an allele of CDC45 (JET1) and high-copy DPB11, in combination with Sld2-11D, separately confer CDK-independent DNA replication.
Regulation of eukaryotic DNA replication at the initiation step.
Nasheuer et al., Jena, Germany. In Biochem Soc Trans, 2003
Two factors, Cdc45p (cell division cycle 45p) and DNA polymerase alpha-primase, are necessary in this process.
Initiation of DNA replication in eukaryotes is an intriguing cascade of protein interactions.
Abramova et al., Moscow, Russia. In Biochemistry (mosc), 2002
A chain of interesting interactions between Orc1p-6p, Cdc6p, Mcm2p-7p, Mcm10p, Cdt1, Cdc45p, Dbf4/Cdc7p, RPA, and DNA polymerase alpha takes place during this period.
Mechanisms of DNA topoisomerase I-induced cell killing in the yeast Saccharomyces cerevisiae.
Bjornsti et al., Memphis, United States. In Ann N Y Acad Sci, 1999
These include genes encoding essential DNA replication proteins (CDC45 and DPB11) and proteins involved in SUMO- or ubiquitination (UBC9 and DOA4).
Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin.
Stillman et al., United States. In Science, 1998
Cdc45p, a protein essential for initiation of DNA replication, associates with chromatin after "start" in late G1 and during the S phase of the cell cycle.
Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
Bell et al., Cambridge, United States. In Cell, 1997
We demonstrate that ORC, Cdc45p, and MCM proteins are components of prereplication complexes (pre-RC).
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