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CD4 molecule

CD4
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010] (from NCBI)
Top mentioned proteins: CD8, HAD, CAN, AGE, Foxp3
Papers using CD4 antibodies
Morphofunctional fundamentals for peptide regulation of aging
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Kvetnoy I. M. et al., In Journal of Amino Acids, 2010
... and the monoclonal antibodies to CD4, marked with phycoerytherin (Becton Dickinson, USA) ...
Dietary sulfur amino acid effects on fasting plasma cysteine/cystine redox potential in humans
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James S. Jill et al., In Autism Research and Treatment, 2010
... Human Monocyte Isolation Kit II and Human CD4 T cell Isolation Kit II were purchased from Miltenyi Biotec (Bergisch-Gladbach, Germany) ...
IL-21 regulates germinal center B cell differentiation and proliferation through a B cell-intrinsic mechanism
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Brooks David G. et al., In The Journal of Experimental Medicine, 2009
... LCMV-specific CD4 TCR transgenic ...
Primary Malignant Giant-Cell Tumor of Bone Has High Survival Rate
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Gitelis Steven et al., In Diagnostic Pathology, 2009
... following antibodies were used on Ventana Benchmark and the manufacturer's solution CC1 for antigen retrieval.: CD4 (Biocare Medical, Concord, CA) 1:10 dilution, ...
The HIV-1 central polypurine tract functions as a second line of defense against APOBEC3G/F.
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Vij Neeraj, In PLoS ONE, 2009
... astrocytic cell line, SVGA-LTR GFP or -LTR-gag-GFP RRE reporter cells, 293 HEK cells, and Magi (CD4/CCR5) cells [47] were all maintained in DMEM (Gibco BRL life technologies, NY, USA) containing 10% ...
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Papers on CD4
Loss of RUNX1/AML1 arginine-methylation impairs peripheral T cell homeostasis.
New
Okuda et al., Kyoto, Japan. In Br J Haematol, 26 Jun 2015
However, Runx1(KTAMK) (/) (KTAMK) mice showed a reduction in CD3(+) T lymphoid cells and a decrease in CD4(+) T cells in peripheral lymphoid organs, in comparison to their wild-type littermates, leading to a reduction in the CD4(+) to CD8(+) T-cell ratio.
The interbranchial lymphoid tissue of Atlantic Salmon (Salmo salar L) extends as a diffuse mucosal lymphoid tissue throughout the trailing edge of the gill filament.
New
Koppang et al., Oslo, Norway. In J Morphol, 26 Jun 2015
Only a few of these cells were identified as CD8α(+) -cells, and there was a significantly (P < 0.05) higher relative expression of CD4- than of CD8- related genes in all gill segments investigated.
CD4(+) Foxp3(+) regulatory T-cell number increases in the gastric tissue of C57BL/6 mice infected with Helicobacter pylori.
New
Zhang et al., Hengyang, China. In Apmis, 22 Jun 2015
The aim of this study was to investigate the number and localization of CD4(+) Foxp3(+) Treg cells in stomachs and spleens of H. pylori-infected mice.
Structural Repertoire of HIV-1-Neutralizing Antibodies Targeting the CD4 Supersite in 14 Donors.
New
Impact
Kwong et al., Bethesda, United States. In Cell, 20 Jun 2015
UNASSIGNED: The site on the HIV-1 gp120 glycoprotein that binds the CD4 receptor is recognized by broadly reactive antibodies, several of which neutralize over 90% of HIV-1 strains.
Phagocyte subsets and lymphocyte clonal deletion behind ineffective immune response to Staphylococcus aureus.
Review
New
Bagnoli et al., Siena, Italy. In Fems Microbiol Rev, 19 Jun 2015
Indeed, low CD4(+) T-cell counts represent an important risk factor for skin infections and bacteraemia in patients.
Emtricitabine/rilpivirine/tenofovir disoproxil fumarate for the treatment of HIV-1 infection in adults.
Review
New
Ramadan et al., Jbail, Lebanon. In J Infect Public Health, 19 Jun 2015
Complera™ remains an acceptable alternative treatment to Atripla™ in ART naïve patients who have a pre-ART plasma HIV RNA <100,000copies/mL and CD4 count >200cells/mm(3) with non-inferior efficacy and better safety and tolerability.
Epigenetic Dynamics During Cd4(+) T Cells Lineage Commitment.
Review
New
Suarez-Alvarez et al., Oviedo, Spain. In Int J Biochem Cell Biol, 11 Jun 2015
Here we review the contribution of epigenetic mechanisms to transcriptional control during CD4 T cell differentiation and the ways in which these mechanisms interact with key transcription factors to ensure proper maturation and maintenance of cell identity.
Mutant MHC class II epitopes drive therapeutic immune responses to cancer.
New
Impact
Sahin et al., Mainz, Germany. In Nature, 30 May 2015
Here we show in three independent murine tumour models that a considerable fraction of non-synonymous cancer mutations is immunogenic and that, unexpectedly, the majority of the immunogenic mutanome is recognized by CD4(+) T cells.
Th17 cells transdifferentiate into regulatory T cells during resolution of inflammation.
New
Impact
Flavell et al., New Haven, United States. In Nature, 29 May 2015
Here we used two new fate-mapping mouse models to track Th17 cells during immune responses to show that CD4(+) T cells that formerly expressed IL-17A go on to acquire an anti-inflammatory phenotype.
Toxicities of Immunotherapy for the Practitioner.
Review
New
Impact
Disis et al., Bethesda, United States. In J Clin Oncol, 27 May 2015
The basis for the majority of these adverse events is a hyperactivated T-cell response with reactivity directed against normal tissue, resulting in the generation of high levels of CD4 T-helper cell cytokines or increased migration of cytolytic CD8 T cells within normal tissues.
Group 3 innate lymphoid cells mediate intestinal selection of commensal bacteria-specific CD4+ T cells.
New
Impact
Sonnenberg et al., New York City, United States. In Science, 23 May 2015
UNASSIGNED: Inflammatory CD4(+) T cell responses to self or commensal bacteria underlie the pathogenesis of autoimmunity and inflammatory bowel disease (IBD), respectively.
The postoperative clinical outcomes and safety of early enteral nutrition in operated gastric cancer patients.
New
Jia et al., Beijing, China. In J Buon, Mar 2015
The activities of multiple immune cell types, including CD3(+), CD4(+), CD4(+)/CD8(+), and natural killer (NK) cells, were significantly lower in both groups on postoperative day 1 when compared with the preoperative levels (p<0.05).
Epigenetic Control of Interferon-Gamma Expression in CD8 T Cells.
Review
New
Viola et al., Rio de Janeiro, Brazil. In J Immunol Res, Dec 2014
IFN-γ expression by CD4 T lymphocytes is observed only after T helper (Th) 1 differentiation and there are several studies about the molecular mechanisms that control Ifng expression in these cells.
Twelve-Month Antiretroviral Therapy Suppresses Plasma and Genital Viral Loads but Fails to Alter Genital Levels of Cytokines, in a Cohort of HIV-Infected Rwandan Women.
New
van de Wijgert et al., Amsterdam, Netherlands. In Plos One, Dec 2014
MATERIALS AND METHODS: All participants were evaluated for GVL, plasma viral load (PVL), CD4 count, various sexually-transmitted infections (STIs) at baseline and at month 12. Genital concentration of 19 cytokines and mRNA expression of APOBEC3G and BST2, two host HIV restriction factors, were evaluated at baseline in all participants.
Recombinatorial biases and convergent recombination determine interindividual TCRβ sharing in murine thymocytes.
GeneRIF
Han et al., Xiamen, China. In J Immunol, 2012
Thymic selection does not appear to play an important role in CD4+CD8+ T cell receptor (TCR)beta repertoire overlap between individuals.
CD4+ T cell-dependent IFN-γ production by CD8+ effector T cells in Mycobacterium tuberculosis infection.
GeneRIF
Ernst et al., New York City, United States. In J Immunol, 2012
CD4-positive cell deficiency impairs IFN-gamma production by CD8-positive effector cells at the site of Mycobacterium tuberculosis infection in mice.
Lineage-specific differences between human and simian immunodeficiency virus regulation of gp120 trimer association and CD4 binding.
GeneRIF
Sodroski et al., Boston, United States. In J Virol, 2012
The N/C termini and the inner domain beta-sandwich and layer 2 support the noncovalent association of gp120 trimer with the CD4.
Intrinsic impairment of CD4(+)CD25(+) regulatory T cells in acquired aplastic anemia.
GeneRIF
Zheng et al., Tianjin, China. In Blood, 2012
The study provides evidence for impairment of CD4(+)CD25(+) regulatory T cells -including decreased frequencies in peripheral blood and bone marrow in patients with in acquired aplastic anemia.
Foxp3+CD25(high) CD4+ regulatory T cells from indeterminate patients with Chagas disease can suppress the effector cells and cytokines and reveal altered correlations with disease severity.
GeneRIF
Gomes et al., Belo Horizonte, Brazil. In Immunobiology, 2012
Data indicate that the frequency of Foxp3(+)CD25(high) CD4(+)-T cells is augmented and correlated with the maintenance of a better cardiac function in indeterminate (IND) Chagas disease.
Role of regulatory T cells in rheumatoid arthritis: facts and hypothesis.
Review
Gerli et al., Perugia, Italy. In Auto Immun Highlights, 2010
Regulatory T cells (Treg) are a CD4(+) lymphocyte subset involved in self-tolerance and autoimmunity prevention.
More papers using CD4 antibodies
CD4+ T lymphocytes mediate acute pulmonary ischemia-reperfusion injury.
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Wherry E. John, In PLoS Pathogens, 2008
... fl/fl mice to CD4-cre transgenic mice ...
A novel regulatory B-cell population in sheep Peyer's patches spontaneously secretes IL-10 and downregulates TLR9-induced IFNalpha responses.
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Tailleux Ludovic, In PLoS ONE, 2008
... rat anti CD8α clone YTS105.18 and hamster anti-CD11c clone HL3 were purchased from Serotec, rat anti-CD4 clone YTS191.1.2
Human immunodeficiency virus: 25 years of diagnostic and therapeutic strategies and their impact on hepatitis B and C virus
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Gürtler Lutz G. et al., In Medical Microbiology and Immunology, 2008
... CD4 cells were measured by FACS analysis (Becton–Dickinson, Heidelberg, Germany).RNA was extracted from EDTA-plasma according to the recommendation of the manufacturer (Qiagen, Hilden, Germany) ...
Improved expression and reactivity of transduced tumor-specific TCRs in human lymphocytes by specific silencing of endogenous TCR
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Hooijberg Erik et al., In Journal of Translational Medicine, 2008
... For this purpose, total PBMC were stained with anti-CD4 Ab and microbead-conjugated anti-mouse IgG Abs (Miltenyi Biotec), followed by MACS sorting ...
Neuromodulatory activities of CD4+CD25+ regulatory T cells in a murine model of HIV-1-associated neurodegeneration
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Unutmaz Derya, In PLoS ONE, 2008
... cell population by negative selection (CD4 separation kit, Miltenyi Biotec), as described above ...
Visualizing antigen specific CD4+ T cells using MHC class II tetramers
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Nepom G T et al., In Genes and Immunity, 2008
... In the first step CD4+ T cells were separated from all non-CD4 cells using CD4+ T cell isolation kit (Miltenyi Biotec, Auburn, CA, USA).
Interaction of human PD-L1 and B7-1
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Ohashi Kazuhiko et al., In Veterinary Research, 2007
... Separation System (BD Biosciences, Franklin Lakes, NJ, USA) and the following antibodies: CACT138A (mouse anti-bovine CD4: VMRD, WA, USA), IL-A51 (mouse ...
CD8+ CD205+ splenic dendritic cells are specialized to induce Foxp3+ regulatory T cells.
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Zimmer Jacques, In PLoS ONE, 2007
... OT II OVA CD4 transgenic mice were kindly provided ...
SERS-Based Detection in an Optofluidic Ring Resonator Platform
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Fan Xudong et al., In Sensors (Basel, Switzerland), 2006
... CD4 mouse monoclonal IgG antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and ...
KLRG1 binds cadherins and preferentially associates with SHIP-1
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Rishi Arun, In PLoS ONE, 2006
... Anti-CD3 and anti-CD4 (clones OKT3 and OKT4; eBioscience, San Diego, CA) stimulation ...
Semen-derived amyloid fibrils drastically enhance HIV infection.
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KewalRamani Vineet N., In PLoS ONE, 2006
... to the manufacturer's guidelines for phycoerythrin (PE)-conjugated anti-CD8 (clone DK25; BD Pharmingen), fluorescein isothiocyanate (FITC)-conjugated anti-CD4 (clone L200; Dako), peridinin-chlorophyll-Cychrome (PerCP-Cy5.5)-conjugated
Treatment Outcomes of Patients with HIV and Tuberculosis
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Risbud A.R. et al., In The Indian Journal of Medical Research, 2006
... To determine the degree of immune-suppression in the enrolled patients, the CD4 counts were estimated in freshly collected blood in EDTA containing vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ, USA) ...
Cutting Edge: Influenza A virus activates TLR3-dependent inflammatory and RIG-I-dependent antiviral responses in human lung epithelial cells.
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Emerman Michael, In PLoS Pathogens, 2006
... 293T-4X4 and 293T CXCR4 were generated using lentiviral transduction of 293T with lentiviruses coding for CD4 and/or CXCR4 molecules and magnetic sorting (Miltenyi Biotec).
An anti-CD45RO/RB monoclonal antibody modulates T cell responses via induction of apoptosis and generation of regulatory T cells.
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Rénia Laurent, In PLoS ONE, 2004
... Leukocytes were labelled with a combination of antimurine CD4-allophycocyanin (rat IgG2a, clone RM4-5, BD Biosciences), CD19-PE-Cyanin7 (rat IgG2a, clone ...
Cytotoxic T-cell non-Hodgkin's lymphoma of the thyroid gland
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Lee Young-Don et al., In Journal of Korean Medical Science, 2004
... by avidin-biotin-peroxidase complex methods using antibodies against CD3 (Dako, Glostrup, Denmark, prediluted), CD20 (Dako, prediluted), CD4 (Dako, prediluted), CD8 (Dako, prediluted), ...
The Tim-3 ligand galectin-9 negatively regulates T helper type 1 immunity
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Behar Samuel M. et al., In The Journal of Experimental Medicine, 2004
... Kuchroo), goat F(ab’)2 anti–human Ig (2012–01; SouthernBiotech), CD11b microbeads (Miltenyi Biotec), CD4 microbeads (Miltenyi Biotec), human IgG1κ (I5154; Sigma-Aldrich), ...
Extrapulmonary tuberculosis
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Gulati Vinay et al., In Clinical and Developmental Immunology, 2003
... CD4 count and HIV viral load were estimated using flow-cytometry (Becton Dickinson, USA) and Amplicor HIV-1 ...
MOZ-TIF2, but not BCR-ABL, confers properties of leukemic stem cells to committed murine hematopoietic progenitors.
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Alberola-Ila Jose, In PLoS ONE, 2003
... stained with the following antibodies, all conjugated to PE-Cy5: CD3ε (145-2C11, BioLegend, San Diego, CA), CD4 (RM4-5, eBioscience, San Diego, CA), CD8α ...
Randomized study of high-dose and low-dose interleukin-2 in patients with metastatic renal cancer
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Nakajima Y et al., In British Journal of Cancer, 2002
... The sections were then incubated overnight at 4°C with anti-human CD45RO (UHL1, monoclonal mouse; DAKO), CD4 (1 : 40) (4B12, monoclonal mouse; DAKO), and CD8 (C8/144B, monoclonal ...
The influence of the src-family kinases, Lck and Fyn, on T cell differentiation, survival and activation
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Jameson Stephen C. et al., In The Journal of Experimental Medicine, 2002
... thank Alfred Singer and Tom McCaughtry (National Institutes of Health) for generously providing cells from E8I-CD4 transgenic mice, and also Hidde ...
IL-7 Promotes the Transition of CD4 Effectors to Persistent Memory Cells
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Swain Susan L. et al., In The Journal of Experimental Medicine, 2002
... We used naive CD4 T cells from AND mice transgenic (Tg) for a TCR specific ...
The regulation of SIRT2 function by cyclin-dependent kinases affects cell motility
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Lüscher Bernhard et al., In The Journal of Cell Biology, 2002
... α-tubulin acetylation in cells, HEK293 cells were cotransfected with plasmids expressing SIRT2 and the human CD4 antigen with a truncated cytoplasmic domain (pMACS4.1;
Phosphoinositide regulation of the actin cytoskeleton
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Yin Helen L. et al., In The Journal of Cell Biology, 2002
allophycocyanin (APC)–anti-CD4, and anti-FcγRIIB/III mAb 2.4G2 (BD Biosciences); anti-pERK (Cell Signaling Technology); ...
A DNA vector-based RNAi technology to suppress gene expression in mammalian cells.
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Unutmaz Derya, In PLoS ONE, 2001
... mice with CD4-Cre transgenic mice, in which the ...
Costimulation via OX40L Expressed by B Cells Is Sufficient to Determine the Extent of Primary CD4 Cell Expansion and Th2 Cytokine Secretion In Vivo
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Bradley Linda M. et al., In The Journal of Experimental Medicine, 2001
... OT-II TCR (vα2, vβ5) CD4 transgenic mice that respond to ...
Interleukin 1 Receptor Dependence of Serum Transferred Arthritis Can be Circumvented by Toll-like Receptor 4 Signaling
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Corr Maripat et al., In The Journal of Experimental Medicine, 2001
... TCR were identified at 2–3 wk of age by cytofluorometry of peripheral blood lymphocytes using anti-CD4 PE (Caltag) and anti-Vβ6 FITC (BD Biosciences) labeled antibodies ...
A Bayesian framework for the analysis of microarray expression data: regularized t-test and statistical inferences of gene changes.
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Kosik Kenneth S et al., In Genome Biology, 2000
... Naïve CD4 cells were purified from spleen and lymph nodes of Tcrα-/- DO11 TCR transgenic mice by magnetic bead ...
Homeostatic Competition Among T Cells Revealed by Conditional Inactivation of the Mouse Cd4 Gene
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Killeen Nigel et al., In The Journal of Experimental Medicine, 2000
... Cd4 mutation.The generation of dLck-hcre transgenic mice expressing a codon optimized ...
Human foamy virus capsid formation requires an interaction domain in the N terminus of Gag
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Young John A. T, In PLoS Pathogens, 2000
... CD4-positive T cells were negatively selected using the CD4-positive T Cell Isolation Kit II (Miltenyi Biotec, ...
CD1d-expressing Dendritic Cells but Not Thymic Epithelial Cells Can Mediate Negative Selection of NKT Cells
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Wang Chyung-Ru et al., In The Journal of Experimental Medicine, 1999
and NK1.1 (PK136); Cy-Chrome-conjugated mAbs specific for TCRβ (H57–597), and CD4 (RM4–5; BD Biosciences).
Involvement of Avidity for Major Histocompatibility Complex in Homeostasis of Naive and Memory T Cells
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Stockinger Brigitta et al., In The Journal of Experimental Medicine, 1999
... CD4 overexpression in A18 TCR-transgenic mice (4A18 mice) was ...
Highly resolved chemical imaging of living cells by using synchrotron infrared microspectrometry
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Ko Fu-Hsiang et al., In International Journal of Nanomedicine, 1997
... Anti-human CD4 (14-0049) and biotinylated anti-human CD3 (13-0038) antibodies were purchased from eBioscience, Inc (San Diego, CA) ...
Platelet-derived endothelial cell growth factor/thymidine phosphorylase immunohistochemical expression in lymphoid tissue and lymphoid malignancies
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Hou J. Steve et al., In Advances in Hematology, 1996
... antibodies used in this study were TP (1 : 1000; Novus Biologicals, Littleton, Colo), CD3 (1 : 100; Dako), CD4 (1 : 10; Dako), CD68 (1 : 2000; Dako), and ...
Transcription factors of the NFAT familyregulation and function
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Paul William E. et al., In The Journal of Experimental Medicine, 1996
... T cells were purified by positive selection (through an automatic MACS machine) using anti–mouse CD4 (L3T4) MicroBeads (Miltenyi Biotec).
Transgenic expression of CD95 ligand on islet β cells induces a granulocytic infiltration but does not confer immune privilege upon islet allografts
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Ricciardi-Castagnoli Paola et al., In The Journal of Experimental Medicine, 1996
... purified from TCR-OVA DO11.10 transgenic mice, bred in BALB/c background, by positive selection with an anti-CD4 antibody coupled to magnetic microbeads, using MiniMacS Separating columns (all from Miltenyi Biotec).
Prevalence of intestinal parasites in adult patients with enteropathic. AIDS in North-eastern Tanzania.
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Wiwanitkit Viroj, In BMC Gastroenterology, 1995
... The CD4 count determination in this study was performed by automated flow cytometry analyzer, FACS Calibur (Becton Dickinson).
Identification of a major co-receptor for primary isolates of HIV-1
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Liu Yong-Jun et al., In The Journal of Experimental Medicine, 1995
... Dickinson) and CD45RA-Tricolor (MEM 56; Caltag), and in addition, different PE-conjugated antibodies: anti–HLA-DR (Becton Dickinson), CD4 (Leu-3a; Becton Dickinson), and IL-3Rα (9F5l; BD ...
Interleukin 7 Regulates the Survival and Generation of Memory CD4 Cells
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Bradley Linda M. et al., In The Journal of Experimental Medicine, 1994
... OT-II TCR (vα2 and vβ5) CD4 transgenic mice that respond to ...
Existence of a soluble form of CD50 (intercellular adhesion molecule-3) produced upon human lymphocyte activation. Present in normal human serum and levels are increased in the serum of systemic lupus erythematosus patients
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Blanco Julià et al., In Retrovirology, 1994
... Whole CD4 T cells, memory CD4 T cells (CD45RO+) and naïve CD4 T cells (CD45RA+) were purified by negative immunomagnetic selection using the appropriate cocktails of antibodies (Miltenyi Biotec SL, Madrid, Spain) ...
A lineage-specific transcriptional silencer regulates CD4 gene expression during T lymphocyte development
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Perlmutter Roger M. et al., In The Journal of Experimental Medicine, 1993
... The 727 CD4 transgenic mouse line has previously ...
Changes in interleukin-2 and interleukin-4 production in asymptomatic, human immunodeficiency virus–seropositive individuals
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Reiner Steven L. et al., In The Journal of Experimental Medicine, 1992
... CD4° mice and CD4 transgenic mice were also bred ...
Intestinal Epithelial Cells Synthesize Glucocorticoids and Regulate T Cell Activation
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Brunner Thomas et al., In The Journal of Experimental Medicine, 1990
... Anti–CD69-FITC, anti–TCRβ-FITC, anti–CD8α-Cy-chrome, anti–CD8β-PE, anti–CD4-PE, and anti–αEβ7-PE were obtained from BD Biosciences.
Gamma delta beta-thalassemia due to a de novo mutation deleting the 5′ beta-globin gene activation-region hypersensitive sites
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Nakano Hideki et al., In The Journal of Experimental Medicine, 1988
... , biotinylated anti-B220, biotinylated anti-CD3, biotinylated anti–I-Ad, FITC-conjugated anti–I-Ad, PE-conjugated anti–I-Ad, and anti-CD4 (PharMingen); NLDC-145 and MOMA-1 (Bachem).
Precise gene fusion by PCR.
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Kanellopoulos Jean, In PLoS ONE, 1988
... T cells were negatively isolated using magnetic beads (CD4 isolation kit II, Miltenyi Biotec, Auburn, CA, purification >90%) ...
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