How to approach the many faces of endometrioid carcinoma.
Houston, United States. In Mod Pathol, Jan 2016
Variants with distinct architectural and cytologic features include the following: (1) ECa with a villoglandular pattern (tumor with finger-like papillae lined by bland cells with a tendency for vascular/lymphatic invasion and lymph node metastasis once this pattern is seen within the myoinvasive component); (2) papillary ECa of intermediate grade (grade 2) (tumor that can be mistaken for serous carcinoma, as it contains papillae showing slightly irregular contours, moderately atypical cells, and it is associated with vascular/lymphatic invasion/lymph node metastasis, but with common association with mucinous metaplasia, MELF (microcystic, elongated, and fragmented) pattern of invasion, and wild p53 expression); (3) ECa with non-villous papillae (tumor containing pseudopapillae within glands with bland-appearing cytology commonly associated with abortive squamous differentiation and otherwise not different from usual ECa); (4) ECa with microglandular-like pattern (tumor that mimics microglandular hyperplasia of the cervix, often lacking the typical appearance of microglandular hyperplasia and showing Ki-67 index >10%, strong CD10 expression, and negative PAX-2, p63, and CD34); and (5) ECa with sex cord-like formations and hyalinization (tumor with interconnected cords and nests of bland epithelioid and spindled cells that merge with a typical component of low-grade ECa, usually associated with squamous differentiation and hyalinization).
Homology-driven genome editing in hematopoietic stem and progenitor cells using ZFN mRNA and AAV6 donors.
More papers using
Richmond, United States. In Nat Biotechnol, Dec 2015
Here we report that combining electroporation of zinc finger nuclease (ZFN) mRNA with donor template delivery by adeno-associated virus (AAV) serotype 6 vectors directs efficient genome editing in HSPCs, achieving site-specific insertion of a GFP cassette at the CCR5 and AAVS1 loci in mobilized peripheral blood CD34(+) HSPCs at mean frequencies of 17% and 26%, respectively, and in fetal liver HSPCs at 19% and 43%, respectively.
MOZ-TIF2, but not BCR-ABL, confers properties of leukemic stem cells to committed murine hematopoietic progenitors.
In PLoS ONE, 2003
... Antibodies against c-Kit (APC-conjugated, 2B8, eBioscience), Sca-1 (PE-conjugated, D7, eBioscience) and CD34 (Pacific Blue conjugated, RAM34, eBioscience) were also used ... DAVID: Database for Annotation, Visualization, and Integrated Discovery
In Stem Cell Reviews, 2002
... Antibodies used were anti-K15 (Neomarkers, Lab Vision, Fremont, CA), anti-CD34 (clone RAM 34, eBioscience, San Diego, CA), anti ... Expansion of human NOD/SCID-repopulating cells by stem cell factor, Flk2/Flt3 ligand, thrombopoietin, IL-6, and soluble IL-6 receptor
In Advances in Hematology, 1999
... hematopoietic stem/progenitor cells were collected from human umbilical cord blood using a magnetic cell sorting system, MACS CD34 Isolation kit (Miltenyi Biotec Inc., Sunnyvale, Calif, USA), ...