Homology-driven genome editing in hematopoietic stem and progenitor cells using ZFN mRNA and AAV6 donors.
Richmond, United States. In Nat Biotechnol, Dec 2015
Here we report that combining electroporation of zinc finger nuclease (ZFN) mRNA with donor template delivery by adeno-associated virus (AAV) serotype 6 vectors directs efficient genome editing in HSPCs, achieving site-specific insertion of a GFP cassette at the CCR5 and AAVS1 loci in mobilized peripheral blood CD34(+) HSPCs at mean frequencies of 17% and 26%, respectively, and in fetal liver HSPCs at 19% and 43%, respectively.
Microcystic/Reticular Schwannoma of the Mandible First Case Report and Review of the Literature.
Shanghai, China. In Medicine (baltimore), Nov 2015
Tumor cells were also positive for CD34, CD99, and NSE, but negative for CK, EMA, CK5/6, P63, Calponin, CD10, SMA, Desmin, GFAP, NF, Syn, and CgA.
Novel Techniques for Ex Vivo Expansion of Cord Blood: Clinical Trials.
More papers using
Minneapolis, United States. In Front Med (lausanne), 2014
Many of these methods lead to substantial expansions of total nucleated cells and CD34(+) cells, and significantly improved time to neutrophil or platelet engraftment in patients transplanted with the expanded products compared to the recipients of unmanipulated CBT.
MOZ-TIF2, but not BCR-ABL, confers properties of leukemic stem cells to committed murine hematopoietic progenitors.
In PLoS ONE, 2003
... Antibodies against c-Kit (APC-conjugated, 2B8, eBioscience), Sca-1 (PE-conjugated, D7, eBioscience) and CD34 (Pacific Blue conjugated, RAM34, eBioscience) were also used ... DAVID: Database for Annotation, Visualization, and Integrated Discovery
In Stem Cell Reviews, 2002
... Antibodies used were anti-K15 (Neomarkers, Lab Vision, Fremont, CA), anti-CD34 (clone RAM 34, eBioscience, San Diego, CA), anti ... Expansion of human NOD/SCID-repopulating cells by stem cell factor, Flk2/Flt3 ligand, thrombopoietin, IL-6, and soluble IL-6 receptor
In Advances in Hematology, 1999
... hematopoietic stem/progenitor cells were collected from human umbilical cord blood using a magnetic cell sorting system, MACS CD34 Isolation kit (Miltenyi Biotec Inc., Sunnyvale, Calif, USA), ...