Amino-terminal arginylation as a degradation signal for selective autophagy.
South Korea. In Bmb Rep, Sep 2015
We showed that many ER residents, such as BiP, contain evolutionally conserved arginylation permissive pro-N-degrons, and that certain inducers like dsDNA or proteasome inhibitors cause their translocation into the cytoplasm where they bind misfolded proteins and undergo amino-terminal arginylation by arginyl transferase 1 (ATE1).
Assaying ATE1 Activity In Vitro.
Philadelphia, United States. In Methods Mol Biol, 2014
Here we describe a standard arginyltransferase assay in vitro using bacterially expressed purified ATE1 in a system with minimal number of components (Arg, tRNA, Arg-tRNA synthetase, and arginylation substrate).
Arginyltransferase: A Personal and Historical Perspective.
New York City, United States. In Methods Mol Biol, 2014
In the late 1960s and early 1970s, characterization of arginylation has been spearheaded via biochemical studies that enabled the first characterization of ATE1 and its substrate specificity.
Applying Arginylation for Bottom-Up Proteomics.
Zürich, Switzerland. In Methods Mol Biol, 2014
Arginylation is an enzymatic reaction in which arginyl-tRNA protein transferase 1 (ATE1, EC 126.96.36.199) conjugates a single arginyl moiety from aminoacylated tRNA(Arg) onto a target polypeptide.
Assay of Arginyltransferase Activity by a Fluorescent HPLC Method.
Sakado, Japan. In Methods Mol Biol, 2014
Syntheses of fluorescent substrate and product for arginyltransferase, N-aspartyl-N'-dansylamido-1,4-butanediamine (Asp-4DNS), and N-arginylaspartyl-N'-dansylamido-1,4-butanediamine (ArgAsp-4DNS), respectively, including their precursor 4-dansylamidobutylamine (4DNS), are described.
High-Throughput Arginylation Assay in Microplate Format.
Tezpur, India. In Methods Mol Biol, 2014
Here we describe the biochemical assay for ATE1-mediated arginylation in microplate format, which can be applied to high-throughput screens for identification of small-molecule inhibitors and activators of ATE1, high-volume analysis of ATE1 substrates, and other similar applications.