Mayfield et al., San Diego, United States. In Biotechnol J, Mar 2015
The improved arylsulfatase expression cassette was used to screen a new transgene promoter from the ARG7 gene, and found that the ARG7 promoter can express the ARS2 reporter as strongly as the HSP70-RBCS2 chimeric promoter that currently ranks as the best available promoter, thus adding to the list of useful nuclear promoters.
Slaninova et al., Regensburg, Germany. In Protist, 2007
Chlamydomonas reinhardtii arg7-8 (arg2) mutant strains carrying a hitherto undescribed mutation in their argininosuccinate lyase gene (ARG7) that leads to arginine auxotrophy have been used together with the corresponding wild-type gene as a very reliable transformation system since 1989.
Hegemann et al., Saint Petersburg, Russia. In Gene, 2001
After addition of the 5'-region of hsp70A as a second promoter and insertion of the rbcS2 intron I, the transformation rate increased to two transformants per 1 x 10(5) cells, which is close to the efficiency of transforming auxotrophic strains with the homologous marker arg7.
Loppes et al., Liège, Belgium. In Mol Gen Genet, 1998
Twenty-four phoN mutants lacking the derepressible neutral (DN) phosphatase activity were isolated following transformation of the cw15arg7 strain with plasmid pASL harbouring the ARG7 gene encoding argininosuccinate lyase.
Electroporation of strains CC3395 and CC425, cell wall-less mutants devoid of argininosuccinate lyase (encoded by ARG7), in the presence of the plasmid pJD67 (which contains ARG7) was used to optimize conditions for the introduction of exogenous DNA.