Deep sequencing with intronic capture enables identification of an APC exon 10 inversion in a patient with polyposis.
Seattle, United States. In Genet Med, 27 Apr 2014
We report the case of a 40-year-old woman with adenomatous colon polyps too numerous to count and who had a complex inversion spanning the entire exon 10 in APC (the gene encoding for adenomatous polyposis coli), causing exon skipping and resulting in a frameshift and premature protein truncation.Methods:In this study, we employed complete APC gene sequencing using high-coverage next-generation sequencing by ColoSeq, analysis with BreakDancer and SLOPE software, and confirmatory transcript analysis.Results:ColoSeq identified a complex small genomic rearrangement consisting of an inversion that results in translational skipping of exon 10 in the APC gene.
Heat treatment improves antigen-specific T cell activation after protein delivery by several but not all yeast genera.
Saarbrücken, Germany. In Vaccine, 24 Apr 2014
Here, we investigated the distribution of major yeast cell wall components such as mannan, β-glucan and chitin of four different and likewise biotechnologically relevant yeasts (Saccharomyces, Pichia, Kluyveromyces and Schizosaccharomyces) and analyzed the influence of heat-treatment on β-1,3-glucan exposure at the outer yeast cell surface as well as the amount of yeast induced reactive oxygen species (ROS) production by antigen presenting cells (APC) in human blood.
Telomeres, telomerase and colorectal cancer.
Padova, Italy. In World J Gastroenterol, 28 Mar 2014
The erosion of telomeres, mainly because of cell proliferation, may be accelerated by specific alterations in the genes involved in CRC, such as APC and MSH2.
Detection of an activated JAK3 variant and a Xq26.3 microdeletion causing loss of PHF6 and miR-424 expression in myelodysplastic syndromes by combined targeted next generation sequencing and SNP array analysis.
Gießen, Germany. In Pathol Res Pract, 23 Mar 2014
In addition to the detection of mutations known to be associated with MDS in NRAS, KRAS, MPL, NPM1, IDH1, PTPN11, APC and MET, single nucleotide variants so far unrelated to MDS in STK11 (n=1), KDR (n=3), ATM (n=1) and JAK3 (n=2) were identified.
Is cancer vaccination feasible at older age?
Ben Mehidi, Algeria. In Exp Gerontol, 05 Mar 2014
Major immune defects at older age are lack of naïve T cells, impaired activation pathways of T cells and antigen-presenting cells (APC), and age-related changes in the tumor microenvironment (TME).
Visualizing how T cells collect activation signals in vivo.
Paris, France. In Curr Opin Immunol, 28 Feb 2014
Additionally, the development of more sophisticated fluorescent tools has permitted not only to visualize T cell-APC contacts but also to probe their functional impact on T cell activation.
Kinetic framework of spindle assembly checkpoint signalling.
More papers using
Vienna, Austria. In Nat Cell Biol, Nov 2013
We identify two mechanisms by which cells can bypass the SAC: first, single unattached chromosomes inhibit the anaphase-promoting complex/cyclosome (APC/C) less efficiently than a full complement of unattached chromosomes; second, because of the relatively slow kinetics of re-imposing APC/C inhibition during metaphase, cells were unresponsive to chromosome detachment up to several minutes before anaphase onset.
siRNA and innate immunity
In Molecular Therapy. Nucleic Acids, 2008
Then, cells were washed with phosphate-buffered saline and analyzed in the APC channel with a fluorescence-activated cell sorting canto II (BD Biosciences, Franklin Lakes, NJ) ... CD8+ T cells in autoimmunity
In Indian Journal of Human Genetics, 2004
... antibodies: Anti-human GITR FITC mAb, anti-human CD8 PE mAb, purified phopspho-specific JNK mAb, goat anti-mouse APC mAb and purified anti-human CD3mAb were purchased from BD Biosciences (San Jose, CA, USA) ...