UNASSIGNED: Here, we reported a strategy based plasmonic enzyme-linked immunosorbent assay (ELISA) using alcohol dehydrogenase-catalyzed gold nanoparticle seed-mediated growth to serve as a colorimetric signal generation method for detecting disease biomarkers with the naked eye.
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Elimination of alcohol dehydrogenase E (adhE), acetate kinase A-phosphotransacetylase (ackA-pta), and lactate dehydrogenase A (ldhA) enzymes allowed BDO production as a primary pathway for NADH re-oxidation, and significantly reduced by-products.
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When two to six copies of a white promoter-Alcohol dehydrogenase (Adh) reporter fusion gene are introduced into the genome, the expression is progressively reduced both in larvae and adults rather than the expected gene dosage effect.