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V-akt murine thymoma viral oncogene homolog 1

Akt, Rac, PKB, protein kinase B
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011] (from NCBI)
Top mentioned proteins: PI3K, V1a, CAN, mTOR, ERK
Papers using Akt antibodies
CCL2 increases MMP-9 expression and cell motility in human chondrosarcoma cells via the Ras/Raf/MEK/ERK/NF-κB signaling pathway
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Hsiao Li-Der et al., In Journal of Neuroinflammation, 2011
... Anti-phospho-PDGFR, anti-phospho-c-Src, anti-phospho-p42/p44 MAPK, anti-phospho-p38 MAPK, anti-phospho-JNK1/2, and anti-phospho-Akt antibodies were from Cell Signaling (Danver, MA) ...
Slug is upregulated during wound healing and regulates cellular phenotypes in corneal epithelial cells.
Supplier
Srivastava Rakesh K., In PLoS ONE, 2011
... anti-Ki-67, anti-GSK-3β antibody, anti-EGFR antibody, anti-GAPDH antibody, anti-p-GSK-3β antibody, anti-ERK1/2 antibody anti-p-ERK1/2 antibody, anti-AKT antibody, anti-p-AKT antibody were from Cell Signaling Technology; anti-vimentin antibody was ...
Mitigating endoplasmic reticulum stress with revaprazan ameliorates stress-related mucosal disease
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Hahm Ki-Baik et al., In Journal of Clinical Biochemistry and Nutrition, 2011
... Rabbit polyclonal Akt and pAkt antibodies were obtained from Cell signaling Technology Inc ...
Preliminary efficacy of the anti-insulin-like growth factor type 1 receptor antibody figitumumab in patients with refractory Ewing sarcoma
Supplier
Jürgens Heribert et al., In Sarcoma, 2010
... -IR3, doxorubicin, z-VAD-fmk, z-DEVD-fmk, wortmannin, AKTi-1/2 (AKT inhibitor VIII; Merck Chemicals, Darmstadt, Germany), fumonisin ...
CCR9-CCL25 interactions promote cisplatin resistance in breast cancer cell through Akt activation in a PI3K-dependent and FAK-independent fashion.
Supplier
Maki Carl G., In PLoS ONE, 2010
... Monoclonal antibody Phospho-Akt (Ser473) and Phospho-Akt (Thr308) were purchased from Cell Signaling (Danvers, MA) ...
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Papers on Akt
Protein tyrosine phosphatase-σ regulates hematopoietic stem cell-repopulating capacity.
New
Chute et al., In J Clin Invest, 21 Dec 2014
Functionally, PTPσ-deficient BM cells displayed increased cobblestone area-forming cell (CAFC) capacity and augmented transendothelial migration capacity, which was abrogated by RAC inhibition.
The Anticancer/Antiviral Agent Akt Inhibitor-IV Massively Accumulates in Mitochondria and Potently Disrupts Cellular Bioenergetics.
New
Peterson et al., In Acs Chem Biol, 21 Dec 2014
UNLABELLED: Inhibitors of the PI3-kinase / Akt (protein kinase B) pathway are under investigation as anticancer and antiviral agents.
Biomarker Analyses in CLEOPATRA: A Phase III, Placebo-Controlled Study of Pertuzumab in Human Epidermal Growth Factor Receptor 2-Positive, First-Line Metastatic Breast Cancer.
New
Impact
Swain et al., Barcelona, Spain. In J Clin Oncol, 20 Dec 2014
PATIENTS AND METHODS: Mandatory tumor and serum samples were collected (N = 808; 58% to 99.8% were assessable), and amphiregulin, betacellulin, epidermal growth factor (EGF), transforming growth factor alpha, EGF receptor, HER2, HER3, insulin-like growth factor 1 receptor, PTEN, phosphorylated AKT, PIK3CA, CMYC, serum HER2 extracellular domain (sHER2), and FCγR were assessed using appropriate assays.
Insulin signaling: implications for podocyte biology in diabetic kidney disease.
New
Fornoni et al., Miami, United States. In Curr Opin Nephrol Hypertens, 19 Dec 2014
RECENT FINDINGS: Akt2, the major isoform of Akt activated following cellular insulin stimulation, protects against the progression of renal disease in nephron-deficient mice, and podocyte-specific deletion of Akt2 results in a more rapid progression of experimental glomerular disease.
Recent studies of 5-fluorouracil resistance in pancreatic cancer.
Review
New
Shu et al., Beijing, China. In World J Gastroenterol, 14 Dec 2014
Intracellular signaling pathways are an integrated network, and nuclear factor kappa-light-chain-enhancer of activated B cells, AKT and extracellular signal-regulated kinases are signaling pathways that are particularly relevant to 5-FU resistance.
Phospholipase D signaling pathways and phosphatidic acid as therapeutic targets in cancer.
Review
New
Impact
Brown et al., Nashville, United States. In Pharmacol Rev, 31 Oct 2014
Many of these cellular events are modulated by the actions of phosphatidic acid, and identification of two targets (mammalian target of rapamycin and Akt kinase) has especially highlighted a role for phospholipase D in the regulation of cellular metabolism.
Loss of signalling via Gα13 in germinal centre B-cell-derived lymphoma.
New
Impact
Cyster et al., San Francisco, United States. In Nature, 28 Oct 2014
Here we show, using in vitro and in vivo assays, that GCB-DLBCL-associated mutations occurring in S1PR2 frequently disrupt the receptor's Akt and migration inhibitory functions.
mTOR- and HIF-1α-mediated aerobic glycolysis as metabolic basis for trained immunity.
New
Impact
Netea et al., Nijmegen, Netherlands. In Science, 26 Oct 2014
Trained monocytes display high glucose consumption, high lactate production, and a high ratio of nicotinamide adenine dinucleotide (NAD(+)) to its reduced form (NADH), reflecting a shift in metabolism with an increase in glycolysis dependent on the activation of mammalian target of rapamycin (mTOR) through a dectin-1-Akt-HIF-1α (hypoxia-inducible factor-1α) pathway.
Intraovarian control of early folliculogenesis.
New
Impact
Fauser et al., Stanford, United States. In Endocr Rev, 09 Oct 2014
Although the exact hormonal factors involved in primordial follicle activation has yet to be elucidated, the AKT and mTOR signaling pathways are important for the activation of dormant primordial follicles.
Hepatitis B virus X protein accelerates the development of hepatoma.
Review
New
Ye et al., Tianjin, China. In Cancer Biol Med, Sep 2014
HBx interacts with various signal-transduction pathways, such as protein kinase B/Akt, Wnt/β-catenin, signal transducer and activator of transcription, and NF-κB pathways.
Chemoquiescence for ideal cancer treatment and prevention: where are we now?
Review
New
Hahm et al., Seoul, South Korea. In J Cancer Prev, Jun 2014
Recently potential applications of metformin or chloroquine as well as the potential drugs under investigation such as proton pump inhibitor, sonic hedgehog inhibitor, and Akt inhibitor, are actively investigated in this field of chemoquiescence to achieve cancer cure far beyond those of chemoprevention.
Post-Injury Treatment with 7,8-Dihydroxyflavone, a TrkB Receptor Agonist, Protects against Experimental Traumatic Brain Injury via PI3K/Akt Signaling.
New
Chen et al., Taipei, Taiwan. In Plos One, Dec 2013
7,8-DHF enhanced phosphorylation of TrkB, Akt (Ser473/Thr308), and Bad at day 4, but had no effect on Erk 1/2 phosphorylation.
Therapeutic Targeting the Cell Division Cycle 25 (CDC25) Phosphatases in Human Acute Myeloid Leukemia - The Possibility to Target Several Kinases through Inhibition of the Various CDC25 Isoforms.
Review
New
Bruserud et al., Bergen, Norway. In Molecules, Dec 2013
Firstly, upregulation of CDC25A can enhance cell proliferation and the expression seems to be controlled through PI3K-Akt-mTOR signaling, a pathway possibly mediating chemoresistance in human AML.
Rho family GTPase-dependent immunity in plants and animals.
Review
New
Shimamoto et al., Ikoma, Japan. In Front Plant Sci, Dec 2013
Accumulating evidence indicates that Rac (also known as Rop), a member of the Rho family of small GTPases, is a key regulator of immunity in plants and animals.
Akt Inhibition Promotes ABCA1-Mediated Cholesterol Efflux to ApoA-I through Suppressing mTORC1.
New
Zha et al., Ottawa, Canada. In Plos One, Dec 2013
We report here that one of major signaling pathways in mammalian cells, Akt, is also involved.
MiR-26a enhances metastasis potential of lung cancer cells via AKT pathway by targeting PTEN.
GeneRIF
Xu et al., Tianjin, China. In Biochim Biophys Acta, 2012
Our study demonstrated that miR-26a enhanced lung cancer cell metastasis potential via modulation of metastasis-related gene expression, and activation of AKT pathway by PTEN suppression
The Akt-SRPK-SR axis constitutes a major pathway in transducing EGF signaling to regulate alternative splicing in the nucleus.
GeneRIF
Fu et al., San Diego, United States. In Mol Cell, 2012
Blocking individual branches in epidermal growth factor (EGF) signaling leads to Akt activation and plays a major role in EGF- induced alternative splicing.
Diverse regulation of AKT and GSK-3β by O-GlcNAcylation in various types of cells.
GeneRIF
Gong et al., Taiwan. In Febs Lett, 2012
analysis of regulation of AKT and GSK-3beta by O-GlcNAcylation in various types of cells
Selective effects of PD-1 on Akt and Ras pathways regulate molecular components of the cell cycle and inhibit T cell proliferation.
GeneRIF
Boussiotis et al., Boston, United States. In Sci Signal, 2012
Selective effects of PD-1 on Akt and Ras pathways regulate molecular components of the cell cycle and inhibit T cell proliferation.
Sarcospan-dependent Akt activation is required for utrophin expression and muscle regeneration.
GeneRIF
Crosbie-Watson et al., Los Angeles, United States. In J Cell Biol, 2012
SSPN-null mice displayed delayed differentiation after cardiotoxin injury caused by loss of utrophin and Akt signaling
More papers using Akt antibodies
Sustained delivery of sphingosine-1phosphate using poly(lactic-co-glycolic acid)-based microparticles stimulates Akt/ERK-eNOS mediated angiogenesis and vascular maturation restoring blood flow in ischemic limbs of mice
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Sunagawa Kenji et al., In Clinical Science (London, England : 1979), 2009
... Antibodies against ERK (extracellular-signal-regulated kinase), p38 MAPK (mitogen-activated protein kinase), JNK (c-Jun N-terminal kinase) and Akt and their phosphorylated (p) forms were obtained from Cell Signaling Technology ...
The PKB/AKT pathway in cancer.
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Aiyar Ashok, In PLoS ONE, 2009
... NCL-c-erbB-3); HER4 (clone sc-283), 1∶500 (Santa Cruz, ErbB-4); Akt1 (clone 2H10), 1∶500 (Cell Signaling, 2967); phospho-Akt (pAkt, Ser473), 1∶75 (Cell Signaling, 4051); PTEN (clone 28H6), ...
Lactate dehydrogenase 5 expression in melanoma increases with disease progression and is associated with expression of Bcl-XL and Mcl-1, but not Bcl-2 proteins
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Zhang X D et al., In Oncogene, 2009
... The rabbit antibodies against Akt, phospho-Akt (Ser473) were from Cell Signaling Technology (Danvers, MA, USA) ...
Akt cys-310-targeted inhibition by hydroxylated benzene derivatives is tightly linked to their immunosuppressive effects
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Cho Jae Youl et al., In Mediators of Inflammation, 2009
... Phospho-specific antibodies to p85, PDK1, Akt, and IκBα  were obtained from Cell Signaling (Beverly, MA) ...
Targeted therapies in epithelial ovarian cancer
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Bauer Joseph Alan, In PLoS ONE, 2009
... Antibodies against Cl-caspase3, Cl-PARP, p-EGFR (Tyr-1068), EGFR, p-AKT (Ser-473), p- mTOR (Ser 2481), Raptor and AKT antibodies were obtained from Cell Signaling Technology (Danvers, MA) ...
Chronic hyperglycemia reduces substrate oxidation and impairs metabolic switching of human myotubes.
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Moran Maria, In PLoS ONE, 2009
... Phospho-Akt (Ser473) and Akt antibodies were from Cell Signaling Technology (Beverly, MA), OXPHOS ...
The hinge region of the human estrogen receptor determines functional synergy between AF-1 and AF-2 in the quantitative response to estradiol and tamoxifen.
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Korach Kenneth S. et al., In Environmental Health Perspectives, 2009
... MAPK antibody (9102), phospho-GSK-3β antibody (9323), total GSK-3β antibody (9315), phospho-Akt antibody (4060), and total Akt antibody (9272) from Cell Signaling Technology (Danvers, MA).
Molecular mechanisms of resistance to tumour anti-angiogenic strategies
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Fotsis Theodore et al., In Molecular Cancer, 2009
... Rabbit polyclonal anti-phospho-p38, anti-ERK1/2, anti-phospho-ERK1/2, anti-phospho-Akt and anti-Akt antibodies were obtained from Cell Signaling (Cell Signaling Technology, Inc, ...
High-throughput assay for modulators of mitochondrial membrane potential identifies a novel compound with beneficial effects on db/db mice
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Xu Aimin, In PLoS ONE, 2009
... antibodies against Acetyl-CoA Carboxylase, Phospho-Acetyl-CoA Carboxylase (Ser79), AMPKα, Phospho-AMPKα (Thr172), Phospho-p38 MAP Kinase (Thr180/Tyr182), Akt, Phospho-Akt (Ser473) were purchased from Cell Signaling Technology (Danvers, MA, USA) ...
The role of caveolin-1 in prostate cancer: clinical implications
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Hong Wanjin, In PLoS ONE, 2009
... Caveolin-1 and Rac-1 antibodies were from BD Biosciences.
Dependence on phosphoinositide 3-kinase and RAS-RAF pathways drive the activity of RAF265, a novel RAF/VEGFR2 inhibitor, and RAD001 (Everolimus) in combination.
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Bernhard Eric J., In PLoS ONE, 2009
... Antibodies against total and phosphorylated ERK and AKT were purchased from Cell Signaling Technology (Danvers, MA) ...
Overall survival analysis of a phase II randomized controlled trial of a Poxviral-based PSA-targeted immunotherapy in metastatic castration-resistant prostate cancer
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Chen Wei et al., In Breast Cancer Research : BCR, 2009
... HER2 (29D8), HER3, Akt, pAkt, and surviving antibodies were from Cell Signaling (Beverly, MA, USA) ...
EML4-ALK mutations in lung cancer that confer resistance to ALK inhibitors
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Gaits-Iacovoni F et al., In Blood Cancer Journal, 2009
... NY, USA), phospho-ALK-Y1604 (recognizes Y664 on NPM–ALK), phospho-p38-T180/Y182, p38, phospho-Erk1/2- T202/Y204, Erk1/2, phospho-STAT3-Y705, STAT3, phospho-Akt-S473, Akt were all from Cell Signaling Technologies (Beverly, MA, USA) ...
FRET and mechanobiology.
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Cordes Nils, In PLoS ONE, 2008
... A specific inhibitor of Rac GTPase, N6-[2-[[4-(Diethylamino)-1-methylbutyl]amino]-6-methyl-4-pyrimidinyl]-2-methyl-4,6-quinolinediamine trihydrochloride (NSC23766), from Tocris Bioscience (Ellisville, Missouri), was ...
Cell and molecular mechanisms of keratinocyte function stimulated by insulin during wound healing.
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Brandner Johanna M., In PLoS ONE, 2008
... Anti-phospho-AKT (serine 473) antibody was from Cell Signaling Technology (Beverly, MA, USA) ...
Microtubules and cadherins: a neglected partnership.
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Ushio-Fukai Masuko, In PLoS ONE, 2008
... The following antibodies were used for western blotting : rabbit anti-Akt (1∶1000; Cell Signaling Technology, Beverly, MA), rabbit ...
Bone marrow microenvironment and the identification of new targets for myeloma therapy.
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Shi Songtao, In PLoS ONE, 2008
... Rabbit Abs to NF-κB p65, phospho-ERK1/2 (Thr202/Tyr204), phospho-AKT (Ser473), phosphor-IκBα (Ser32), ERK, AKT, PARP, horseradish peroxidase-conjugated anti-rabbit IgG and horseradish peroxidase-conjugated anti-mouse IgG were purchased from Cell Signaling Technology ...
Systems biology of growth factor-induced receptor endocytosis
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Polo Simona et al., In Molecular Systems Biology, 2008
... (clone C-20, Santa Cruz), polyclonal anti-SLC3A2 (H-300, Santa Cruz), monoclonal anti-Cbl (BD), anti-phospho-ERK (Cell Signaling), anti-phospho-AKT (Cell Signaling) and anti-pY (Clone 4G10, ...
A clathrin-dependent pathway leads to KRas signaling on late endosomes en route to lysosomes
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Pal Soumitro, In PLoS ONE, 2008
... CA), anti-Ras (Santa Cruz Biotechnology, Santa Cruz, CA), anti-Rab5 (Santa Cruz Biotechnology, Santa Cruz, CA), anti-AKT (Cell Signaling Technology, Danvers, MA), anti-AKT-p473 ...
A phosphorylation-dependent intramolecular interaction regulates the membrane association and activity of the tumor suppressor PTEN.
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Hotchin Neil A., In PLoS ONE, 2008
... monoclonal c-myc (9E10; WB 1∶1,000; IF 1∶500; immunoprecipitation [IP] 1∶200; Abcam, Cambridge, UK), rabbit polyclonal phospho-Akt (serine 473; #9271; WB 1∶1,000; Cell Signaling Technology, Danvers, MA, USA), ...
Effect of gefitinib on N-nitrosamine-4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone induced lung tumorigenesis in A/J mice.
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Sun Shi-Yong, In PLoS ONE, 2008
... Santa Cruz), anti-β-Tubulin and -β-Actin (Sigma-Aldrich); and anti-phospho-EGFR (Y1086, Abcam), and anti-EGFR, -ERK, -Akt and -phospho-Akt (Ser473) (Cell Signaling Technology); anti-phospho-ERK (Y185/187) (Biosource) ...
Active-site inhibitors of mTOR target rapamycin-resistant outputs of mTORC1 and mTORC2.
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Bozza Patricia T., In PLoS ONE, 2008
... AMPKα, phospho-AMPKα, acetyl CoA carboxylase, phospho-acetyl CoA carboxylase, raptor, phospho-eIF2α, acetylated lysine, acetylated-p53, AKT, and phospho-AKT were purchased from Cell Signaling Technology (Danvers, MA, USA.) ...
Targeting PI3K signalling in cancer: opportunities, challenges and limitations.
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Ahmad Aamir, In PLoS ONE, 2008
... Antibodies against p-mTOR (Ser2448), p-p70S6K (Thr421/Ser424), p-eIF4E (Ser209), p-4EBP1 (Thr37/46), p-GSK-3β (Ser9) and Akt were purchased from Cell Signaling Technology ...
Discovery of a novel proteasome inhibitor selective for cancer cells over non-transformed cells
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Sebti Saïd M. et al., In Cell death and differentiation, 2008
... Antibodies were from the following sources: Akt, pAkt(T308), pAkt(S473) and pGSK3-β(S9) (Cell Signaling, Danvers, MA), mTOR (Millipore, ...
An unusual insertion in Jak2 is crucial for kinase activity and differentially affects cytokine responses
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Lenting Peter J., In PLoS ONE, 2008
... The anti-JAK2, anti-phospho-S6 (pS235/236), anti-S6, anti-phospho-Akt (pS473), anti-Akt antibodies were purchased from Cell Signaling (Beverly, MA) ...
Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy.
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Migliaccio Antimo, In PLoS ONE, 2008
... mouse anti phospho-Erk1/2 (Sigma, 8159), polyclonal rabbit anti JNK (Cell Signaling, 9252), polyclonal rabbit anti phospho-Ser473-Akt (Cell Signaling, 9271), polyclonal rabbit anti ...
miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells.
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Afford Simon, In PLoS ONE, 2007
... -Akt antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA) ...
Involvement of matrix metalloproteinase-9 in stromal cell-derived factor-1/CXCR4 pathway of lung cancer metastasis
Supplier
Hung Chien-Hui et al., In Carcinogenesis, 2007
... p-IκBα, IKKα/β, p65, p50, p-ERK, p-p38, p-JNK, p-Akt, ERK, p38, c-Jun N-terminal kinase (JNK) and Akt were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) ...
Influence of tooth removal on mandibular bone response to mastication
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Bloch Wilhelm et al., In Journal of Biomedicine and Biotechnology, 2007
... Mouse antiphospho-Akt1/PKBα (Ser473) monoclonal antibody was obtained from Cell Signaling Technology (Beverly, MA, USA) ...
DNMT1 as a molecular target in a multimodality-resistant phenotype in tumor cells
Supplier
Berghe Wim Vanden et al., In Molecular Cancer, 2007
... The phospho-specific antibodies directed against p65 Ser536, p38 and p44/42 MAPK, cjun, Akt, MEK were from Cell Signaling (Beverly, CA) ...
Paraquat neurotoxicity is mediated by a Bak-dependent mechanism
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O'Donnell Janis et al., In Parkinson's Disease, 2007
The transgenic strain ...
Membrane phosphatidylserine regulates surface charge and protein localization
Supplier
Grinstein Sergio et al., In The Journal of Cell Biology, 2007
... Mouse monoclonal anti-phosphotyrosine (4G10) and anti-GAPDH antibodies were from Millipore, rabbit monoclonal anti-phospho-Akt (Ser 473) antibody was from Cell Signaling Technology, mouse monoclonal anti-Rac1 ...
Interaction of p21(CDKN1A) with PCNA regulates the histone acetyltransferase activity of p300 in nucleotide excision repair.
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Imperiale Michael, In PLoS Pathogens, 2007
... AKT inhibitor LY294002 was purchased from Cell Signaling Technology and used at ...
Microarray analysis of human monocytes infected with Francisella tularensis identifies new targets of host response subversion.
Supplier
Rudd Christopher E., In PLoS ONE, 2007
... Actin and Akt antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) ...
Inactivation of Adenosine A2A Receptor Attenuates Basal and Angiotensin II-induced ROS Production by Nox2 in Endothelial Cells*
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Li Jian-Mei et al., In The Journal of Biological Chemistry, 2007
... Antibodies to phospho-ERK1/2, phospho-p38 MAPK, phospho-JNK, and phospho-Akt were from Cell Signaling Technology ...
Class II Phosphoinositide 3-Kinase Regulates Exocytosis of Insulin Granules in Pancreatic β Cells*
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Maffucci Tania et al., In The Journal of Biological Chemistry, 2007
... and anti-SNAP25 (1:1000) were from Santa Cruz Biotechnology (Santa Cruz, CA), anti-phosphotyrosine (1:1000) and anti-phospho-Ser-473 Akt (1:500) were from Cell Signaling Technology (Danvers, MA), anti-p110γ ...
Comprehensive genomic characterization defines human glioblastoma genes and core pathways.
Supplier
Lesniak Maciej, In PLoS ONE, 2007
... (4058), and AKT (9272) were purchased from Cell Signaling; goat and mouse EphA2 ...
Class 1A PI3K regulates vessel integrity during development and tumorigenesis
Supplier
Ridley Anne J. et al., In The Journal of Cell Biology, 2007
... from BD; RhoA, -B, and -C and Tiam-1 were purchased from Santa Cruz Biotechnology, Inc.; Akt, pAktS473, pAktT308, Pyk2, and Pyk2Y402 were obtained from Cell Signaling Technology; VE-cadherin–Y658, glyceraldehyde 3-phosphate ...
Targeting phosphoinositide 3-kinase: moving towards therapy.
Supplier
Bratton Shawn B., In PLoS ONE, 2007
... p110α, p110β, and phospho-specific Akt antibodies were from Cell Signaling Technology ...
A novel calcium-binding protein is associated with tau proteins in tauopathy.
Supplier
Sanchez-Margalet Victor, In PLoS ONE, 2007
... Rabbit mAb against Akt and phosphorylated Akt at Ser473 (pAkt) were obtained from Cell Signaling Technology (Beverly, MA, USA) ...
Unrestrictive identification of multiple post-translational modifications from tandem mass spectrometry using an error-tolerant algorithm based on an extended sequence tag approach
Supplier
Srinivasula Srinivasa M., In PLoS ONE, 2007
... anti-PARP and anti-JNK and anti-phospho-JNK (Thr183/Tyr185) from Santa Cruz Biotechnology (CA, USA), and anti-Akt and anti-phospho-Akt (Ser473) from Cell Signaling Technology (MA, USA); the ...
Enhanced recovery from contraction-induced damage in skeletal muscles of old mice following treatment with the heat shock protein inducer 17-(allylamino)-17-demethoxygeldanamycin
Supplier
Chang Jang-Yang et al., In Molecular Cancer, 2007
... antibody (Santa Cruz Biotechnology, Santa Cruz, CA), a rabbit anti-Survivin antibody (R&D Systems, Minneapolis, MN), a rabbit anti-Akt antibody (Cell Signaling Technology, Danvers, MA) and ...
Evidence that MIG-6 is a tumor-suppressor gene
Supplier
Segatto Oreste et al., In The Journal of Cell Biology, 2006
... Anti-pEGFR antibodies, anti-pERK, anti-pAKT, anti-ERK, and anti-AKT were from Cell Signaling Technology ...
Induction of Tumorigenesis and Metastasis by the Murine Orthologue of Tumor Protein.
Supplier
Bauer Joseph Alan, In PLoS ONE, 2006
... site-specific polyclonal antibodies for ERK1 (pThr202 and pTyr204), ERK2 (pThr185 and pTyr187) MAP kinases, and Akt (pSer473 and pThr308) were from Cell Signaling Technology (Beverly, MA) ...
The glucose transporter 4-regulating protein TUG is essential for highly insulin-responsive glucose uptake in 3T3-L1 adipocytes
Supplier
Toomre Derek K. et al., In The Journal of Cell Biology, 2006
... Antibodies used were: mouse anti-myc (9E10; Covance), anti-Akt and phospho-Akt antibodies (Cell Signaling Technology), anti–β-actin (Abcam), anti-VAMP2 ...
Epithelial--mesenchymal and mesenchymal--epithelial transitions in carcinoma progression
Supplier
Lee Ming-Ting et al., In Molecular Cancer, 2006
... Anti-Akt was obtained from Cell Signaling (Boston, MA) ...
Ligand density dramatically affects integrin alpha IIb beta 3-mediated platelet signaling and spreading.
Supplier
Gullberg Donald, In PLoS ONE, 2006
... Anti-Cdc42 antibody was purchased from BD Biosciences and anti-PAK and p-PAK1/2, Akt and p-Akt antibodies were obtained from Cell Signaling.
mVps34 is activated by an acute bout of resistance exercise
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Baar Keith et al., In BMC Cell Biology, 2006
... , t-p44/42, and t-PKB were from Cell Signaling Technologies and were used ...
Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo
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Bruni Paola et al., In Biochimie, 2006
... of retinoblastoma gene (pRb) (Ser 795), phosphorylated glycogen synthase kinase-3β (GSK-3β) (Ser 9), pan-Akt (H-136), phospho-pan-Akt (Ser 473), anti-mouse, anti-rabbit, and anti-goat immunoglobulin G1 conjugated to horseradish peroxidase and Blotto (nonfat dry milk) were obtained from Santa Cruz Biotechnology, Inc ...
Cell polarity in development and cancer.
Supplier
Thi Thu Nguyen Hang, In PLoS ONE, 2006
... The antibodies for detection of phospho-AKT (Ser473) and AKT were from Cell Signaling Technology (Danvers, MA) ...
Using GOstats to test gene lists for GO term association.
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Valcárcel Juan et al., In Genome Biology, 2006
... denaturing polyacrylamide gels, transferred to nitrocellulose membrane (Schleicher and Schuell, Dassel Germany) and probed with anti-phospho-Akt (Ser473) antibody (1:1000; Cell Signaling, Boston, MA, USA) or ...
Platelet-derived nitric oxide signaling and regulation
Supplier
Lin Kuan-Hung et al., In Evidence-based Complementary and Alternative Medicine : eCAM, 2006
... ) and anti-Akt mAbs were from Biovision (Mountain View, CA, USA) ...
Osteoclast differentiation and gene regulation
Supplier
Kim Jeong-Joong et al., In Anatomy & Cell Biology, 2006
... Antibodies against Phospho (p)-p38, p-JNK, p-ERK, p-Akt, Akt, I-κB was purchased from Cell signaling Technology (Beverly, MA) ...
Genetic ablation of Rac1 in cartilage results in chondrodysplasia
Supplier
Beier Frank et al., In BMC Developmental Biology, 2006
... (P-Akt (Ser 473) #4051; Akt #9272), Santa Cruz Biotechnology ...
Pterostilbene induces apoptosis and cell cycle arrest in human gastric carcinoma cells
Supplier
Chen Wei-Jen et al., In Evidence-based Complementary and Alternative Medicine : eCAM, 2006
... Antibodies against phospho-p38 (Thr180/Tyr182), p38, phospho-ERK1/2 (Thr42/Tyr44), ERK1/2, phospho-Akt (Ser473) and Akt were from Cell Signaling Technology (Beverly, MA, USA) ...
IGFBP-5 regulates muscle cell differentiation by binding to IGF-II and switching on the IGF-II auto-regulation loop
Supplier
Duan Cunming et al., In The Journal of Cell Biology, 2005
... and Myogenin (M-225) were purchased from Santa Cruz Biotechnology, Inc., and antibodies to Akt and phospho-Akt (Ser473) were from Cell Signaling Technology ...
Necrotic death as a cell fate.
Supplier
Wu Gen Sheng, In PLoS ONE, 2005
... Antibodies against p-ERK, FOXO3a, p-FOXO3a (Thr32), p-FOXO3a (Ser253), Bad, PARP, NOXA and PUMA, and the AKT kinase assay kit were purchased from Cell Signaling Technology (Danvers, MA) ...
uPAR induces epithelial–mesenchymal transition in hypoxic breast cancer cells
Supplier
Gonias Steven L. et al., In The Journal of Cell Biology, 2005
... Antibodies that detect phosphorylated ERK/MAPK, phosphorylated Akt, and phosphorylated GSK-3β were obtained from Cell Signaling Technologies ...
A death-promoting role for extracellular signal-regulated kinase
Supplier
Yaffe Michael B et al., In Molecular Systems Biology, 2005
... Antibodies detecting phosphorylated forms of H2AX (Ser-139), p38 MAPK (Thr-180/Tyr-182), JNK (Thr-183/Tyr-185), ERK1/2 (Thr-202/Tyr-204), and Akt (Ser-473) were obtained from Cell Signaling Technologies ...
A proteomic screen identified stress-induced chaperone proteins as targets of Akt phosphorylation in mesangial cells.
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Cheriyath Venugopalan, In PLoS ONE, 2005
... anti-phospho-mTOR (Ser2448), anti-phospho-HDM2 (Ser166), anti-phospho-FOXO1 (Ser319), anti-phospho-GSK3α/β (Ser21/9), immobilized AKT (1G1) antibodies (bead conjugated), immobilized phospho-AKT (Ser473) (D9E) antibodies (bead conjugated) and immobilized IgG mouse (bead conjugated) were from Cell Signaling Technology (New England Biolabs ...
Cancer vaccines targeting carbohydrate antigens
Supplier
Iwamori Masao et al., In Journal of Experimental & Clinical Cancer Research : CR, 2005
... monoclonal antibody, PCNA and β-actin from Santa Cruz Biotechnology (Santa Cruz, CA, USA); Akt and p-Akt from Cell Signaling Technology, Inc ...
Butylated hydroxyanisole regulates ARE-mediated gene expression via Nrf2 coupled with ERK and JNK signaling pathway in HepG2 cells
Supplier
Yang Chuen-Mao et al., In Journal of Neuroinflammation, 2005
... Anti-phospho-PDGFR and anti-phospho-Akt antibodies were purchased from Cell Signaling (Danver, MA) ...
Resveratrol inhibits insulin responses in a SirT1-independent pathway.
Supplier
Kaeberlein Matt, In PLoS ONE, 2005
... Antibodies against Phospho Akt (ser473, CST) Akt (CST), Hsp72 (Abcam), P-Gsk3β (Abcam), Erk1/2 (Sigma), ...
(-)-Epigallocatechin gallate overcomes resistance to etoposide-induced cell death by targeting the molecular chaperone glucose-regulated protein 78.
Supplier
Pizzo Salvatore V., In PLoS ONE, 2005
... The Akt kinase assay kit was acquired from Cell Signaling Technology (Boston, MA) ...
Tumor necrosis factor inhibitors for rheumatoid arthritis
Supplier
Roodman G David et al., In Journal of Bone and Mineral Research, 2005
... Antibodies to phosphorylated and total Pyk2, Src, paxillin, Cbl, Erk1/2, JNK, p38 MAPK, Akt, and IκBα were from Cell Signaling Technology (Danvers, MA, USA), ...
Intrahepatic cytokine expression is downregulated during HCV/HIV co-infection.
Supplier
Zheng Jialin Charles, In PLoS ONE, 2005
... Phosphorylation-specific polyclonal antibodies against extracellular signal-regulated kinase (ERK) and Akt (Ser 473) were purchased from Cell Signaling Technology (Beverly, Massachusetts, USA) ...
Differentiating pancreatic lesions by microarray and QPCR analysis of pancreatic juice RNAs.
Supplier
Vanacker Jean-Marc, In PLoS ONE, 2005
... PD98059 (#9900), LY294002 (#9901), MAPK Family Antibody Sampler Kit (#9926), Phospho-Akt (Ser473), Antibody (#9271) and SDF-1 antibody (#3530) were purchased from Cell Signaling Technology (USA) ...
Increased infiltration of macrophages in omental adipose tissue is associated with marked hepatic lesions in morbid human obesity
Supplier
Greenberg Andrew S. et al., In Diabetes, 2005
... Antibodies recognizing ERK (9102), phospho-ERK (9101), JNK (9252), phospho-JNK (9251), AKT (9272), and phospho-Akt (9271) were obtained from Cell Signaling (Danvers, MA), and a MAC-2 ...
Antioxidant activity of caffeoyl quinic acid derivatives from the roots of Dipsacus asper Wall
Supplier
Wei Bai-Luh et al., In Evidence-based Complementary and Alternative Medicine : eCAM, 2005
... against total (i.e., inactivated) and the phosphorylated (i.e., activated) forms of p38, ERK1/2, JNK and Akt were purchased from Cell Signaling Technology (Beverly, MA, USA) ...
Regulation of angiogenesis and tumor growth by p110 alpha and AKT1 via VEGF expression.
Supplier
Navarro Alfons, In PLoS ONE, 2005
... Antibodies against PTEN, phospho-ERK1/2, phospho-AKT (Ser-473), and total AKT were products of Cell Signaling Technology (Beverly, MA) ...
De novo emergence of insulin-stimulated glucose uptake in human aortic endothelial cells incubated with high glucose
Supplier
Salt Ian P. et al., In Biochemical and Biophysical Research Communications, 2005
... alanine-rich protein kinase C substrate (MARCKS) (Ser152/156), anti-phospho-extracellular signal regulated kinase (ERK)1/2 (Thr202/Tyr204); anti-ERK1/2; anti-Akt and anti-phospho-Akt (Ser473) antibodies were from New England Biolabs UK (Hitchin, UK) ...
Serum resistance-associated protein blocks lysosomal targeting of trypanosome lytic factor in Trypanosoma brucei.
Supplier
Blagosklonny Mikhail, In PLoS ONE, 2005
... Anti-pErk1/2, anti-Erk1/2, anti-pAkt (serin 473), and anti-Akt antibodies were purchased from Cell Signaling Technology, Danvers, MA ...
An inhibitor of Bcl-2 family proteins induces regression of solid tumours
Supplier
Bondada Subbarao et al., In Molecular Cancer, 2004
... Antibodies against total Src, Fgr, Fyn, Hck, Yes, AKT were also obtained from Cell Signaling Technologies (Beverly, MA) ...
Heparanase is involved in angiogenesis in esophageal cancer through induction of cyclooxygenase-2.
Supplier
Orgel Joseph P. R. O., In PLoS ONE, 2004
... Anti-Rac 1 monoclonal antibody was purchased from Becton Dickinson Biosciences (San Diego, CA), ...
To stabilize neutrophil polarity, PIP3 and Cdc42 augment RhoA activity at the back as well as signals at the front
Supplier
Bourne Henry R. et al., In The Journal of Cell Biology, 2004
... Antibodies against Akt, phosphorylated at T308 (pAkt) and PAK and phosphorylated at S199/204 (pPAK), were obtained from Cell Signaling Technology ...
Cytochrome P450 2J2 promotes the neoplastic phenotype of carcinoma cells and is up-regulated in human tumors
Supplier
Cai L. et al., In DARU Journal of Pharmaceutical Sciences, 2004
... against Epidermal Growth Factor Receptor (EGFR), phosphorous EGFR (p-EGFR), phosphatidylinositol 3-kinase (PI3K), AKT, sEH and p-AKT were purchased from Cell Signaling Technology (Beverly, MA, USA) ...
Identification and characterization of vascular calcification-associated factor, a novel gene upregulated during vascular calcification in vitro and in vivo.
Supplier
Launikonis Bradley, In PLoS ONE, 2004
... PEBP2aA) antibody, anti-extracellular signal-regulated kinase (ERK), p-ERK, p-p38, p38, c-jun N-terminal kinase (JNK), p-JNK, Akt, p-Akt antibody, anti-mouse, and rabbit IgG peroxidase conjugate antibodies were purchased from Santa Cruz Biotechnology Inc (Waltham, MA, USA) ...
Diallyl trisulfide-induced G(2)-M phase cell cycle arrest in human prostate cancer cells is caused by reactive oxygen species-dependent destruction and hyperphosphorylation of Cdc 25 C
Supplier
Antosiewicz Jedrzej et al., In European Journal of Nutrition, 2004
... The antibodies against AKT, phospho-(Ser473)-Akt, were from Santa Cruz Biotechnology (Heidelberg, Germany) ...
Functional expression cloning reveals a central role for the receptor for activated protein kinase C 1 (RACK1) in T cell apoptosis.
Supplier
Langsley Gordon, In PLoS ONE, 2004
... subunit (clone JBS5), Akt and caspases were from Chemicon International, Santa Cruz Biotechnology (Santa Cruz, CA, USA) ...
Enhancement of sensitivity to tumor necrosis factor alpha in non-small cell lung cancer cells with acquired resistance to gefitinib
Supplier
Ueno Naoto T. et al., In Journal of Cancer, 2004
... antibodies: mouse anti-EGFR (Ab-12; Lab Vision, Fremont, CA); mouse anti-p-Akt (Ser 473, #4051) and rabbit anti-Akt (#9272; both from Cell Signaling Technology, Beverly, MA); and ...
Carbon monoxide inhalation ameliorates cold ischemia/reperfusion injury after rat liver transplantation.
Supplier
Aziz Ramy K., In PLoS ONE, 2004
... Antibodies against phospho-IκB-α (5A5), p38 MAPK, ERK1/2, JNK, Akt and IL-1β were purchased from Cell Signaling Technology (San Jose, CA) ...
Cellular targets of gefitinib.
Supplier
Zhang Lin, In PLoS ONE, 2004
... Signaling Technology, #2971), total mTOR (Cell Signaling Technology, #2983), phospho-P70S6K (T389) (Cell Signaling Technology, #9234), phospho-AKT (S473) (Cell Signaling Technology, #4051), total AKT ...
Interferon-alpha promotes the anti-proliferative effect of gefitinib (ZD 1839) on human colon cancer cell lines.
Supplier
Bauer Joseph Alan, In PLoS ONE, 2004
... Rabbit anti-Cav1/2 antibody was purchased from Transduction Laboratories (Greenland, NH) and rabbit anti-pAkt and Akt from Cell Signaling (Danvers, MA) ...
Caveolin-1 expression is critical for vascular endothelial growth factor-induced ischemic hindlimb collateralization and nitric oxide-mediated angiogenesis
Supplier
Vermes I. et al., In International Journal of Cell Biology, 2003
... Antibodies for immunodetection were from the following companies: caveolin-1 (Sigma, Mo, USA; C4490), Akt (Abcam (Cambridge, UK); ab28422), phospho-Akt ...
Endocytosis and sorting of ErbB2 and the site of action of cancer therapeutics trastuzumab and geldanamycin
Supplier
Wang Ming-Hai et al., In Molecular Cancer, 2003
... Mouse mAb to phosphor-tyrosine, Erk1/2, AKT, and other proteins were from Cell Signaling (Danvers, MA) ...
JNK suppresses apoptosis via phosphorylation of the proapoptotic Bcl-2 family protein BAD
Supplier
Ellis L M et al., In British Journal of Cancer, 2003
... as follows: polyclonal rabbit anti-phospho-ERK1/2 (p42/44) (Thr202/Tyr204; Cell Signaling Technology, Beverly, MA, USA), polyclonal rabbit anti-phospho-Akt (Ser473; Cell Signaling Technology), polyclonal rabbit anti-JNK ...
Role of endoplasmic reticulum calcium content in prostate cancer cell growth regulation by IGF and TNFalpha
Supplier
Cheng Davis W. et al., In International Journal of Biological Sciences, 2003
... Anti-cyclin D1 and phosphorylated Akt substrate motif specific antibodies were purchased form Cell Signaling Technology, Danvers, MA ...
Phase II study of Celecoxib and Trastuzumab in metastatic breast cancer patients who have progressed after prior trastuzumab based treatments
Supplier
Dunn Sandra E et al., In Breast Cancer Research, 2003
... Whole cell extracts were prepared in accordance with the protocol for P-Akt detection by Cell Signaling Technologies (CST Beverly, MA, ...
The serine/threonine kinase Akt promotes Fc gamma receptor-mediated phagocytosis in murine macrophages through the activation of p70S6 kinase
Supplier
Schneider David, In PLoS Pathogens, 2003
... Actin, phosphotyrosine, and Akt antibodies were from Santa Cruz Biotechnology (Santa Cruz, California, United ...
NF-κB is essential for epithelial-mesenchymal transition and metastasis in a model of breast cancer progression
Supplier
Hong Seong-Doo et al., In Journal of Experimental & Clinical Cancer Research : CR, 2003
... Phosphorylated Akt (Ser 473) was obtained from Cell Signaling Technology (Danvers, MA) ...
Chondroitin sulfate proteoglycans of bovine cornea: structural characterization and assessment for the adherence of Plasmodium falciparum-infected erythrocytes
Supplier
Gomes Ronald R et al., In BMC Biochemistry, 2003
... Rabbit polyclonal antibodies for phospho- and total- VEGFR-2, and Akt were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and ...
Activation of Gαi3 triggers cell migration via regulation of GIV
Supplier
Farquhar Marilyn G. et al., In The Journal of Cell Biology, 2003
... Rabbit IgG against phospho-Akt (serine 473) was purchased from Cell Signaling Technology, and mAb against ...
Persistent activation of the Akt pathwayin head and neck squamous cell carcinoma: a potential target for UCN-01
Supplier
Pinto-Júnior Décio-dos Santos et al., In Medicina Oral, Patología Oral y Cirugía Bucal, 2003
... (H-81 – Sc:15396; Santa Cruz Biotechnology, CA, USA), dilution 1:100, incubated at 4°C overnight, and anti-p-Akt 1 ⁄ 2 ⁄ 3 (Thr 308 – Sc:16646-R; Santa Cruz Biotechnology, Santa Cruz, CA, USA), ...
Bcl-x L blocks mitochondrial multiple conductance channel activation and inhibits 6-OHDA-induced death in SH-SY5Y cells.
Supplier
Tansey Malú G., In PLoS ONE, 2003
... LY294002 and the antibodies against NFκB p65, TRAF2, Akt and phospho Akt (Ser473) were from Cell Signaling Technology (Boston, MA) ...
Cathelicidins, multifunctional peptides of the innate immunity
Supplier
Ståhle M et al., In Oncogene, 2003
... Polyclonal antibodies against pERK1/2, ERK1/2, pAKT (serine 473) and AKT were purchased from Cell Signaling Technology (Danvers, MA, USA), ...
In situ measurement of degranulation as a biosensor based on RBL-2H3 mast cells.
Supplier
Zhang Xin, In PLoS ONE, 2003
... p38, JNK, Akt, Src, PLCγ1, phospho-Src (Tyr416 and 527), phospho-Paxillin (Tyr118), phospho-Erk1/2, phospho-JNK, phospho-p38 and phospho-Akt (Ser473) were from Cell Signaling Technology (Danvers, MA, USA) ...
PI3K/Akt is required for heat shock proteins to protect hypoxia-inducible factor 1alpha from pVHL-independent degradation.
Supplier
Lu Yi et al., In Molecular Vision, 2003
... LY294002, anti-Akt and anti-phospho-Akt (Ser473) antibodies were purchased from Cell Signaling Technology Inc ...
Therapy of glioblastoma multiforme improved by the antimutagenic chloroquine
Supplier
Harrison Jeffrey K., In PLoS ONE, 2002
... Akt inhibitor III (SH-6) and LY294002 were purchased from Calbiochem; PI-103 was from BioVision and NU7441 from Tocris ...
Role of SGK in hormonal regulation of epithelial sodium channel in A6 cells
Supplier
Wang Dao-Xin et al., In Respiratory Research, 2002
... were purchased from Santa Cruz Biotechnology(Santa Cruz, CA, USA) Rabbit anti- Phospho-Akt (Ser473) and total Akt monoclonal antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA) ...
Activity of TSC2 is inhibited by AKT-mediated phosphorylation and membrane partitioning
Supplier
Walker Cheryl Lyn et al., In The Journal of Cell Biology, 2002
... Biotechnology, Inc.); Rheb, phosphotuberin (T1462), S6K, phospho-S6K (T389), AKT, phospho-AKT (S473), phospho-AKT (T308), and phospho-(S/T) AKT substrate (Cell Signaling Technology); Flag M2 and ...
Irradiation differentially affects substratum-dependent survival, adhesion, and invasion of glioblastoma cell lines.
Supplier
Arendt Christopher, In PLoS ONE, 2002
... Germany), anti-caspase-3 cleaved, anti-caspase-3, anti-caspase-9 cleaved, anti-caspase-9, anti-caspase-8 cleaved, anti-caspase-8, anti-PARP cleaved, anti-PARP, anti-FADD, anti-Akt-S473, anti-Akt (Cell Signaling, Frankfurt a.M., Germany), anti-β-actin ...
Targeting I kappa B kinase for the treatment of inflammatory and other disorders
Supplier
Baharav Ehud et al., In Arthritis Research & Therapy, 2002
... total and phosphospecific PKB/Akt (S473) were purchased from Cell Signaling Technology, Inc ...
SASH1: a candidate tumor suppressor gene on chromosome 6q24.3 is downregulated in breast cancer.
Supplier
Maedler Kathrin, In PLoS ONE, 2002
... Affinity purified antibodies against total AKT and phosphorylated AKTs (Thr 308 and Ser 473) were obtained from Cell Signaling Technology Inc ...
A random variance model for detection of differential gene expression in small microarray experiments
Supplier
van de Water Bob et al., In Breast Cancer Research : BCR, 2002
... Antibodies to rabbit anti-phospho-IGF-1Rβ (Tyr1131), anti-phospho-IGF-1Rβ (Tyr1135/Tyr1136), anti-ERK1/2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-Akt and anti-phospho-Akt (Ser473) (Cell Signaling Technology Danvers, MA, USA), ...
The TSC1-2 tumor suppressor controls insulin–PI3K signaling via regulation of IRS proteins
Supplier
Lamb Richard F. et al., In The Journal of Cell Biology, 2002
... Anti-PKB, IGF-1R, pS6 (Ser240/244), PKB Ser473, and total GSK3α/β and GSK3α/β Ser21/9 were from Cell Signaling.
Role of receptor tyrosine kinases in lung cancer
Supplier
Salgia Ravi et al., In Journal of Carcinogenesis, 2002
... Phosphospecific antibodies for pS473 on AKT were obtained from Cell Signaling Technology (Beverly, MA) ...
c-Cbl is a critical modulator of the Ron tyrosine kinase receptor
Supplier
Wang Ming-Hai et al., In Molecular Cancer, 2002
... Mouse mAb specific to phospho-tyrosine (PY-100), phospho-Erk1/2, AKT, and other signaling proteins were from Cell Signaling (Danvers, MA) ...
Adherence to a Mediterranean diet and survival in a Greek population
Supplier
Li Chi-Yuan et al., In Evidence-based Complementary and Alternative Medicine : eCAM, 2002
... ), and Akt were obtained from Cell Signaling (Boston, MA, USA) ...
Tumor necrosis factor-related apoptosis-inducing ligand-induced death-inducing signaling complex and its modulation by c-FLIP and PED/PEA-15 in glioma cells
Supplier
de Bartolomeis Andrea et al., In Journal of Cellular Physiology, 2001
... Phospho-Ser473 Akt antibodies were purchased from Cell Signaling Technology (Beverly, MA) ...
Prognostic value of epidermal growth factor receptor (EGFR) and its relationship to the estrogen receptor status in 1029 patients with breast cancer
Supplier
Watson Peter H et al., In Breast Cancer Research : BCR, 2001
... A/C (1:1,000), pEGFR (phosphorylated EGFR) (1:1,000), extracellular signal-regulated kinase (ERK) (1:1,000), phosphorylated ERK (pERK) (1:1,000), AKT (1:2,000), and pAKT (1:1,000) (Cell Signaling Technology, Inc., Danvers, MA, ...
Preferential Signaling and Induction of Allergy-promoting Lymphokines Upon Weak Stimulation of the High Affinity IgE Receptor on Mast Cells
Supplier
Rivera Juan et al., In The Journal of Experimental Medicine, 2001
... Rabbit anti-phospho-Akt (Ser472/473/474) was from BD Biosciences.
Efficient expression system of human recombinant laminin-5.
Supplier
Chakravarti Shukti, In PLoS ONE, 2001
... to EGFR (Upstate, NY); a mouse mAb to phospho-ERK1/2 (E10); and, rabbit pAbs to Akt and phospho-Akt (Ser 473) from Cell Signaling Technology ...
In vitro chemo- and radio-resistance in small cell lung cancer correlates with cell adhesion and constitutive activation of AKT and MAP kinase pathways
Supplier
Song Gang et al., In OncoTargets and therapy, 2001
... ), anti-Akt, anti-rabbit IgG, and anti-mouse IgG antibodies were provided by Santa Cruz Biotechnology, Inc (Santa Cruz, CA) ...
ABC transporters as phenotypic markers and functional regulators of stem cells.
Supplier
El-Shemy Hany A., In PLoS ONE, 2001
... AKT antibody was a product of Cell Signaling Technology Inc ...
Insulin-like growth factor binding protein-1 (IGFBP-1) inhibits breast cancer cell motility
Supplier
Yee Douglas et al., In Oncogene, 2001
... Antibodies against p44/p42 ERK 1/2 (phosphospecific and total) and Akt (phosphospecific and total) were purchased from Cell Signaling (Beverly, MA) ...
PI-3K and Akt are mediators of AP-1 induction by 5-MCDE in mouse epidermal Cl41 cells
Supplier
Huang Chuanshu et al., In The Journal of Cell Biology, 2001
... antibody phosphospecific Akt (Thr308) antibody, phosphospecific Akt (Ser473) antibody, and Akt antibody were obtained from New England Biolabs, Inc ...
Rapid trafficking of membrane type 1-matrix metalloproteinase to the cell surface regulates progelatinase a activation
Supplier
Annabi Borhane et al., In Journal of Cell Communication and Signaling, 2001
... The polyclonal antibodies against AKT and phosphorylated-AKT were purchased from Cell Signaling (Danvers, MA) ...
The role of the cyclin-dependent kinase inhibitor p21 in apoptosis.
Supplier
Bunting Kevin D., In PLoS ONE, 2001
... Cleaved-caspase-3, Cleaved-caspase-7, Cleaved-capase-9, and phospho-Akt (Ser473) were from Cell Signaling (Beverly, MA) ...
The phosphatidylinositol 3-kinase-AKT pathway in human cancer
Supplier
Prata Cecilia et al., In Oxidative Medicine and Cellular Longevity, 2001
... Anti-caspase 3, anti-Bax, anti-Bcl-2, and anti-p-Akt antibodies were purchased from Cell Signaling Technology ...
A role for the myoglobin redox cycle in the induction of endothelial cell apoptosis
Supplier
Kwon Hee Uk et al., In Korean Journal of Anesthesiology, 2001
... Anti-p-Akt antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA) ...
Divergent optimum levels of lycopene, beta-carotene and lutein protecting against UVB irradiation in human fibroblastst
Supplier
Wu Wen-Bin et al., In Journal of Biomedical Science, 2001
... ), total p38 MAPK, phospho-p38 MAPK and total Akt were from Cell Signaling Technology, Inc ...
Type I phosphatidylinositol 4-phosphate 5-kinase controls neutrophil polarity and directional movement
Supplier
Mañes Santos et al., In The Journal of Cell Biology, 2001
... -MLC, -phospho AKT, and -spectrin αII were from Cell Signaling Technology; anti-Rac and anti-AKT ...
c-myc is a downstream target of the Smad pathway
Supplier
Datta P K et al., In British Journal of Cancer, 2001
... purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and anti-phospho-Smad2, anti-phospho-ERK, anti-phospho-Rb, anti-phospho-AKT and AKT antibodies were purchased from Cell Signaling (Danvers, MA, USA) ...
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
Supplier
Pesce Maurizio, In PLoS ONE, 2000
... Lakes, NJ, USA); the monoclonal antibodies to phosphorylated and non-phosphorylated forms of ERK1/2, p38, JNK, Akt and anti-rabbit IgG-HPR from Cell Signaling Technology (Beverly, MA, USA); ...
Covalently conjugated VEGF—fibrin matrices for endothelialization
Supplier
Iruela-Arispe M. Luisa et al., In The Journal of Cell Biology, 2000
... Rabbit anti–human VEGFR 2, phospho-VEGFR 2 (Tyr996 and Tyr1175), p38 MAPK, phospho-p38 MAPK (Thr180/Tyr182), Akt, and phospho-Akt (S473) were from Cell Signaling Technology ...
High-performance liquid chromatography-based determination of total isothiocyanate levels in human plasma: application to studies with 2-phenethyl isothiocyanate
Supplier
Xu Ke et al., In BMC Cancer, 2000
... Rabbit monoclonal antibodies against Twist, MMP-2, polyclonal antibodies against Akt, p-Akt were purchased from Cell Signaling (Beverly, MA), mouse monoclonal ...
Ten years of protein kinase B signalling: a hard Akt to follow
Supplier
Krügel U. et al., In Purinergic Signalling, 2000
... origin, recommended for the detection of Ser 473 phosphorylated Akt 1 and corresponding phosphorylated Akt 2 and Akt 3 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, ...
Electrostatics of nanosystems: Application to microtubules and the ribosome
Supplier
Kuriyan John, In PLoS Biology, 2000
... Phospho-Akt Thr308 and Ser473 as well as pan Akt were from Cell Signaling (New England BioLabs UK, ...
VEGF receptor signal transduction.
Supplier
Kume Tsutomu, In PLoS ONE, 2000
... Tyr 1175, Cell Signaling #2478S; Tyr 1214, Upstate 07-374); rabbit anti-VEGFR2 (Cell Signaling #2479); rabbit anti-phospho-Akt (Ser 473, Cell Signaling #9271S); rabbit anti-Akt (Cell ...
The B Cell Antigen Receptor Controls Integrin Activity through Btk and PLCγ2
Supplier
Pals Steven T. et al., In The Journal of Experimental Medicine, 1999
... the phosphorylation state-specific antibodies phospho-p44/42 MAP kinase [T202/Y204] against ERK1 and 2 and phospho-Akt [Ser473] against PKB/Akt (New England Biolabs); anti-ERK2 (C-14) and anti-Akt1/2 ...
Tau protein is hyperphosphorylated in a site-specific manner in apoptotic neuronal PC12 cells
Supplier
Jin Byung K et al., In BMC Neuroscience, 1999
... Louis, MO, USA), rabbit anti-phospho-Akt (Ser473), rabbit anti-phospho-Akt (Thr308), rabbit anti-phospho-GSK-3β (Ser9), rabbit anti-Akt and rabbit anti-GSK-3β (Cell Signaling Technology, MA, USA), mouse ...
Role of the pleckstrin homology domain of PLCγ1 in its interaction with the insulin receptor
Supplier
Bernier Michel et al., In The Journal of Cell Biology, 1999
... The phospho-p42/44 MAPK and phospho-AKT antibodies were purchased from Cell Signaling Technology ...
Munc-18 associates with syntaxin and serves as a negative regulator of exocytosis in the pancreatic beta-cell
Supplier
Thurmond Debbie C. et al., In The Journal of Cell Biology, 1999
... Rabbit polyclonal antiphospho-Akt (Thr-308) and Akt (pan) antibodies were purchased from Cell Signaling Technology ...
Expression of Fas ligand in arteries of hypercholesterolemic rabbits accelerates atherosclerotic lesion formation.
Supplier
Rieux-Laucat Frederic, In PLoS ONE, 1999
... For Western blotting experiments, anti-GAPDH, anti-PI3K, anti-HSC70 were obtained from Santa Cruz Biotechnology (CA, USA), anti-phospho-Akt from Upstate (Milipore, Molsheim, France), anti-Akt, and ...
The PI3 kinase, p38 SAP kinase, and NF-κb signal transduction pathways are involved in the survival and maturation of lipopolysaccharide-stimulated human monocyte-derived dendritic cells
Supplier
Kuan Yu-Hsiang et al., In Evidence-based Complementary and Alternative Medicine : eCAM, 1999
... Rabbit polyclonal antibodies to phospho-Akt were purchased from Cell Signaling Technology ...
Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence
Supplier
Sessa William C. et al., In The Journal of Cell Biology, 1999
... Antibodies against Akt, phospho-Akt, and the senescence β-galactosidase staining kit were obtained from Cell Signaling Technology ...
Lack of elevated MAP kinase (Erk) activity in pancreatic carcinomas despite oncogenic K-ras expression
Supplier
Salh B et al., In British Journal of Cancer, 1998
... Anti-phosphospecific-PKB Ser473 and GSK3β Ser-9 were obtained from New England Biolabs Inc ...
Bcl-2 inhibits early apoptotic events and reveals post-mitotic multinucleation without affecting cell cycle arrest in human epithelial tumor cells exposed to etoposide
Supplier
Ko Kwang Ho et al., In Journal of Biomedical Science, 1998
... The antibodies for Western blotting, p-PI3K, PI3K, p-Akt, and Akt were purchased from Cell Signaling (Beverly, MA) ...
Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
Supplier
Chang Moon-Jeong et al., In Nutrition Research and Practice, 1998
... (Santa Cruz, CA, USA) and antibody for Akt was purchased from Cell Signaling Technology Inc ...
An APAF-1 cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9
Supplier
Pittman Randall N. et al., In The Journal of Cell Biology, 1998
... Rabbit polyclonal antibody to Akt and phosphospecific antibody for Akt Serine-473 were from New England Biolabs, Inc., and a rabbit polyclonal ...
Analysis of gene expression in single oocytes and embryos by real-time rapid cycle fluorescence monitored RT-PCR.
Supplier
Giniger Edward, In PLoS ONE, 1998
... α-ActRIIB (H70) (Santa Cruz), mouse α-BMPRII (BD Transduction Laboratories), rabbit α-phospho-Smad1/5/8, rabbit α-phospho-Akt(S) and rabbit α-Akt (Cell Signaling Technology), rabbit α-Smad1 (Upstate ...
Absence of the tight junctional protein AF-6 disrupts epithelial cell-cell junctions and cell polarity during mouse development
Supplier
Miyoshi Jun et al., In Journal of Cell Science, 1998
... Tokyo, Japan), Ki-67 (Novocastra Laboratories, Newcastle upon Tyne, UK), JAM-A (R&D Systems), Akt, and phosphorylated Akt (Cell Signaling Technology) ...
Endostatin inhibits VEGF-induced endothelial cell migration and tumor growth independently of zinc binding.
Supplier
Lamartiniere Coral A. et al., In Environmental Health Perspectives, 1998
... (EGFR), erbB2, phosphorylatederbB3, phosphorylatederbB3, insulin-like growth factor 1 receptor (IGF-1R), phosphorylated-IGF-1R, phosphorylated-Bad, PI3K, PTEN, Akt 1, Akt 3, phosphorylatedAkt, glycogen synthase kinase-3-beta (GSK-3β), and phosphorylatedGSK-3β were purchased from Cell Signaling Technologies (Danvers, MA) ...
Regulation of Ras–MAPK pathway mitogenic activity by restricting nuclear entry of activated MAPK in endoderm differentiation of embryonic carcinoma and stem cells
Supplier
Xu Xiang-Xi et al., In The Journal of Cell Biology, 1998
... monoclonal (Sigma-Aldrich); and Erk2/1 polyclonal, phospho-p90RSK (Ser 380) polyclonal, phospho-p90RSK (Thr 359/Ser 363) polyclonal, and phospo-AKT (Ser 473) polyclonal (Cell Signaling Technology) ...
The neuronal scaffold protein Shank3 mediates signaling and biological function of the receptor tyrosine kinase Ret in epithelial cells
Supplier
Birchmeier Walter et al., In The Journal of Cell Biology, 1998
... from R&D Systems, antibodies to active Erk–MAPK and Flag tag from Sigma-Aldrich, antibodies to active Akt pS473 from Cell Signaling, and anti–Akt-PKBα and pan–Erk ...
Cytolytic T lymphocyte-associated antigen-4 and the TcRζ/CD3 complex, but not CD28, interact with clathrin adaptor complexes AP-1 and AP-2.
Supplier
Wölfl Stefan, In PLoS ONE, 1998
... and anti-phospho-BAD (Ser-136) mAbs from Biosource (Nivelles, Belgium), anti-AKT1/2 from Autogen Bioclear (Wiltshire, UK), anti-phospho AKT (Thr-308) from New England Biolabs (Hertfordshire, UK), anti-BcL-XL from ...
Krox-20 inhibits Jun-NH2-terminal kinase/c-Jun to control Schwann cell proliferation and death
Supplier
Jessen Kristjan R. et al., In The Journal of Cell Biology, 1998
... Antibodies against ERK1/2, phosphorylated JNK1/2 (Thr183/Tyr185), JNK1/2, and Ser473 phospho-Akt were purchased from Cell Signaling Technology (New England Biolabs, ...
Akt2 phosphorylates Synip to regulate docking and fusion of GLUT4-containing vesicles
Supplier
Mori Masatomo et al., In The Journal of Cell Biology, 1997
... Phosphospecific Akt substrate antibody was purchased from Cell Signaling Technology, and Akt1- and ...
The p85 regulatory subunit of phosphoinositide 3-kinase down-regulates IRS-1 signaling via the formation of a sequestration complex
Supplier
Cantley Lewis C. et al., In The Journal of Cell Biology, 1997
... Upstate Biotechnology; anti-EEA1 and anti–caveolin-1 antibodies were from Transduction Laboratories; anti–phospho-Akt serine-473, anti–phospho-Akt threonine-308, and anti-Akt antibodies were from Cell Signaling Technology; anti-HA antibody was ...
Class II phosphoinositide 3-kinase defines a novel signaling pathway in cell migration
Supplier
Falasca Marco et al., In The Journal of Cell Biology, 1997
... L-α-LPA, oleoyl (C18:1,[cis]-9), anti-myc, anti–β-actin, and anti-Flag were purchased from Sigma-Aldrich; anti-Akt, anti-pSer473Akt, and anti-pThr308 from Santa Cruz Biotechnology, Inc.; anti-HA from Boehringer; ...
Inositol 1,4,5-trisphosphate [correction of tris-phosphate] activation of inositol trisphosphate [correction of tris-phosphate] receptor Ca2+ channel by ligand tuning of Ca2+ inhibition
Supplier
Stöckli Jacqueline et al., In The Journal of Biological Chemistry, 1997
... Akt (11E7), Akt2 (D6G4), and total GSK3β (27C10), and monoclonal mouse antibodies raised against pSer-473 Akt (587F11), Akt1 (2H10), and pTyrosines (pY100) were purchased from Cell Signaling Technologies (Beverly, MA) ...
Persistent activation of RelA by respiratory syncytial virus involves protein kinase C, underphosphorylated IkappaB-beta, and sequestration of protein phosphatase 2A by the viral phosphoprotein
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Barik Sailen et al., In BMC Microbiology, 1997
... Antibodies against the following antigens were obtained from commercial sources: p65, Sp1 (Santa Cruz Biotechnology); phospho-AKT, caspases-3, -6, -7, -8, -9 (Cell Signaling Technology); caspase-10 (Oncogene); caspase-12 ...
Lipid raft microdomain compartmentalization of TC10 is required for insulin signaling and GLUT4 translocation
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Pessin Jeffrey E. et al., In The Journal of Cell Biology, 1997
... The transferrin receptor, phospho-PKB (Ser473), and HA epitope tag antibodies were obtained from Leinco Technologies, Cell Signaling Technology, and ...
Regulation of the p85/p110 phosphatidylinositol 3'-kinase: stabilization and inhibition of the p110alpha catalytic subunit by the p85 regulatory subunit
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Yu Dihua et al., In Oncogene, 1997
... were then incubated with 14-3-3ζ polyclonal antibody (C-16; Santa Cruz, Santa Cruz, CA, USA) or phospho-Akt Ser473 (Cell Signaling, Danvers, MA, USA) at ...
Protein kinase B (c-Akt)a multifunctional mediator of phosphatidylinositol 3-kinase activation
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Pals Steven T. et al., In The Journal of Experimental Medicine, 1997
... Biotechnology, Inc.); rabbit anti–Grb2-associated binder 1 (Gab1; Upstate Biotechnology); rabbit anti–stromal cell–derived factor (SDF)-1α (PeproTech); rabbit anti-Akt (H-136; Santa Cruz Biotechnology, Inc.); phospho-specific rabbit anti-Akt ...
An intracellular signaling hierarchy determines direction of migration in opposing chemotactic gradients
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Kubes Paul et al., In The Journal of Cell Biology, 1997
... Rabbit anti–phospho-Akt (Ser 473) antibodies and HRP-conjugated anti–rabbit IgG antibodies were purchased from Cell Signaling.
Obesity-related overexpression of fatty-acid synthase gene in adipose tissue involves sterol regulatory element-binding protein transcription factors
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Hajduch Eric et al., In Diabetes, 1997
... Antibodies against caveolin-1 were purchased from BD Biosciences; native PKB/Akt, Ser473-PKB, Thr308-PKB, Ser21/9–glycogen synthase kinase 3α/β (GSK3α/β), and Thr410/403-PKCζ/λ were from Cell Signaling (New England Biolabs); and ...
Nicotinic acetylcholine receptor is internalized via a Rac-dependent, dynamin-independent endocytic pathway
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Barrantes Francisco J. et al., In The Journal of Cell Biology, 1996
... Antibodies against c-Src and pTyr418-Src were obtained from Cell Signaling Technology and Millipore, anti-Rac was obtained from BD Biosciences, and anti–lysobisphosphatidic acid antibody ...
Negative Regulation of Immunoglobulin E–dependent Allergic Responses by Lyn Kinase
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Rivera Juan et al., In The Journal of Experimental Medicine, 1996
... Rabbit anti–phospho-Akt (Ser472/473/474) was from BD Biosciences.
Misexpression of chick Vg1 in the marginal zone induces primitive streak formation
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Dodd Jane et al., In Neural Development, 1996
... Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit α-phospho-Smad1/5/8 (pSmad); rabbit α-phospho-Akt(S) (pAkt) and rabbit α-Akt (Cell Signaling Technology); mouse α-flag (M2; ...
Effects of transforming growth factor-beta and platelet-derived growth factor on oligodendrocyte precursors: insights gained from a neuronal cell line.
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Mei Lin, In PLoS ONE, 1996
... Rabbit polyclonal antibodies against pERK, ERK, pAkt and Akt were from Cell Signaling.
STK/RON receptor tyrosine kinase mediates both apoptotic and growth signals via the multifunctional docking site conserved among the HGF receptor family
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Wang Ming-Hai et al., In Journal of Experimental & Clinical Cancer Research : CR, 1995
... goat or rabbit IgG antibodies specific to regular or phosphor- Erk1/2 (p44/42), p38 MAP kinase, AKT, GSK-3β, and β-catenin were from Cell Signaling Inc (Beverly, MA) ...
Peptidase inhibitors improve recovery of substance P and calcitonin gene-related peptide release from rat spinal cord slices
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Hingtgen Cynthia M et al., In Molecular Pain, 1995
... titer, Cell Signaling Technologies; Erk 1/2: rabbit polyclonal primary antibody, 1:900 titer, Cell Signaling Technologies; p-Akt: mouse monoclonal primary antibody, 1:500 titer, Cell Signaling Technologies; Akt: rabbit polyclonal ...
Serine phosphorylation of death agonist BAD in response to survival factor results in binding to 14-3-3 not BCL-X(L)
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Cantrell Doreen et al., In The Journal of Cell Biology, 1995
... The serine 473 phosphospecific PKB antibody was purchased from New England Biolabs ...
The stress activated protein kinase pathway mediates cell death following injury induced by cis-platinum, UV irradiation or heat
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Adler G et al., In British Journal of Cancer, 1995
... and anti-AKT antibodies were from New England Biolabs (Schwalbach/Taunus, Germany) ...
Atypical protein kinase C (PKCζ/λ) is a convergent downstream target of the insulin-stimulated phosphatidylinositol 3-kinase and TC10 signaling pathways
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Pessin Jeffrey E. et al., In The Journal of Cell Biology, 1995
... Antibodies against GSK-3β phosphoserine-9 and PKB phosphothreonine-308 and phosphoserine-473 antibodies were obtained from Cell Signaling Technology ...
VMD: visual molecular dynamics
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Toker Alex, In PLoS Biology, 1995
... Okadaic acid sodium salt and AKT inhibitor VIII were from Calbiochem (Merck KGaA) ...
Novel role of neuronal Ca2+ sensor-1 as a survival factor up-regulated in injured neurons
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Nabekura Junichi et al., In The Journal of Cell Biology, 1994
... Akt/PKBα cDNA was cloned from the human kidney cDNA library (CLONTECH Laboratories, Inc.), and NH ...
Integrin-dependent activation of MAP kinasea link to shape-dependent cell proliferation
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Le T. Duong et al., In The Journal of Cell Biology, 1994
... Anti-Akt/PKB and anti–phospho-Akt/PKB antibodies were from New England Biolabs, Inc ...
Integrin transmembrane signaling and cytoskeletal control
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Yamada Kenneth M. et al., In The Journal of Cell Biology, 1994
... kinase antibody and p44/42 MAP kinase assay kits, as well as polyclonal anti-phospho-MEK1, anti-phospho-Akt, and anti-Akt were purchased from New England BioLabs, Inc ...
Requirement of dendritic Akt degradation by the ubiquitin–proteasome system for neuronal polarity
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Wang Yizheng et al., In The Journal of Cell Biology, 1993
... and mouse monoclonal anti-GFP, -Tuj1, -MAP2, or -Tau1 antibodies (Chemicon); rabbit anti–p-Akt (Ser473), p–GSK-3β (Ser9), anti-Akt, or –GSK-3β antibodies (Cell Signaling Technology); mouse anti-myc or ...
Thyroid tumorigenesis
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Franc B et al., In British Journal of Cancer, 1993
... Fremont, CA, USA); anti-Phospho-p44/42 MAPK Thr202/Tyr204 monoclonal rabbit antibody (20G11, Cell Signalling, Danvers, MA, USA), anti-Phospho-Akt Ser473 monoclonal rabbit monoclonal antibody (736E11, Cell Signaling).
Rapid activation of the STAT3 transcription factor by granulocyte colony-stimulating factor
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Nerurkar Pratibha V., In PLoS ONE, 1993
... Mouse monoclonal antibody against GAPDH and rabbit polyclonal anti-human Akt were purchased from Santa Cruz Biotechnology Inc ...
Safety of esmolol in patients with acute myocardial infarction treated with thrombolytic therapy who had relative contraindications to beta-blocker therapy
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Fujise Kenichi et al., In Cardiovascular Drugs and Therapy, 1993
... Dilution for anti-pAkt and anti-Akt (Santa Cruz Biotechnology, Santa Cruz, CA) was ...
Active site labeling of the Yersinia protein tyrosine phosphatase: the determination of the pKa of the active site cysteine and the function of the conserved histidine 402
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Neumann Carola A et al., In The EMBO Journal, 1992
... Ly294002, WM, antibodies against PTEN (138G6), Akt (11-E7), Akt-phosphoserine 473 (193H12), Akt-phosphothreonine 308 (244F9), phospho Akt substrate (110B7E) and HA-tag were purchased from Cell Signaling; Antibodies recognizing PTEN (A2B1), ...
Inactivation of glycogen synthase kinase-3 beta by phosphorylation: new kinase connections in insulin and growth-factor signalling.
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Koch Karl-Wilhelm, In PLoS ONE, 1992
... Antibodies to phospho-AKT Ser473, phospho-GSK3β Ser9 and AKT were obtained from Cell signaling (Boston, MA) ...
The identification and characterization of an actin-binding site in α-actinin by mutagenesis.
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Cordes Nils, In PLoS ONE, 1991
... Immunoblots for ICAM-2, α-actinin, ezrin, actin, AKT, and phospho-AKT were done with AF244 (R&D Systems, Minneapolis, MN) and C-20 (Santa Cruz Biotechnologies, Beverly, MA), CBL231 (Research Diagnostics, Inc., Flanders, NJ), C-20 ...
An inactive pool of GSK-3 at the leading edge of growth cones is implicated in Semaphorin 3A signaling
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Doherty Patrick et al., In The Journal of Cell Biology, 1989
... sheep antibodies recognizing GSK-3α and P-(Ser21)-GSK-3α (lot 18087) were purchased from Upstate Biotechnology, and the P-AKT antibody was purchased from Cell Signaling Technology ...
Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
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Hotchin Neil A., In PLoS ONE, 1987
... (pY397) (BD Biosciences), rabbit anti-human p-GSK3β (Ser9), anti-human total GSK3β, anti-human p-Akt (Ser473), anti-human total Akt (Cell Signaling), and anti-human actin serum ...
Temperature-dependent physiological stability of rat skeletal muscle in vitro.
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Vina Jose, In PLoS ONE, 1984
... (#9572), secondary antibodies conjugated with horseradish peroxidase (HRP) (anti-rabbit (#7074) or anti-mouse (#7076)) and nonradioactive Akt kinase assay kit (#9840) were purchased from Cell Signaling Technology (Beverly, MA) ...
Measurement of protein using bicinchoninic acid.
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Plow Edward F., In PLoS ONE, 1984
... Polyclonal ADAM17 antibody raised in rabbit was purchased from Calbiochem (Nottingham, UK), while Akt antibody raised in rabbit was purchased from Cell Signaling (Hertfordshire, UK) ...
Bombesin-like peptides can function as autocrine growth factors in human small-cell lung cancer
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Hellmich Mark R et al., In BMC Molecular Biology, 1984
... Antibodies to Akt and phosphor-Akt were purchased from Cell Signaling Technology, Inc ...
Developments of a water-maze procedure for studying spatial learning in the rat.
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Priller Josef, In PLoS ONE, 1983
... human fibroblast growth factor 2 (FGF-2) was from Millipore (Temecula, CA); Antibodies for ERK1/2, phospho-ERK1/2, Akt, phosphor-Akt (Ser-473) were obtained from Cell Signaling Technology (Beverly, MA) ...
Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation
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Colombo Giorgio, In PLoS ONE, 1982
... rabbit polyclonal IgG antibodies against phospho-p44/42 mitogen-activated protein kinase (MAPK), p44/42 MAPK, phospho-Akt HM(S473), and Akt HM(S473) were purchased from Cell Signaling Technology (Beverly, MA, US) ...
Insulin resistance in adipocytes from fed and fasted obese rats: Dissociation of two insulin actions.
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Wong Nai Sum, In PLoS ONE, 1980
... Antibodies to phospho-Akt (Thr473) phospho-Akt (Ser473), and total Akt, were obtained from Cell Signaling Technologies (Danvers, MA) ...
Evidence that vascular endothelial cells can induce the retraction of fibrin clots.
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Blagosklonny Mikhail, In PLoS ONE, 1980
... Anti Akt and anti-phospho Akt (pS473) polyclonal antibodies were from Cell Signaling Technology (Beverly, MA) ...
Inhibition of endothelial cell migration by thrombospondin-1 type-1 repeats is mediated by β1 integrins
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Zetter Bruce R. et al., In The Journal of Cell Biology, 1979
... Phosphorylated Akt (ser473) antibody was from Cell Signaling and antibody against total ...
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
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Gold Jeffrey A., In PLoS ONE, 1975
... beads from BioRad; Wortmannin, LY294002 and zymosan from ICN Biomedical Inc.; anti-phospho Akt and total Akt from New England Biolabs; genistein, protease inhibitor cocktail ...
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