Surface expression of ω-transaminase in Escherichia coli.
Stockholm, Sweden. In Appl Environ Microbiol, 03 Mar 2014
However, transaminases need to dimerise in order to become active, and furthermore require the cofactor pyridoxal phosphate, and consequently successful transaminase surface expression has not been reported to date.In this work, we produced an Arthrobacter citreus ω-transaminase in Escherichia coli using a surface display vector based on the autotransporter Adhesin Involved in Diffuse Adherence (AIDA-I), which has previously been used for display of dimeric proteins.
Frequency of virulence factors in Escherichia coli isolated from suckling pigs with diarrhoea in China.
Harbin, China. In Vet J, 28 Feb 2014
A total of 381 E. coli strains, obtained from 290 faecal samples from pigs on 38 farms, were tested for fimbriae (K88, K99, 987P, F41, F18, F17), non-fimbrial adhesins (AIDA-I, paa, CS31A, eae, saa), enterotoxin (LT-I, LT-II, STa, STb, EAST1), Shiga toxin (Stx1, Stx2, Stx2e), pathogenicity islands (HPI, LEE), α-haemolysin (hlyA), afa8 gene cluster (afaD, afaE) and sepA genes by PCR.
Interaction of DHPG-LTD and synaptic-LTD at senescent CA3-CA1 hippocampal synapses.
Gainesville, United States. In Hippocampus, 04 Feb 2014
Application of individual antagonist for the group I mGluR (AIDA), the N-methyl-d-aspartate receptor (NMDAR) (AP-5), or L-type voltage-dependent Ca(2+) channel (VDCC) (nifedipine) failed to block synaptic-LTD and any two antagonists severely impaired synaptic-LTD induction, indicating that activation of any two mechanisms is sufficient to induce synaptic-LTD in aged animals.
STb and AIDA-I: the missing link?
Saint-Hyacinthe, Canada. In Crit Rev Microbiol, 2010
With the recent recognition of a new adhesin, originally found in human E. coli isolates, named AIDA (adhesin involved in diffuse adherence) and its association with new E. coli pathotypes to which STb is linked, new light was shed on STb toxic potency.