Binding rate constants reveal distinct features of disordered protein domains.
In Biochemistry, 08 Aug 2015
We have performed an extensive characterization of the ionic strength dependence of the interaction between the molten globular nuclear co-activator binding domain (NCBD) of CREB binding protein and five different protein ligands, including the intrinsically disordered activation domain of p160 transcriptional co-activators (SRC1, TIF2, ACTR), the p53 transactivation domain and the folded pointed domain (PNT) of the transcription factor ETS-2. Direct comparisons of the binding rate constants under identical conditions show that the association rate constant, kon, for interactions between NCBD and disordered protein domains is high at low salt concentrations (90-350×106 M-1s-1 at 4°C) but is reduced significantly (10- to 30-fold) with increasing ionic strength and reaching a plateau around physiological ionic strength.
Genetically engineered ERα-positive breast cancer mouse models.
Washington, D.C., United States. In Endocr Relat Cancer, Jun 2014
ESR1, CCDN1, prolactin, TGFα, AIB1, ESPL1, and WNT1 overexpression, PIK3CA gain of function, as well as loss of P53 (Trp53) or STAT1 are associated with ER+ mammary cancer.
New targets for the antitumor activity of gambogic acid in hematologic malignancies.
Wuhan, China. In Acta Pharmacol Sin, 2013
These include the regulation of expression and intracellular positioning of nucleoporin and nucleophosmin; downregulation of steroid receptor coactivator-3 (SRC-3) and its downstream proteins; upregulation of death inducer-obliterator (DIO-1); downregulation of HERG potassium channel; as well as induction of reactive oxygen species (ROS) accumulation.