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ADE2 Ade2p

ade2
This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] (from NCBI)
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Top mentioned proteins: CAN, ACID, HAD, LEU2, SIMPLE
Papers on ade2
Screening and verification of proteins that interact with HSPC238.
New
Yuan et al., Shiqi, China. In Oncol Rep, Dec 2015
Multiple reporter gene assays with LacZ, HIS and ADE2 selective media identified 12 genes.
Homology-integrated CRISPR-Cas (HI-CRISPR) system for one-step multigene disruption in Saccharomyces cerevisiae.
New
Zhao et al., Urbana, United States. In Acs Synth Biol, Jun 2015
As proof of concept, three genes, CAN1, ADE2, and LYP1, were simultaneously disrupted in 4 days with an efficiency ranging from 27 to 87%.
Adenine auxotrophy--be aware: some effects of adenine auxotrophy in Saccharomyces cerevisiae strain W303-1A.
Liepins et al., Rīga, Latvia. In Fems Yeast Res, 2014
Strain W303-1A, which carries the ade2-1 mutation, is widely used in physiological and genetic research.
Is the alcohol deprivation effect genetically mediated? Studies with HXB/BXH recombinant inbred rat strains.
Tabakoff et al., Aurora, United States. In Alcohol Clin Exp Res, 2014
Broad-sense heritability of the ADEs was low (ADE1, 9.1%; ADE2, 26.2%; ADE3, 16.3%).
PRMT3: new binding molecule to RhoGDI-α during mycophenolic acid-induced β-cell death.
Kim et al., Seoul, South Korea. In Transplant Proc, 2014
Y2H screening of RhoGDI-α was performed in yeast PBN204 strain containing 3 reporters (URA3, lacZ, and ADE2) under the control of different GAL promoters.
Fitness costs of minimal sequence alterations causing protein instability and toxicity.
Korona et al., Kraków, Poland. In Mol Biol Evol, 2014
We derived collections of temperature-sensitive, and thus structurally unstable, mutants of the yeast ADE2 and LYS2 genes by introducing single or very few amino acids substitutions.
Evaluation of the lower protein limit in the budding yeast Saccharomyces cerevisiae using TIPI-gTOW.
Moriya et al., Okayama, Japan. In Bmc Syst Biol, 2013
We first tested the feasibility of this method using ADE2 as a marker and then analyzed some cell cycle regulators to reveal genetic interactions.
A novel platform for heterologous gene expression in Trichoderma reesei (Teleomorph Hypocrea jecorina).
Mortensen et al., Denmark. In Microb Cell Fact, 2013
The ade2 locus was selected as integration site since ade2 mutants develop red pigment that facilitates easy and rapid detection of correctly targeted transformants.
A link between impaired purine nucleotide synthesis and apoptosis in Drosophila melanogaster.
GeneRIF
Clark et al., Fredericton, Canada. In Genetics, 2011
Data show the presence of apoptosis at the cellular level in both ade2 and Prat mutants, and the upregulated gene HtrA2, which encodes an apoptosis effector and is thus a candidate for initiating apoptosis in response to purine depletion.
Determination of the differential estrogenicity of isoflavonoids by E2-ER-ERE-dependent gene expression in recombinant yeast and MCF-7 human breast cancer cells.
Teng et al., Taipei, Taiwan. In Food Chem, 2008
In this study, differential estrogenicities of five isoflavonoids found in red clover and soy, including biochanin A, daidzein, formononetin, genistein and glycitein were examined in a yeast-based screen system with a classical palindromic estrogen response element (ERE)-ADE2 reporter and in a MCF-7 cell culture system with mRNA levels of ER-dependent genes compared.
Production of knockout rats using ENU mutagenesis and a yeast-based screening assay.
Impact
Gould et al., Madison, United States. In Nat Biotechnol, 2003
F1 preweanling pups from mutagenized Sprague Dawley (SD) male rats were then screened for functional mutations in Brca1 and Brca2 using a yeast gap-repair, ADE2-reporter truncation assay.
Break-induced replication: a review and an example in budding yeast.
Review
Haber et al., Waltham, United States. In Proc Natl Acad Sci U S A, 2001
In other experiments, cells were transformed with DNA fragments lacking an ARS and homologous to only 50 bp of ADE2 added to the ends of a URA3 gene.
PCNA connects DNA replication to epigenetic inheritance in yeast.
Impact
Stillman et al., New York City, United States. In Nature, 2000
The pol30-8 mutant displayed coexistence of both repressed (pink) and de-repressed (white) cells within a single colony when assayed with the ADE2 gene inserted at HMR.
Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription.
Impact
Zakian et al., Chicago, United States. In Cell, 1990
For example, cells bearing a telomere-linked copy of ADE2 produced predominantly red colonies (a phenotype characteristic of ade2- cells) containing white sectors (characteristic of ADE2+ cells).
Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss.
Impact
Davis et al., In Cell, 1985
In diploid strains homozygous for an ochre mutation in ade2, cells carrying no copies of the SUP11 gene are red, those carrying one copy are pink, and those carrying two or more copies are white.
Genetic analysis of yeast RAS1 and RAS2 genes.
Impact
Wigler et al., In Cell, 1984
We have determined that RAS1 is located on chromosome XV, 7 cM from ade2 and 63 cM from his3; and RAS2 is located on chromosome XIV, 2 cM from met4 .
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