Serine/threonine ligation for the chemical synthesis of proteins.
Hong Kong, Hong Kong. In Curr Opin Chem Biol, 2014
Ser/Thr ligation has been used for the synthesis of the human erythrocyte acylphosphatase protein and MUC1 glycopeptide segments, semisynthesis of peptoid/PEG-RNase S protein hybrids, and cyclic peptide synthesis including cyclic tetrapeptides, cyclomontanin B, yunnanin C, mahafacyclin B, and daptomycin.
Protein chemical synthesis by serine and threonine ligation.
Hong Kong, Hong Kong. In Proc Natl Acad Sci U S A, 2013
The utility of this peptide ligation approach has been demonstrated through the convergent syntheses of two therapeutic peptides--ovine-corticoliberin and Forteo--and the human erythrocyte acylphosphatase protein (∼11 kDa).
Early hemodynamic and biochemical changes in overloaded swine ventricle.
Florence, Italy. In Tex Heart Inst J, 2012
The present study was undertaken to investigate, in an animal model, the relationship between sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) activity, phospholamban phosphorylation, acylphosphatase activity, and hemodynamic changes that occur in the early phase of pressure overload.
Enzyme activity in the crowded milieu.
Troy, United States. In Plos One, 2011
Here we chose to use the synthetic crowding agent Ficoll, which is commonly used to mimic cytosolic crowding conditions to study the crowding effect on the catalytic properties of glycolytic enzymes, namely phosphoglycerate kinase, glyceraldehyde 3-phosphate dehydrogenase, and acylphosphatase.