Optimal isotope labelling for NMR protein structure determinations.
Hachiōji, Japan. In Nature, 2006
We demonstrate for the 17-kDa protein calmodulin and the 41-kDa maltodextrin-binding protein that SAIL offers sharpened lines, spectral simplification without loss of information, and the ability to rapidly collect the structural restraints required to solve a high-quality solution structure for proteins twice as large as commonly solved by NMR.
Genetic engineering of proteins with cell membrane permeability.
Nashville, United States. In Nat Biotechnol, 1998
To explore the applicability of this nondestructive import method to the study of intracellular processes, a 41-kDa GST-Grb2SH2-MTS fusion protein containing the Grb2 SH2 domain was tested for its effect on the epidermal growth factor (EGF)-stimulated signaling pathway.