Biosynthesis of enantiopure (S)-3-hydroxybutyric acid in metabolically engineered Escherichia coli.
Taejŏn, South Korea. In Appl Microbiol Biotechnol, 2008
A biosynthetic pathway for the production of (S)-3-hydroxybutyric acid (S3HB) from glucose was established in recombinant Escherichia coli by introducing the beta-ketothiolase gene from Ralstonia eutropha H16, the (S)-3-hydroxybutyryl-CoA dehydrogenase gene from R. eutropha H16, or Clostridium acetobutylicum ATCC824, and the 3-hydroxyisobutyryl-CoA hydrolase gene from Bacillus cereus ATCC14579.
Catabolism of isobutyrate by colonocytes.
Indianapolis, United States. In Arch Biochem Biophys, 1996
Both enterocytes and colonocytes express high levels of 3-hydroxyisobutyryl-CoA hydrolase, an enzyme activity important in maintaining low intracellular concentrations of methacrylyl-CoA, a common, potentially toxic intermediate in the catabolic pathways of these compounds.